Báo cáo khoa học: Ribonuclease H: molecular diversities, substrate binding domains, and catalytic mechanism of the prokaryotic enzymes ppt

... 1493 MINIREVIEW Ribonuclease H: molecular diversities, substrate binding domains, and catalytic mechanism of the prokaryotic enzymes Takashi Tadokoro and Shigenori Kanaya Department of Material and Life Science, ... sites of these enzymes, these enzymes may also share a catalytic mechanism with RNase H. Substrate recognition mechanism of RNase...

Ngày tải lên: 30/03/2014, 02:20

... oligonucleotide, positioning the 3¢-end of the nascent DNA to act as a primer for the completion of minus strand synthesis. Step 3 The first template switch enables continued synthesis of the minus-strand DNA (Fig. ... located at both ends of the retroviral genome. The sequences complementary to PBS and R are denoted PBS¢ and R¢, respectively. The PPT serves as t...

Ngày tải lên: 30/03/2014, 02:20

... independently of the presence of AREs (Fig. 6B, lanes 2 and 5). It is possible that in the region located before the AREs, consisting of 343 bp of the 3¢ UTR of the IL-1b transcript, there is a putative ... MCPIP (endogenous and exogenous) starts degradation of IL-1b mRNA and also of its own tran- script. For this reason, the level of mRNA for the wild- typ...

Ngày tải lên: 18/02/2014, 14:20

... Biochemistry and Molecular Biology, University of Freiburg, Freiburg, Germany; 2 Laboratory of Biochemistry, University of Groningen, the Netherlands; 3 Institute of Organic Chemistry and Biochemistry, ... as the site of covalent attachment of FAD and confirmed Trp66 as essential for FAD binding, f or enzyme activity and for the spectral properties o f the wild-t...

Ngày tải lên: 19/02/2014, 16:20

... recorded, the user rece- ives feedback on the overall quality of the utter- ance. Specifically, the user receives feedback on the pitch, the overall amplitude, and the pronuncia- tion of the recording. ... smoother, more natural sounding synthesis, but requires many hours of recording, often by a trained professional. The recording process is usually supervised,...

Ngày tải lên: 20/02/2014, 09:20

... the set of case elements that modify the i-th verb in T rs i (T ) : the set of particles in the set of case el- ements that modify the i-th verb in T ns i (T ) : the set of nouns in the set of ... )) the co-occurrence prob- ability of the particle set and the verb, and we call P(n i,j (T )|r i,j (T ), v i (T )) the co-occurrence probability of the cas...

Ngày tải lên: 20/02/2014, 12:20

... that the po- sition probability is independent of the position of the Chinese word and the length of the Chinese sentence. And we assume that the position probability is in- dependent of the ... (2) ml, are the lengths of the source sentence and the target sentence respectively. j is the position index of the target word. j a is the position...

Ngày tải lên: 20/02/2014, 12:20

... present studies provide the first systematic and detailed characterization of the nucleotide binding specificity of the N- and C-terminal nucleotide binding sites of the 90-kDa molecular chaperone, ... specificities of Hsp90a and Hsp90b (data not shown). As an additional proof, we analyzed the competition of these nucleotides with ATP–Sepharose binding, in the...

Ngày tải lên: 08/03/2014, 02:21

... between TSC1 and TSC2, but results in a redistribution of the TSC1 ⁄ TSC2 protein complex from the membrane to the cytosol; and (c) alteration of the subcellular distribution of the TSC1 ⁄ TSC2 ... effect of IGF-1 stimulation on the functional interaction between TSC1 and TSC2, even though the phosphory- lation level of TSC2 was altered. The stability of the...

Ngày tải lên: 15/03/2014, 11:20

... 2, the wt ZIP1-Myc protein was mostly detec- ted in the perinuclear region of the CHO cells with a punctate distribution in the cytoplasm of the cell (Fig. 2A). The perinuclear staining of the ... System. The expression of either the wt or mutant ZIP1-Myc protein was normalized by the expression of GRP78. Values are the means ± SE, n ¼ 3. (C) Effects of cyclo...

Ngày tải lên: 16/03/2014, 11:20