Báo cáo khoa học: Structural framework of the GABARAP–calreticulin interface – implications for substrate binding to endoplasmic reticulum chaperones potx

... (2009) 114 0–1 152 ª 2009 The Authors Journal compilation ª 2009 FEBS Structural framework of the GABARAP–calreticulin interface – implications for substrate binding to endoplasmic reticulum chaperones Yvonne ... detailed view of the alignment is given in the bottom panels of Fig. 4 for the two hydrophobic pockets. Binding of Trp183 (dark bl...

Ngày tải lên: 30/03/2014, 02:20

... responsible, from a thermodynamic point of view, for the binding of the monomeric species of CAC? We have previously discussed the variation in the free energy of binding as a function of the changes ... movement of the molecule). Furthermore, the internal motions of each bond vector are independent of each other, but the rotational diffusion of the m...

Ngày tải lên: 07/03/2014, 06:20

... both chromatograms refer to the protein segments 6 0–6 3, 3 3–3 9, 6 4–7 8, 6 4–7 9, 8 4–9 0, 4 0–5 6, 5–3 2 + 7–3 2, 5–3 9 + 7–3 9. Peaks present in chromatogram A but not in B refer to segment 8 0–8 3. Peaks present ... both forms. The CD spectra of the 1–7 0 and 7 0–9 0 regions of the protein are reported in the inset of Fig. 1. The secondary...

Ngày tải lên: 07/03/2014, 14:20

... different substrates and the cata- lytic domain of the apo-enzyme. The binding of the small substrates in the active site leads to conformational changes in the motif 2 loop and SOL. The serine ... and the carbonyl oxygen of the serine substrate [12]. These movements are required to position the carboxylate oxygen for nucleophilic attack of the a-phosph...

Ngày tải lên: 16/03/2014, 06:20

... the secondary structure of the catalytic domain of PKCf and the effect of its substrate, MgATP, and also to study the effect of thermal unfolding in the presence and the absence of MgATP. We used 2D ... structure of 282 residues of a total of 344 have been solved [19]. The catalytic domain of PKCh possesses a high degree of homology ( 80%) with the cata...

Ngày tải lên: 16/03/2014, 13:20

... important clue for understanding the Nogo-40–NgR interaction. Recently, the deter mination of t he crystallographic s truc- ture of the N gR ectodomain l ed to the speculation that one potential Nogo-66 binding ... sufficient to stabilize a full helical conformation for the p eptide. NMR s pectroscopy was further utilized to explore the structural properties of...

Ngày tải lên: 16/03/2014, 16:20

... fimbriae or other appendages of the bacterium, a hypothesis requiring further investigation. It is of note that the structure of the LPS O-antigen of F. columnare differs structurally from the LPS ... to C6, 30 3–4 2, loss of ketene) confirmed that deuterium labeling was present. The chro- matographically isolated 2-amino-2,6-dideoxygalactose fraction was a mixture of...

Ngày tải lên: 17/03/2014, 10:20

... the association of the helices in the core of the oligomer, we calculated the electrostatic potential of the surfaces of the two long helical segments predicted from the above algo- rithms; these ... 38 8–3 97 ª 2005 The Authors Journal compilation ª 2005 FEBS to be helical in nature, implicating that these could be the main contributors to the core of t...

Ngày tải lên: 23/03/2014, 11:20

... used for general assignments of the CPS and LPS O-chain polysaccharide: COSY, TOCSY, NOESY and HSQC. Due to overlap in proton resonances, selective TOCSY and TOCSY–TOCSY experiments were used to complete ... 3 of residue b (Fig. 3B). Proton resonances for the CPS sample did not appear as resolved multiplets due to heterogeneity resulting from the presence of both the a...

Ngày tải lên: 23/03/2014, 13:20

... essential for the thermal stability a nd biological functions of the bacteria i n extreme environments [ 9–1 1]. The polar lipids found in Thermus aquaticus, Thermus filiformis, Thermus scotoductus,andThermus ... study is the first to determine the full structure of glycoglycerolipid in thermophilic bacteria Meiothermus spp. The structural information will be very useful...

Ngày tải lên: 23/03/2014, 13:20