... mL L )1 methanol, 100 mL L )1 acetic acid;Destain I: 500 mL L )1 methanol, 100 mL L )1 acetic acid;Destain II: 50 mL L )1 methanol, 70 mL L )1 acetic acid.ND9 6: < /b> 96 mmol L )1 NaCl, 2 mmol L )1 KCl, ... phosphorylation of < /b> recombinant, truncated, GIRK1 by the < /b> catalytic subunit of < /b> PKA. In the < /b> lower panels, the < /b> mean values and< /b> standard error of < /b> mean values of < /b> relative specific radioactivity of < /b> the < /b> different ... Ltd,Jerusalem, Israel); Protein A < /b> Sepharose (CL- 4B beads;Pharmacia LKB Biotechnology AB, Uppsala, Sweden);SpCAMPS, RpCAMPS (A1< /b> 66, A1< /b> 65, respectively; Sigma-Aldrich, St Louis, MO, USA); [32P]ATP[cP]...