Báo cáo khoa học: Helicobacter pylori single-stranded DNA binding protein – functional characterization and modulation of H pylori DnaB helicase activity pptx

... experiments with purified proteins, H. pylori (Hp) SSB bound specifically to ssDNA and modulated the enzymatic ATPase and helicase activity of HpDnaB helicase. HpSSB and HpDnaB proteins were co-localized ... report the cloning and functional characterization of the single-stranded DNA (ssDNA) binding protein from H. pylori. The N-ter- minal DNA binding doma...

Ngày tải lên: 30/03/2014, 02:20

... pH in the range 6. 3–7 .6, however, would suggest that the cavity at the protein DNA of the nonspecific complex and the more direct association of the recognition DNA and protein surfaces of the ... conditions of pH: pH 6.3 (m); pH 7.6 (j). The binding of the EcoRV proceeds in at least two steps. About 60% of the protein binds within the first 5 min of the kinetic...

Ngày tải lên: 22/03/2014, 16:20

... except for the insert (Fig. 7B). The TH activity of CfAFP-501 can be a s high as three times t hat o f isoform 33 7. Despite the higher activity than isoform 3 37, t he larger isoform l acks three Thr ... activity of sbwAFP [42]. The structure shows that there is a right- handed cap at the C-terminus of the protein, which forms two antiparallel sheets with b-stands from the prece...

Ngày tải lên: 19/02/2014, 16:20

... SG – ⁄ – MCs. However, the underlying mechanisms behind these observations were not defined. The aim of this study was therefore to provide insights into this issue by determining the fate of ... cathepsin E – ⁄ – MCs and that the storage of cathepsin E in MCs is dependent on heparin PG [13]. Thus, a likely explanation for the defective pro-CPA processing in SG – ⁄...

Ngày tải lên: 07/03/2014, 11:20

... fractions. The flowthrough contained the unbound material, which corresponds to single stranded DNA. The column was washed with 2.5 mL of 0.12 m phosphate buf- fer pH 6.8 and double-stranded DNA was ... Ehtbp [25] and Ehp53 [26] have been character- ized as the orthologous of the mammalian tbp and p53 genes, respectively. The E. histolytica TATA- binding protein (EhTBP) is the...

Ngày tải lên: 16/03/2014, 18:20

... the known crystal structure and 3D models of FA-metabolizing P450s in bacteria and animals, the authors confirmed HOOC HOOC OH HOOC O H HOOC COOH HOOC OH HOOC O -hydroxylation Epoxidation In chain ... synthesis. TEM of the epidermis showed that the structure of the cuticle was altered, which suggested that some of these phenotypes were due to a defect in the epidermal cuticle. It...

Ngày tải lên: 22/03/2014, 16:21

... analyzed the binding of HK and HKa to LM, FN and VN, and showed that both HK and HKa bind with high affinity to LM. The LMs used in the study comprised LM 1 and LM 10 ⁄ 11, the latter of which are characteristic ... with respect to the binding and function of HK and HKa. In the present study, we show that Zn 2+ is not required for the binding of either HK or HKa to...

Ngày tải lên: 30/03/2014, 01:20

... microscopy analysis of the FtsZ assembly reaction showed the formation of thicker and larger bundles of FtsZ proto- filaments in the presence of TMAO compared with the control (Fig. 6). The widths of the bundles ... commonly known as the solvophobic effect. Although TMAO reduced the fluorescence intensity of the FtsZ–ANS complex, it did not reduce the anisotropy of the FtsZ– ANS...

Ngày tải lên: 30/03/2014, 16:20

... region includes helices H3 (L69–L75) and H4 (K89–E114) and the linking coils (His63–Thr68 and Thr76–Lys83) that are apparently exposed in both the dodecameric and monomeric forms. These findings ... absorbance of those incubated with or without SOD. Each sample was assayed in triplicate [10]. Synthesis of H 2 N-His-His-His-His-His-His-OH Synthesis was performed by solid-phase m...

Ngày tải lên: 30/03/2014, 04:20

... we have to assume a very high binding constant of the protein for an extrane- ous ligand. We cannot state that the natural ligand of the H. pylori protein is erucamide, but the shape and size of ... 1899 of the protein cavity. The erucamide tail is deeply buried inside the protein cavity, which is fully hydrophobic, whereas the amidic head of the ligand is close to th...

Ngày tải lên: 16/02/2014, 14:20