Báo cáo khoa học: Helicobacter pylori single-stranded DNA binding protein – functional characterization and modulation of H pylori DnaB helicase activity pptx

Báo cáo khoa học: Helicobacter pylori single-stranded DNA binding protein – functional characterization and modulation of H. pylori DnaB helicase activity pptx

... experimentswith puriﬁed proteins, H. pylori (Hp) SSB bound speciﬁcally to ssDNA and modulated the enzymatic ATPase and helicase activity of HpDnaB helicase. HpSSB and HpDnaB proteins were co-localized ... report the cloning and functional characterization of the single-stranded DNA (ssDNA) binding protein from H. pylori. The N-ter-minal DNA binding domain shows signiﬁcant homology with E. coli single-stranded ... Sinha S, Kashav T, Mukhopadhyay G,Chaudhuri TK et al. (2007) The domain structure of Helicobacter pylori DnaB helicase: the N-terminaldomain can be dispensable for helicase activity whereasthe...

Báo cáo khoa học: Solution parameters modulating DNA binding speciﬁcity of the restriction endonuclease EcoRV docx

... pH in the range6. 3–7 .6, however, would suggest that the cavity at the protein DNA of the nonspeciﬁc complex and the moredirect association of the recognition DNA and protein surfaces of the ... conditions of pH: pH 6.3 (m); pH 7.6(j). The binding of the EcoRV proceeds in at least two steps.About 60% of the protein binds within the ﬁrst 5 min of the kineticexperiment. The time dependence of ... this study such that1 8–2 0 h incubation was enough to reach equilibrium(data not shown).EcoRV DNA speciﬁc binding measured with thegel mobility shift and self-cleavage assaysThe electrophoretic...

Tài liệu Báo cáo khoa học: Cold survival in freeze-intolerant insects The structure and function of b-helical antifreeze proteins pdf

... exceptfor the insert (Fig. 7B).The TH activity of CfAFP-501 can be a s high as threetimes t hat o f isoform 33 7. Despite the higher activity thanisoform 3 37, t he larger isoform l acks three Thr ... activity of sbwAFP [42]. The structure shows that there is a right-handed cap at the C-terminus of the protein, which formstwo antiparallel sheets with b-stands from the precedingcoil. The c onformation ... alignment of a single coil from each protein i s s hown inFig. 8B. TmAFP, with coils that are three residues s horterthan that of sbwAFP, has a m uch tighter coil path. Anothereffect of the tighter...

Báo cáo khoa học: A role for serglycin proteoglycan in granular retention and processing of mast cell secretory granule components ppt

... SG – ⁄ – MCs. However, the underlyingmechanisms behind these observations were notdeﬁned. The aim of this study was therefore to provideinsights into this issue by determining the fate of ... cathepsin E – ⁄ – MCs and that the storage of cathepsin E in MCs is dependenton heparin PG [13]. Thus, a likely explanation for thedefective pro-CPA processing in SG – ⁄ – MCs is that thelack ... process is associatedwith both increased total synthesis of sulfated PGs and increased charge density of the synthesized PGs.DiscussionAlthough the knockout of both SG [10] and N-de-acetylase...

Báo cáo khoa học: Entamoeba histolytica TATA-box binding protein binds to different TATA variants in vitro ppt

... fractions. The ﬂowthrough contained the unboundmaterial, which corresponds to single stranded DNA. Thecolumn was washed with 2.5 mL of 0.12 m phosphate buf-fer pH 6.8 and double-stranded DNA was ... Ehtbp [25] and Ehp53 [26] have been character-ized as the orthologous of the mammalian tbp and p53 genes, respectively. The E. histolytica TATA- binding protein (EhTBP) is the only member of ... only member of thebasal transcription machinery cloned and character-ized in this parasite.The EhTBP functional DNA- binding domain has55% homology with human TBP, whereas theEhTBP N-terminal...

Báo cáo khoa học: Cytochrome P450 metabolizing fatty acids in plants: characterization and physiological roles pptx

... the knowncrystal structure and 3D models of FA-metabolizingP450s in bacteria and animals, the authors conﬁrmedHOOCHOOCOHHOOCO H HOOCCOOHHOOCOHHOOCO-hydroxylationEpoxidationIn chain ... synthesis. TEM of the epidermisshowed that the structure of the cuticle was altered,which suggested that some of these phenotypes weredue to a defect in the epidermal cuticle. It was thusproposed ... mostly for the synthesis of C16–C18 and C22–C24 x-hydroxy FAs respectively, in plantsco-overexpression of these enzymes with the GPAT5acyltransferase of suberin synthesis shows that theirspeciﬁcity...

Báo cáo khoa học: High molecular weight kininogen binds to laminin – characterization and kinetic analysis pot

... analyzed the binding of HK and HKa toLM, FN and VN, and showed that both HK and HKa bind with high afﬁnity to LM. The LMs used inthe study comprised LM 1 and LM 10 ⁄ 11, the latter of which are characteristic ... withrespect to the binding and function of HK and HKa.In the present study, we show that Zn2+is notrequired for the binding of either HK or HKa to LM.However, it enhanced the afﬁnity of the ... interaction of the kini-nogens with the proteins in the basal membrane, whichis of equal importance when explaining the in vivoeffect of HKa. Therefore, the binding of HK and HKato selected...

Báo cáo khoa học: A natural osmolyte trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea docx

... microscopyanalysis of the FtsZ assembly reaction showed theformation of thicker and larger bundles of FtsZ proto-ﬁlaments in the presence of TMAO compared with thecontrol (Fig. 6). The widths of the bundles ... commonlyknown as the solvophobic effect. Although TMAOreduced the ﬂuorescence intensity of the FtsZ–ANScomplex, it did not reduce the anisotropy of the FtsZ–ANS complex. Thus, the reduction in the ﬂuorescenceintensity ... concentra-tions and synthetic pathways of trimethylamine oxide and urea in some teleost ﬁshes of McMurdo Sound,Antarctica. Fish Physiol Biochem 18, 38 7–3 98.11 Yancey PH & Siebenaller JF (1999) Trimethylamineoxide...

Báo cáo khoa học: Helicobacter pylori neutrophil-activating protein activates neutrophils by its C-terminal region even without dodecamer formation, which is a prerequisite for DNA protection – novel approaches against Helicobacter pylori inﬂammation ppt

... region includeshelices H3 (L69–L75) and H4 (K89–E114) and thelinking coils (His63–Thr68 and Thr76–Lys83) that areapparently exposed in both the dodecameric and monomeric forms.These ﬁndings ... absorbance of thoseincubated with or without SOD. Each sample was assayedin triplicate [10].Synthesis of H 2N-His-His-His-His-His-His-OHSynthesis was performed by solid-phase methodology on ... plasmid with the ribosomal protein S14 fromThermus thermophilus pTZ-S14 DNA (20 nm). The bufferswere 20 mm phosphate and 50 mm NaCl (pH 6.5), and 20 mm Hepes and 50 mm NaCl (pH 7.5). The HP-NAPwas...

Tài liệu Báo cáo khoa học: Helicobacter pylori acidic stress response factor HP1286 is a YceI homolog with new binding speciﬁcity pdf

... we have to assume a veryhigh binding constant of the protein for an extrane-ous ligand.We cannot state that the natural ligand of the H. pylori protein is erucamide, but the shape and size of ... 1899 of the protein cavity. The erucamide tail is deeply buriedinside the protein cavity, which is fully hydrophobic,whereas the amidic head of the ligand is close to theopen end of the cavity, ... in an Escherichia coli heterolo-gous system. The shape and length of the cavity indicate that, at variancewith other members of the family, HP-YceI has a binding speciﬁcity foramphiphilic compounds...

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