Báo cáo khoa học: Conformational stability of neuroglobin helix F – possible effects on the folding pathway within the globin family potx

... 2009 The Authors Journal compilation ª 2009 FEBS Conformational stability of neuroglobin helix F – possible effects on the folding pathway within the globin family Luca Codutti 1, *, Paola Picotti 2, * , , ... variance from helix F in apoMb. These results allowed us to infer that the folding pathway of apoNgb is different from that of apoMb...

Ngày tải lên: 30/03/2014, 01:20

... 17b-HSDcl at 25 °C The effects of the coenzyme NADPH on the confor- mation of 17b-HSDcl were investigated at pH 7.3 in NaCl ⁄ P i buffer. The influence of NADPH on the far- UV CD spectrum of 17b-HSDcl ... as results of the oligomerization processes. The presence of high concentrations of salt has two effects on protein–protein interactions: First, the pr...

Ngày tải lên: 16/03/2014, 13:20

... the fraction of functional HbA after the temperature cycle was only 20%. The shape of thermal denaturation curves of the various globins may differ, suggesting different mecha- nisms or degrees of cooperativity ... of the kinetics was performed by numerical integration of the differential equations for the transition between the three species: (penta-coordinated) F...

Ngày tải lên: 30/03/2014, 16:20

... degradation of the Polo kinase Cdc5 promotes the return of Cdc14 into the nucleolus. Genes Dev 22, 7 9–9 0. 55 Sullivan M & Uhlmann F (2003) A non-proteolytic function of separase links the onset of ... determining the role of CDKs in the regulation of mitotic Clb cyclin degradation, by examining the consequences of CDK inactivation on mitotic Clb cyclin...

Ngày tải lên: 23/03/2014, 06:20

... protonation of the distal His64 in Ngb is responsible for the pH-depen- dent changes in the oxygen affinity of this protein [10]. Discussion Conformational stability of Ngb In this study, the conformational ... thermophilic Aquifex aeolicus [54]. Functional stability of Ngb The conformational stability of the apoNgb structure in acid is in agreement with the...

Ngày tải lên: 07/03/2014, 02:20

... that the formation of oligomers might be reversible. To characterize the properties of O n >2 induced by low concentrations of denaturants, we investigated the effect of NaCl on the formation of ... not only the fold- ing of the individual domains, but also the organiza- tion of these domains [24], and the complexity may result in different descriptions of...

Ngày tải lên: 23/03/2014, 04:21

... to the removal of free fatty acid suggested that bLG could facilitate the digestion of milk fat [16]. The main goal of the present investigation was to obtain information on the conformation of ... medium for ion diffusion and ion pair formation, which leads to the movement of the charged groups of the protein in order to keep themselves neutral. As a means of...

Ngày tải lên: 07/03/2014, 15:20

... h, of the tryptophans attached in the protein backbone may be calculated from the amplitude b 1 of the fast motion [7]: 1 À b fast r o ¼ 3 cos 2 h À 1 2 ð1Þ The average cones of rotation of the ... collected at an angle of 90° with respect to the direction of the excitation light beam. Extreme care was taken to avoid artefacts from depolarization effects. At the...

Ngày tải lên: 07/03/2014, 21:20

... substitutions in KKCC and KCCC affected the binding kinetics, the thermal stability of Hsp70–peptide complexes, and the refolding activity which might reflect a destabilization of the PBD. Conformational ... Characterization of a lidless form of the mole- cular chaperone DnaK: deletion of the lid increases peptide on- and off-rate constants. J Biol Chem 276, 2723 1–...

Ngày tải lên: 16/03/2014, 22:20

... additional T170C mutation, the pK a of M accumulation is raised as shown in Fig. 8B. The reason is that the M–N trans- ition is coupled to deprotonation of D96 and protona- tion of the Schiff base. ... revolu- tionized by the production of a family of high resolu- tion X-ray diffraction structures [3,4]. The structures and structural changes assigned to the intermedi...

Ngày tải lên: 23/03/2014, 13:20