Báo cáo khoa học: Functional analysis of pyrimidine biosynthesis enzymes using the anticancer drug 5-fluorouracil in Caenorhabditis elegans docx

... and 5-FU. Uncovering the mechanism of 5-FU resistance and characterization of pyrimidine biosynthesis enzymes in C. elegans will help to further our under- standing of pyrimidine biosynthesis enzymes ... Authors Journal compilation ª 2009 FEBS Functional analysis of pyrimidine biosynthesis enzymes using the anticancer drug 5-fluorouracil in Caen...

Ngày tải lên: 30/03/2014, 01:20

... compared with other DtxR-like proteins (Fig. 2A). The high conservation of these domains agrees with the important role of domain 1 on DNAÆprotein interaction and of domain 2 in the protein dimerization ... such as: DNA-binding ferritin- like protein, ferritins and bacterioferritins Putative dpsA gene The Dps protein family includes DNA-binding hemoproteins in several bacter...

Ngày tải lên: 16/03/2014, 18:20

... C-terminal region, including the PAS-B domain of BMAL1, is required for the binding to DEC1, Fig. 4. Identification of the domain of D EC1 that interacts with BMAL 1. Interactions of various DEC1 ... region of DEC1, including the bHLH domain, interacted with the C-terminal r egion o f BMAL1 in a mammalian two-hybrid assay. Accordingly, a Fig. 5. Analysis of DEC1 mutan...

Ngày tải lên: 19/02/2014, 16:20

... the effect of the proximities of the antibody-binding and Fn-binding sites, the interaction of the repeat with Fn may be sterically hindered in the presence of the anti- body. The results of this ... to the N-terminal segment and the fibronectin-binding site to the C-terminal segment of the repeat. The distinct localization of the 15E11 epitope and...

Ngày tải lên: 06/03/2014, 22:21

... structures formed in the presence of misfolded proteins and con- tain components of the protein degradation and refold- ing machinery, including proteasome complexes and members of the Hsc70 and ... measuring the intensity of the individual GALE and tubulin bands in the western blot using Scion Image (Scion Corporation, Frederick, MD, USA). Generation of stable cel...

Ngày tải lên: 07/03/2014, 00:20

... substrate binding sites. The data represents the first step in the analysis of the metabolic control of flux through the Leloir pathway. Analysis of the galactokinase, its mutants, and the other enzymes of ... in these proteins. The results from the soluble mutants support the hypothesis that motif I interacts with galactose and that motif V plays a role i...

Ngày tải lên: 08/03/2014, 02:20

... anal- ysis of plant b-glucosidases to obtain insights into the evolution of the molecular sites involved in the two modes of aglycone binding. The amino acid sequence of Zm-p60.1 was com- pared with the ... distances of reacting atoms. The only noted difference was in the orienta- tion of the aromatic ring of pNPGlc in the F193A mutant (Fig. 6D), owing to...

Ngày tải lên: 16/03/2014, 04:20

... loops. In addition, GPCRs contain a more or less extended N-terminal domain and C-terminal domain, which are both often involved either in ligand binding or G-pro- tein coupling. Owing to their ... and the quality of the sample is very similar to that of the related rhodopsin isolated from natural sources. The binding of human endothelin B receptor to its natural peptide lig...

Ngày tải lên: 16/03/2014, 10:20

... during purification. Kinetic analysis of disease-causing mutations The steady-state kinetic parameters of each of the nine disease-causing mutant proteins were determined (Table 1). In general, little ... EcoRI at the 3¢ end) and to encode a hexa-histidine tag at the N terminus of the expressed protein. PCR products were cut with these enzymes and inserted into the co...

Ngày tải lên: 23/03/2014, 11:20

... strategy involved the insertion of several fragments of the 5¢-region of mce-1 into the mul- tiple cloning site of the vector pPD95.77 provided by A. Fire (Carnegie Institute, Baltimore, MD, USA). The ... cleft, with the first strand of the N-terminal module antiparallel to the first strand of the C-terminal module. At the bot- tom of this U-shaped cavity is t...

Ngày tải lên: 23/03/2014, 13:20