Báo cáo khoa học: Functional analysis of pyrimidine biosynthesis enzymes using the anticancer drug 5-ﬂuorouracil in Caenorhabditis elegans docx

... and5-FU. Uncovering the mechanism of 5-FU resistanceand characterization of pyrimidine biosynthesis enzymes in C. elegans will help to further our under-standing of pyrimidine biosynthesis enzymes ... Authors Journal compilation ª 2009 FEBS Functional analysis of pyrimidine biosynthesis enzymes using the anticancer drug 5-ﬂuorouracil in Caenorhabditis elegans Seongseop Kim1,*, Dae-Hun Park2,*, ... To study the pyrimidine biosynthesis path-way in Caenorhabditis elegans, we screened for mutants exhibiting resis-tance to the anticancer drug 5-ﬂuorouracil (5-FU). In several strains,mutations...

Báo cáo khoa học: Functional analysis of two divalent metal-dependent regulatory genes dmdR1 and dmdR2 in Streptomyces coelicolor and proteome changes in deletion mutants ppt

... compared with other DtxR-like proteins(Fig. 2A). The high conservation of these domainsagrees with the important role of domain 1 onDNAÆprotein interaction and of domain 2 in the proteindimerization ... such as: DNA-binding ferritin-like protein, ferritins and bacterioferritinsPutative dpsA gene The Dps protein family includes DNA-binding hemoproteins in several bacteriaP3Medium increasesco6501 ... and the knownmembers of this group; namely DmdR2 contains aPro- and Ala-rich stretch of eight amino acid residuesat the beginning of domain 3, which is absent in the other DmdR proteins.The...

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... C-terminal region, including the PAS-Bdomain of BMAL1, is required for the binding to DEC1,Fig. 4. Identiﬁcation of the domain of D EC1that interacts with BMAL 1. Interactions of various DEC1 ... region of DEC1, including the bHLH domain, interacted with the C-terminal r egion o fBMAL1 in a mammalian two-hybrid assay. Accordingly, aFig. 5. Analysis of DEC1 mutants for bindingto the CACGTG ... with BMAL1 and the bin ding to the E-box.Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box.However, substitution of His57 for Ala...

Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the ﬁbronectin-binding adhesins ﬁbronectin-binding protein A and ﬁbronectin-binding protein B from Staphylococcus aureus pot

... the effect of the proximities of the antibody-binding andFn-binding sites, the interaction of the repeat with Fnmay be sterically hindered in the presence of the anti-body. The results of this ... to the N-terminal segment and the ﬁbronectin-binding siteto the C-terminal segment of the repeat. The distinct localization of the 15E11 epitope and the ﬁbronectin-binding site suggests that the ... ﬁbronectin. The C-terminal domain of ﬁbronectin-binding protein A is organized into 11tandem repeats, six of which bind the ligand with high afﬁnity; other sitesbind more weakly. Fibronectin-binding...

Báo cáo khoa học: Functional analysis of mutations in UDP-galactose-4epimerase (GALE) associated with galactosemia in Korean patients using mammalian GALE-null cells pdf

... structuresformed in the presence of misfolded proteins and con-tain components of the protein degradation and refold-ing machinery, including proteasome complexes andmembers of the Hsc70 and ... measuring the intensity of the individual GALE and tubulin bands in the western blot using Scion Image (Scion Corporation,Frederick, MD, USA).Generation of stable cell lines and BrdUincorporation ... tested the stability of the mutant proteins bydetermining protein half-life using cycloheximide.Wild-type GALE produced stable protein with a half-life of > 8 h. Unlike that of wild-type, the...

Báo cáo khoa học: Functional analysis of disease-causing mutations in human galactokinase potx

... substratebinding sites. The data represents the ﬁrst step in the analysis of the metabolic control of ﬂux through the Leloirpathway. Analysis of the galactokinase, its mutants, and the other enzymes of ... in these proteins. The results from the soluble mutants support the hypothesis thatmotif I interacts with galactose and that motif V plays arole in maintaining the structural integrity of the ... group of GHMP kinases in which proton transfer does not play amajor role in the rate determining step of catalysis.That ﬁve of the 10 disease-causing mutations resulted in insoluble protein in...

Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot

... anal-ysis of plant b-glucosidases to obtain insights into the evolution of the molecular sites involved in the twomodes of aglycone binding. The amino acid sequence of Zm-p60.1 was com-pared with the ... distances of reactingatoms. The only noted difference was in the orienta-tion of the aromatic ring of pNPGlc in the F193Amutant (Fig. 6D), owing to lost van der Waals contactwith the side-chain of ... calculated using origin pro 7.5software (OriginLab Corp., Northampton, MA, USA). The concentrations of the proteins in the preparations weredetermined using the DC Protein Assay (Bio-Rad) withBSA...

Báo cáo khoa học: Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation pptx

... loops. In addition, GPCRs contain a more or less extendedN-terminal domain and C-terminal domain, which areboth often involved either in ligand binding or G-pro-tein coupling.Owing to their ... and the quality of the sample is verysimilar to that of the related rhodopsin isolated from natural sources. The binding of human endothelin B receptor to its natural peptide ligand endo-thelin-1 ... produced either as a soluble protein in the presence of detergents or as a precipitate in the absence of detergents. We analyzed the yield andsample quality of ETB synthesized in the presence of Ligand...

Báo cáo khoa học: Functional analysis of disease-causing mutations in human UDP-galactose 4-epimerase pot

... during puriﬁcation.Kinetic analysis of disease-causing mutations The steady-state kinetic parameters of each of the ninedisease-causing mutant proteins were determined(Table 1). In general, little ... EcoRI at the 3¢end) and to encode a hexa-histidine tag at the N terminus of the expressed protein. PCR products were cut with these enzymes and inserted into the corresponding sites in the expression ... be the case withmost of the mutations in GALE.Although all the mutant proteins were active, some of their kinetic properties differed from that of the wild-type protein and from each other. The...

Báo cáo khoa học: Functional analysis of the methylmalonyl-CoA epimerase from Caenorhabditis elegans docx

... strategy involved the insertion of several fragments of the 5¢-region of mce-1 into the mul-tiple cloning site of the vector pPD95.77 provided by A.Fire (Carnegie Institute, Baltimore, MD, USA). The ... cleft, with the ﬁrst strand of the N-terminal module antiparallel to the ﬁrst strand of the C-terminal module. At the bot-tom of this U-shaped cavity is the metal binding site,where the divalent ... of the domains occurs in several differentways, suggesting that the evolution of these proteinsprobably involved multiple gene duplication, genefusion and domain swapping events. Members of...

Xem thêm