0
  1. Trang chủ >
  2. Luận Văn - Báo Cáo >
  3. Báo cáo khoa học >

Báo cáo khoa học: The interaction of the Escherichia coli protein SlyD with nickel ions illuminates the mechanism of regulation of its peptidyl-prolyl isomerase activity ppt

Báo cáo khoa học: The interaction of the Escherichia coli protein SlyD with nickel ions illuminates the mechanism of regulation of its peptidyl-prolyl isomerase activity ppt

Báo cáo khoa học: The interaction of the Escherichia coli protein SlyD with nickel ions illuminates the mechanism of regulation of its peptidyl-prolyl isomerase activity ppt

... of the Escherichia coli protein SlyD with nickel ions illuminates the mechanism of regulation of its peptidyl-prolyl isomerase activity Luigi Martino1,2, Yangzi He1,*, Katherine L. D. Hands-Taylor1, ... PPIase site of SlyD. Although coher-ent with the fact that SlyD appears to interact in the proximity of the nickel ion high-affinity motif of HypB, the exact mechanism of the SlyD HypB interaction remains ... interactionsbetween the nickel ion and the protein is the keyfeature of the binding process.Further evidence that SlyD interacts with nickel ions with a 1 : 1 stoichiometry is provided by the analysis of the...
  • 16
  • 233
  • 0
Báo cáo khoa học: A single mutation in Escherichia coli ribonuclease II inactivates the enzyme without affecting RNA binding pot

Báo cáo khoa học: A single mutation in Escherichia coli ribonuclease II inactivates the enzyme without affecting RNA binding pot

... after 2 h of IPTGtreatment, and these were the conditions used for fur-ther purification of the protein. The exoribonucleolytic activity of the crude extracts,before and after induction with IPTG, ... stalling of the enzyme in the vicinity of the two secondary structures of the mRNA.Metal dependency of the exoribonuclease activity of RNase II The above results pointed out the importance of Asp209 ... highly active in the degra-dation of poly[8-3H]adenylic acid, with an activity of > 325 UEÆlg)1 of protein (results not shown) (UE:units of enzymatic activity, namely the amount of pro-tein...
  • 12
  • 320
  • 0
Báo cáo khoa học: Are UV-induced nonculturable Escherichia coli K-12 cells alive or dead? ppt

Báo cáo khoa học: Are UV-induced nonculturable Escherichia coli K-12 cells alive or dead? ppt

... interpretation of the state of cells is often ambiguous [10,11]. Problems in the interpretation of the state of cells that have lostculturability are due, not only to the absence of consensuson the definition ... elongation, protein synthesis and glucose meta-bolism was analysed, as well as the effect of differentaeration conditions on the regulation of metabolic fluxesthrough the Embden–Meyerhof and pentose ... depend on the activity of the PP pathway. In fact, when [1-13C]glucose is used as the carbon source, part of the 13C label is lost as CO2in the phosphogluconate dehydrogenase step of the PP...
  • 7
  • 318
  • 0
Báo cáo khoa học: Cholesterol interaction with the related steroidogenic acute regulatory lipid-transfer (START) domains of StAR (STARD1) and MLN64 (STARD3) ppt

Báo cáo khoa học: Cholesterol interaction with the related steroidogenic acute regulatory lipid-transfer (START) domains of StAR (STARD1) and MLN64 (STARD3) ppt

... or thermal degradation [37].To test whether this occurs in the case of the STARTproteins, we analyzed the migration behavior of theseproteins in SDS gels under various conditions.First, the ... the Coomassie stains of the purified proteins. The apparent molecular masses of the His-tag START proteins in the SDS ⁄ PAGE system wereslightly greater than the calculated molecular masses of 25 ... °C. The protein was cleaved by CNBr and further processed as described in the legend for Fig. 3. The asterisks mark the position of the 6.2 kDa band. The reference lines (dotted) correspond to the...
  • 13
  • 404
  • 0
Báo cáo khoa học: SHIP2 interaction with the cytoskeletal protein Vinexin ppt

Báo cáo khoa học: SHIP2 interaction with the cytoskeletal protein Vinexin ppt

... Transfection of vector alone does notmodify the percentage of attached cells. Therefore, the control of SHIP2 (or his mutants) transfected cellscould be the result of either the suggested conditions with ... associated with the cell–matrix adhe-sion sites in a network of protein- like integrins andF-actin [35]. Our hypothesis is that the interaction of SHIP2 with Vinexin (and perhaps other non enzymaticcomponents ... agreement with previous results obtained with CAPin stimulated COS-7 or CHO-IR cells [20]. Therefore, the interaction of Vinexin with the proline-rich domain of SHIP2 is not influenced by its tyrosine...
  • 15
  • 305
  • 0
Báo cáo khoa học: Mitochondrial transcription factor A overexpression and base excision repair deficiency in the inner ear of rats with D-galactose-induced aging pdf

Báo cáo khoa học: Mitochondrial transcription factor A overexpression and base excision repair deficiency in the inner ear of rats with D-galactose-induced aging pdf

... the com-mon deletion. By applying the specific probe, we caneven detect the presence of the common deletion in the inner ear in the control group. The proportions of the common deletion in the ... representativeresults for relative abundance of the protein tested. Ascompared with the control group, the expression of TFAM protein in the inner ear of the low-dose, med-ium-dose and high-dose ... induced byD-GalTo further understand the expression of TFAM protein in the inner ear, western blot analysis was performed. The protein levels of TFAM in the inner ear of d-Gal-treated rats...
  • 11
  • 450
  • 0
Báo cáo khoa học: Carbohydrate binding sites in Candida albicans exo-b-1,3-glucanase and the role of the Phe-Phe ‘clamp’ at the active site entrance ppt

Báo cáo khoa học: Carbohydrate binding sites in Candida albicans exo-b-1,3-glucanase and the role of the Phe-Phe ‘clamp’ at the active site entrance ppt

... solved the crystal structure of this GH5enzyme in the presence of two different mechanism- based inhibitors, thereby revealing the close network of interactions that hold the terminal glucose of the ... help explain their importance to the specificity andcatalytic efficiency of the enzyme. The role of the Phe-Phe entranceway The fortuitous discovery of an unreacted molecule of the mechanism- based ... to these conserved interactions at the )1 site,aromatic side chains further from the catalytic centreinteract with individual glucopyranoside units throughstacking interactions, as seen in the...
  • 13
  • 498
  • 0
Báo cáo khoa học: Metal exchange in metallothioneins – a novel structurally significant Cd5 species in the alpha domain of human metallothionein 1a ppt

Báo cáo khoa học: Metal exchange in metallothioneins – a novel structurally significant Cd5 species in the alpha domain of human metallothionein 1a ppt

... to pH 2.8. Elution of the protein using aneluant of low pH effectively removes the metal ions from the protein, which are then separated from the protein bandas a result of the size-exclusion ... the coordination of the fifth Cd2+ion by thiolate ligands within the clus-ter, and, more specifically, within the crevice site of the protein. The biological significance of the Cd5a species ... to remove the bulk of the oxygen from the solu-tions in order to deter oxidation of the protein. Cd2+was added incrementally to the solution of Zn4a to8.2 molar equivalents, with thorough...
  • 13
  • 438
  • 0
Báo cáo khóa học: S-(2,3-Dichlorotriazinyl)glutathione A new affinity label for probing the structure and function of glutathione transferases potx

Báo cáo khóa học: S-(2,3-Dichlorotriazinyl)glutathione A new affinity label for probing the structure and function of glutathione transferases potx

... all stages of the reaction. The best explanation for these results maybe that the reaction of SDTG at the binding site of onesubunit changes the conformation of the o ther subunit,thereby completely ... the dynamics of this enzyme. A plot of the crystallographic B-factors along the polypeptide chain can give an indication of the relative flexibility of the different portions of the protein (Fig. ... at the end of a-helix H¢¢¢3and formspart of the xenobiotic-binding site [11]. A lthough the thioether bond of methionine is usually considered to be of low reactivity, a number of pieces of...
  • 9
  • 556
  • 0
Báo cáo khoa học: Human ATP-dependent RNA ⁄ DNA helicase hSuv3p interacts with the cofactor of survivin HBXIP ppt

Báo cáo khoa học: Human ATP-dependent RNA ⁄ DNA helicase hSuv3p interacts with the cofactor of survivin HBXIP ppt

... the hSuv3pdomain that is necessary for interaction with HBXIP. (B) In vitro interaction of hSuv3p with HBXIP. The SDS ⁄ PAGE analysis of the binding of wildtype hSuv3p or its mutant devoid of ... in the truncated protein levelswithin 4 h following inhibition of protein synthesis bycycloheximide. We next wanted to examine whether the presence of HBXIP binding protein fragment of the hSuv3 ... assay the purity of the sample. Inaddition, the purified proteins were immunobloted andprobed with PAP antibodies (Sigma, Steinheim, Germany).In vitro protein protein interaction The in vitro interaction...
  • 12
  • 468
  • 0

Xem thêm

Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnBáo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Nghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíChuong 2 nhận dạng rui roTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)chuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)Chiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015