Báo cáo khoa học: Engineering of monomeric FK506-binding protein 22 with peptidyl prolyl cis-trans isomerase Importance of a V-shaped dimeric structure for binding to protein substrate docx

... crystallized forms of Torpedo californica, human and mouse AChEs. According to this alignment, the X- ray structure of Torpedo californica AChE should provid e a good probe model to solve the structure ... by anion-exchange and af®nity chromatography. Axelsen et al. reported that decamethonium, used during the last af®nity chromatogra- phy step of T. californica AChE, was pres...

Ngày tải lên: 17/03/2014, 11:20

... 5¢-AGAGAGAATT CATATGTCAGATT TGTTCAG-3¢ for primer 1; 5¢-TTCCATACCACCACCT GCAACTTGAAGCTC-3¢ for primer 2; 5¢-GTTGCAGGT GGTGGTATGGAACAGCATGCT-3¢ for primer 3; and 5¢-GGCCACT GGATCCAACTACAGCAATTCTCA-3¢ for primer ... Angkawidjaja 1 , Yuichi Koga 1 , Kazufumi Takano 1,2 and Shigenori Kanaya 1 1 Department of Material and Life Science, Graduate School of Engineering, Osaka University,...

Ngày tải lên: 30/03/2014, 01:20

... was used to transform QC774 cells, and this transformant was plated onto a 1 lm paraquat plate so as to obtain approximately 4000 transformants. The growth (or lack of growth) of these Q14 3A ... were plated again on 1 lm paraquat agar plates, so as to obtain approximately 500 colonies per plate. After incubation of these replated mutant colonies at 37 °C for another 20–24 h,...

Ngày tải lên: 07/03/2014, 11:20

... reaction were the 5¢-ATGGAACGATGGTTTAAATCTCTG-3¢ and 5¢-ATG TTTAACGCATTATTCGTTGTTGTTTTTG-3¢, and the reverse primers were the 5¢ -TCAATAGGTGTGGAAATA GTCCTGG-3¢ and 5¢-TTAAGCTTTTAATAAGCGTGG AAGTAATCC-3¢. ... inactivation of the mutant Asp133Val and its saturation variants. The residual activities of the mutant Asp133Val (e) and of the saturation variants were measured at var- ious times...

Ngày tải lên: 30/03/2014, 02:20

... albeit with F,W angles far from the allowed Ramachandran region, are virtually identical to that of FVIIa. Model- ling of Ala372 (223 ) into FVIIa showed that the Cb atom clashed with that of Arg315(170C), ... processed at a reduced rate by G37 2A- FVIIa, and K m for S -228 8 was increased by a factor of four (Fig. 1A) . Results obtained with S-2366 also revealed a red...

Ngày tải lên: 18/02/2014, 08:20

... mori: characterization and immunohistochemistry. Mol Cell Endocrinol 51, 227 – 235. 41 Nagata K, Maruyama K, Nagasawa H, Urushibata I, Isogai A, Ishizaki H & Suzuki A (1992) Bombyxin-II and its ... HF & Grunert LW (2002) Bombyxin is a growth factor for wing imaginal disks in Lepidoptera. Proc Natl Acad Sci USA 99, 15446–15450. 30 Satake S, Masumura M, Ishizaki H, Nagata K, Kat...

Ngày tải lên: 18/02/2014, 13:20

... further decrease the stability of the canavany- lated species. The role of tRNA as a cofactor for aminoacylation in those aminoacyl-tRNA synthetases that require tRNA for amino acid activation is ... substrate of 1.3 mm was determined, and the relative magnitude of the k cat ⁄ K M parameters for arginine and canavanine charging revealed a discrimination factor of 485; a...

Ngày tải lên: 18/02/2014, 13:20

... genes: elongation factor 2 (EF-2), 5¢-GACATCACCAAGGGTGTGCA-3¢ (forward) and 5¢-TTCAGCACACTGGCATAGAGGC-3¢ (reverse); GAPDH,5¢-CCGAGCCACATCGCTCAGAC-3¢ (forward) and 5¢-GTTGAGGTCAATGAAGGGGTC-3¢ (reverse). ... and functional properties of RNase and participates in degradation of IL-1b mRNA Danuta Mizgalska 1 , Paulina We˛ grzyn 1 , Krzysztof Murzyn 2 , Aneta Kasza 1 , Aleksander Koj 1 , Jacek...

Ngày tải lên: 18/02/2014, 14:20

... GGAAGTCTCCCTTCCCAGAC CGATTTGCTGACCACCTTCT MLL1 GAGGACCCCGGATTAAACAT GGAGCAAGAGGTTCAGCATC MLL2 GTGCAGCAGAAGATGGTGAA GCACAATGCTGTCAGGAGAA MLL3 AAGCAAACGGACTCAGAGGA ACAAGCCATAGGAGGTGGTG MLL4 GTCTATGCGCAGTGGAGACA ... TGCCCTCATATAAACCTGGAA AGCCTTTGGGAGTAGGAACC ERa antisense CATGGTCATGGTCAG a ERb antisense GAATGTCATAGCTGA a MLL1 antisense TGCCAGTCGTTCCTCTCCAC a MLL2 antisense ACTCTGCCACTTCCCG...

Ngày tải lên: 18/02/2014, 14:20

... to the mean nuclear DNA (nDNA) value for a given extract (mtDNA ⁄ nDNA). Furthermore, these values were expressed as a percentage related to the value resulting from the designated calibrator sample ... second-derivative maximum of each amplification reaction and relating it to its respective stand- ard curve. The results from the quantitative PCR were expressed as the ratio of...

Ngày tải lên: 20/02/2014, 01:20