Báo cáo khoa học: Crystal structures of open and closed forms of cyclo⁄maltodextrin-binding protein pptx

... resemble the open and closed forms, respectively, of other Fold- type II PLP-dependent enzymes. The solvent accessible surface areas of StDSD in the open and closed forms are 16 994 and 16 540 ... Bharath et al. Crystal structure of D-serine deaminase FEBS Journal 278 (2011) 2879–2891 ª 2011 The Authors Journal compilation ª 2011 FEBS 2891 Crystal structures of...

Ngày tải lên: 28/03/2014, 22:20

... open form. Results and Discussion Overall structures of TvuCMBP–ligand complexes The crystal structures of TvuCMBP liganded with a-CD, b-CD and G4 have been determined at 1.7, 1.8 and 1.55 A ˚ resolutions, ... Asp83 and Glu129 form hydrogen bonds with different atoms, O2 of Glc-2(CD), O3 of Glc-3(CD), and O3 of A B Fig. 4. Stereo views of N-domains involved in li...

Ngày tải lên: 29/03/2014, 23:20

... thermostability of TtAK. Comparison of the crystal structures of TtAKb and CgAKb was expected to elucidate the mechanism of the elevated thermostability of TtAKb. In this article, we describe the crystal structures ... denaturation temperature (T m )of4 0°C. Comparison of the crystal structures of TtAKb and the regulatory subunit of C. glutamicum AK showed tha...

Ngày tải lên: 18/02/2014, 08:20

... These structures provide insight into the key features of PDH specificity and mode of inhibition of the enzyme. Results and Discussion Structural analysis and model quality Crystal structures of ... Catalytic reaction of PDH and two intermediate states. (B) Structures and numbering of the inhibitors PEX (two resonance forms) and PEA. 6-Phosphogluconate dehydrog...

Ngày tải lên: 19/02/2014, 05:20

... the b-propeller and b-sandwich domains involve contacts of IV and V blades and b-strands (1–6) from the internal b-sheet of the b-sand- wich domain. The last four amino acids of the native sequence ... the native and recombinant protein were 58 091 and 59 156 Da, respectively. The results of MS of recombi- nant b-fructofuranosidase are close to the theoretical value of...

Ngày tải lên: 06/03/2014, 00:21

... constructs of both NiV and HeV F proteins. A schematic diagram of NiV and HeV F proteins with the location of structurally significant domains is given. The listed sequences of HR1 (137–178) and HR2 ... effects of HR2 on viral fusion and formation of the fusion core structure. The results also show that the HR2 proteins of NiV and HeV are functionally and structurally...

Ngày tải lên: 08/03/2014, 08:20

... NMR structure of SUMO-1 and the crystal structure of yeast A CD B Fig. 2. Photographs and electron density maps of the SUMO-2 crystals. Shown in (A) and (B) are two different crystal forms I and II obtaine ... type II crystal, r efinements and map calculations also used SHELX -97 [14]. Substitution of side chains, addition of water molecules, manual adjustment of th...

Ngày tải lên: 16/03/2014, 18:20

... con- tained a 1 : 1 ratio, by volume, of reservoir solution and protein. The final drop was typically 2 lL, and crystals grew in 2–7 days. The wild-type and PR V82A crystals were obtained using 0.05 M citrate/phosphate ... at the termini and the variable surface loops of residues 16–18, 37–41, and 67– 69 of both subunits in all structures, except for the larger values at 50–5...

Ngày tải lên: 23/03/2014, 12:20

... UND Phe89 A Alt Open Open Alt Alt Open B Alt Alt Alt Closed Alt Closed Volumes of the cavities A 396 491 504 396 400 443 B 377 378 401 375 377 377 Ó FEBS 2004 Crystal structures of bOBP–odorant ... 1). Results Binding of AMA and odorants in solution The dissociation c onstants of the AMA–OBP c omplexes fo r the bovine and porcine OBP isoforms were, respectively, 1 .0...

Ngày tải lên: 30/03/2014, 15:20

... the PLP and PMP forms, 87% of the residues were in the most favored regions of the Ramachandran plot. Although Tyr286 (A) and (B) in both forms fall within a disallowed region of the Ramachandran ... Asp112 and Lys6 (3.96, 4.15 A ˚ ) and Asp112 and Arg7 (3.48, 3.55 A ˚ ) of the opposite subunits. In AeKAT structures, Cys284 (A) and Cys284 (B) with distances of 3.46...

Ngày tải lên: 30/03/2014, 16:20