Báo cáo khoa học: Thiamin diphosphate in biological chemistry: analogues of thiamin diphosphate in studies of enzymes and riboswitches pot
Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf
... pantotrophus. In uence of variations of conserved residues on the in vivo activity of NirF Interestingly, like NirN, NirF shares sequence similar- ity with the C-terminal d 1 heme-containing domain of cytochrome ... and of the kanamycin insertion mutant nirF strain (SBN03) in the upper right panel. Growth of SBN11 (DnirF) is shown in the bottom left panel and of SBN13...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
... targets). Effects of DNA cis-platination on sequence- specific DNA binding of p73 proteins We tested the in uence of DNA modification with cisplatin on the binding of two p73 isoforms, p73d and p73b, to the p21 ... weak p53 binding (about 4% for mdm2 and PGM1 and 10% for p21). Sensitivity of the sequence-specific p53 DNA binding to DNA cis-platination depends on the incidence o...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf
... represents the amino terminus of the pro- tein. Using this method, we showed that asparagine 26 represents the amino terminus of P40. Knowing, that the first amino acids of the processed P40 and P90 is the ... state of this peptide is unexpected: a peptide containing two basic amino acids (arginine and histidine) and a blocked amino terminus should not acquire more than two posit...
Ngày tải lên: 19/02/2014, 18:20
Tài liệu Báo cáo khoa học: Trophoblast-like human choriocarcinoma cells serve as a suitable in vitro model for selective cholesteryl ester uptake from high density lipoproteins pdf
... C-terminal cytosolic domain of SR-BI which is a critical domain involved in selective CE-uptake [29,53] is lacking in SR-BII and the lack of SR-BII protein in all three choriocarcinoma cell lines investigated ... choriocarcinoma cells (24 h in DMEM containing 10% FBS, 1% glutamine and 1% penicillin/streptomycin) and CHO cells (HAM’s F12 medium containing 5% (v/v) FBS, 2 m M...
Ngày tải lên: 20/02/2014, 23:20
Báo cáo khoa học: Visfatin is induced by peroxisome proliferator-activated receptor gamma in human macrophages pdf
... data AcLDL 1 2 Visfatin/cyclophilin mRNAVisfatin/cyclophilin mRNA Visfatin/cyclophilin mRNA CD36/cyclophilin mRNA FABP4/cyclophilin mRNA Visfatin/cyclophilin mRNA Visfatin/cyclophilin mRNA Visfatin/cyclophilin mRNA 3 4 3 ... adipocytes, and mostly by macrophages, and circulates in the plasma of humans and mice [4]. Plasma concentrations of visfa- tin are positively associated with...
Ngày tải lên: 06/03/2014, 22:21
Báo cáo khoa học: A novel prokaryotic L-arginine:glycine amidinotransferase is involved in cylindrospermopsin biosynthesis potx
... confirm incorporation of ubiquitously labeled arginine into cyl- indrospermopsin [23]. However, the transamidination of glycine from arginine by amidinotransferase, yield- ing guanidinoacetate, is common in vertebrates, ... performances. However, the kinetic mechanism of CyrA in the pres- ence of l-arginine and glycine as substrates differs from the well-established ping-pong mec...
Ngày tải lên: 06/03/2014, 22:21
Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt
... predicting the role of Ca 2+ and Ca 2+ -binding proteins in biological systems. Materials and methods Molecular cloning and modelling of engineered CD2.STIM1.EF The single EF-hand motif in STIM1 ... distance between the two termini of the inserted Ca 2+ -binding sites in the model structure of the EF-hand of STIM1 is within 15 A ˚ . Accordingly, a total of six glycin...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Ionizing radiation utilizes c-Jun N-terminal kinase for amplification of mitochondrial apoptotic cell death in human cervical cancer cells pptx
... carcinoma cells [13]. Further studies have shown that phosphorylation of Bcl-2 on residues of in its loop domain, including Ser70 and Ser87, is critically involved in the apoptotic process, and ... were introduced into the cells using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s recommendations. Quantification of cell death Apoptosis was investigated by Annex...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: The calpain 1–a-actinin interaction Resting complex between the calcium-dependant protease and its target in cytoskeleton doc
... Calp1 binding site is included in the C-terminal domain of the ask molecule, and this binding is independent of the susceptibility of the a-actinin isoform to calpain proteolysis. Identification of ... (domains III–IV) of the 80 kDa subunit, are implicated in the interface linking calpain 1 to skeletal muscle a-actinin. Colocalization of microcalpain and a-actinin in myog...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: 15-Deoxy D12,14-prostaglandin J2 suppresses transcription by promoter 3 of the human thromboxane A2 receptor gene through peroxisome proliferator-activated receptor c in human erythroleukemia cells ppt
... in TPb mRNA expression in response to 15d-PGJ 2 in HEL cells was reflected in a corresponding reduction in the overall level of TP expression, as assessed by radioligand binding studies, and in ... vasculature and point to further critical differences into the modes of regulation of TPa and TPb in humans. Moreover, in view of the critical link between the enhance...
Ngày tải lên: 07/03/2014, 21:20