Báo cáo khoa học: Thiamin diphosphate in biological chemistry: analogues of thiamin diphosphate in studies of enzymes and riboswitches pot

Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf

... pantotrophus. In uence of variations of conserved residues onthe in vivo activity of NirFInterestingly, like NirN, NirF shares sequence similar-ity with the C-terminal d1heme-containing domain of cytochrome ... and of thekanamycin insertion mutant nirF strain(SBN03) in the upper right panel. Growth of SBN11 (DnirF) is shown in the bottom leftpanel and of SBN13 (DnirF) containingpEG276-nirF in the ... courses of nitrite accumulation and consumption in Paracoccus pantotro-phus strains. Starter cultures were grownaerobically in LB with shaking before inocu-lation of mineral salt medium containing20...

Tài liệu Báo cáo khoa học: DNA modiﬁcation with cisplatin affects sequence-speciﬁc DNA binding of p53 and p73 proteins in a target site-dependent manner pptx

... targets).Effects of DNA cis-platination on sequence-speciﬁc DNA binding of p73 proteinsWe tested the in uence of DNA modiﬁcation withcisplatin on the binding of two p73 isoforms, p73d and p73b, to the p21 ... weakp53 binding (about 4% for mdm2 and PGM1 and 10% for p21).Sensitivity of the sequence-speciﬁc p53 DNAbinding to DNA cis-platination depends on theincidence of cisplatin-reactive motifs within ... obtained from densitometric tracing of the autoradiograms; for each targetsite, the intensity of the R53band resulting from p53 binding to unmodiﬁed DNA was taken as 1.0, and the intensities of...

Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf

... represents the amino terminus of the pro-tein. Using this method, we showed that asparagine 26represents the amino terminus of P40. Knowing, thatthe ﬁrst amino acids of the processed P40 and P90 isthe ... state of thispeptide is unexpected: a peptide containing two basicamino acids (arginine and histidine) and a blockedamino terminus should not acquire more than twopositive charges in positive ... labeled within the intactprotein at its a-amino group and represents the aminoterminal amino acid of P40. The efﬁciency of thisreaction was quite high, as judged from the signalintensities of the...

Tài liệu Báo cáo khoa học: Trophoblast-like human choriocarcinoma cells serve as a suitable in vitro model for selective cholesteryl ester uptake from high density lipoproteins pdf

... C-terminal cytosolic domain of SR-BI which is a critical domain involved in selectiveCE-uptake [29,53] is lacking in SR-BII and the lack of SR-BII protein in all three choriocarcinoma cell linesinvestigated ... choriocarcinomacells (24 h in DMEM containing 10% FBS, 1% glutamine and 1% penicillin/streptomycin) and CHO cells (HAM’sF12 medium containing 5% (v/v) FBS, 2 mMgluta-mine, 50 UÆmL)1penicillin and ... [31]. Speciﬁc binding (4 °C) was calculated as thedifference between total and nonspeciﬁc binding [37].To determine cell-associated (the sum of binding and internalization) and degraded125I-labeled...

Báo cáo khoa học: Visfatin is induced by peroxisome proliferator-activated receptor gamma in human macrophages pdf

... dataAcLDL12Visfatin/cyclophilin mRNAVisfatin/cyclophilin mRNAVisfatin/cyclophilin mRNACD36/cyclophilin mRNAFABP4/cyclophilin mRNAVisfatin/cyclophilin mRNAVisfatin/cyclophilin mRNAVisfatin/cyclophilin mRNA343 ... adipocytes, and mostlyby macrophages, and circulates in the plasma of humans and mice [4]. Plasma concentrations of visfa-tin are positively associated with cytokines such asinterleukin (IL)-6 and increase ... EDTA, 10% glycerol, 1 mgÆmL)1 of BSA) contain-ing 1 lg of poly(dI-dC) and 1 lg of herring sperm DNA in a total volume of 20 lL. Double-stranded oligonucleotidescontaining the wild-type DR1–PPRE,...

Báo cáo khoa học: A novel prokaryotic L-arginine:glycine amidinotransferase is involved in cylindrospermopsin biosynthesis potx

... conﬁrmincorporation of ubiquitously labeled arginine into cyl-indrospermopsin [23]. However, the transamidination of glycine from arginine by amidinotransferase, yield-ing guanidinoacetate, is common in vertebrates, ... performances.However, the kinetic mechanism of CyrA in the pres-ence of l-arginine and glycine as substrates differsfrom the well-established ping-pong mechanism of l-arginine:glycine amidinotransferase, ... Biosynthesis of homoarginine and ornithine as precursors of thephytoeffector phaseolotoxin by the amidinotransferfrom arginine to lysine catalysed by an amidinotransfer-ase in Pseudomonas syringae...

Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt

... predicting the role of Ca2+ and Ca2+-binding proteins in biological systems.Materials and methodsMolecular cloning and modelling of engineeredCD2.STIM1.EFThe single EF-hand motif in STIM1 ... distance betweenthe two termini of the inserted Ca2+-binding sites in the model structure of the EF-hand of STIM1 is within15 A˚. Accordingly, a total of six glycine linkers is sufﬁ-cient to ... dimerization and calcium binding in an EF-hand synthetic peptide. J Theor Biol 199, 199–211.12 Julenius K, Robblee J, Thulin E, Finn BE, Fairman R& Linse S (2002) Coupling of ligand binding and...

Báo cáo khoa học: Ionizing radiation utilizes c-Jun N-terminal kinase for ampliﬁcation of mitochondrial apoptotic cell death in human cervical cancer cells pptx

... carcinoma cells [13]. Further studies haveshown that phosphorylation of Bcl-2 on residues of in its loop domain, including Ser70 and Ser87, is criticallyinvolved in the apoptotic process, and ... were introduced intothe cells using Lipofectamine 2000 (Invitrogen) accordingto the manufacturer’s recommendations.Quantiﬁcation of cell deathApoptosis was investigated by Annexin V labeling ... next examinedwhether caspase-8 is involved in radiation-inducedactivity-related modulations of the conformation of Bax and Bak. Inhibition of caspase-8 by a speciﬁcinhibitor, caspase 8 inhibitor...

Báo cáo khoa học: The calpain 1–a-actinin interaction Resting complex between the calcium-dependant protease and its target in cytoskeleton doc

... Calp1 binding site isincluded in the C-terminal domain of the ask molecule, and this binding is independent of the susceptibility of thea-actinin isoform to calpain proteolysis.Identiﬁcation of ... (domains III–IV) of the 80 kDa subunit,are implicated in the interface linking calpain 1 to skeletalmuscle a-actinin.Colocalization of microcalpain and a-actinin in myogenic cellsCalpains and ... or in acomplex including the two proteins. In conclusion, our study proves the interaction betweena-actinin and calpain 1 and locates binding motifs withinregions where the EF-hand domains of...

Báo cáo khoa học: 15-Deoxy D12,14-prostaglandin J2 suppresses transcription by promoter 3 of the human thromboxane A2 receptor gene through peroxisome proliferator-activated receptor c in human erythroleukemia cells ppt

... in TPb mRNA expression in response to 15d-PGJ2 in HEL cells was reﬂected in acorresponding reduction in the overall level of TPexpression, as assessed by radioligand binding studies, and in ... vasculature and point to further critical differencesinto the modes of regulation of TPa and TPb in humans. Moreover, in view of the critical link betweenthe enhanced risk of cardiovascular disease in ... signiﬁcantlevels of functional PPARc [48] and thereby impliesthat PPARc isoforms may have a role in mediatingthe 15d-PGJ2–inhibition of Prm3 activity. Theinvolvement of both PPARc and the retinoic...

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