Báo cáo khoa học: Ca2+ ⁄ H+ antiporter-like activity of human recombinant Bax inhibitor-1 reconstituted into liposomes pdf

... Ca 2+ ⁄ H + antiporter-like activity of human recombinant Bax inhibitor-1 reconstituted into liposomes Taeho Ahn 1 , Chul-Ho Yun 2 , Ho Zoon Chae 2 , ... February 2009) doi:10.1111/j.1742-4658.2009.06956.x We investigated the functional activity of recombinant Bax inhibitor-1 reconstituted into liposomes. When proteoliposomes were suspended in acidic sol...

Ngày tải lên: 29/03/2014, 23:20

... l M of OsDmc1 in absence of ATP; (2) with 1.25 lM of OsDmc1 in presence of 2.0 m M of AMP-PNP; (3) with 1.25 lM of OsDmc1 in presence of 2.0 mM ATP-c-S; (4) with 1.25 lM of OsDmc1 in presence of ... stimulated a significant level of DNA dependent ATPase activity. OsDmc1 also showed renaturation of complementary strands as well as assimilation of single strands into h...

Ngày tải lên: 19/02/2014, 07:20

... herein are those of the authors and do not reflect the of cial position of the US Army or the Department of Defense. Guidelines for human experimentation of the US Depart- ment of Defense were ... were developed to predict the order of potency of eight adenosine analogs for the inhibition of human AdoHcy hydrolase. In view of the potent antiviral activities of the DZA...

Ngày tải lên: 08/03/2014, 08:20

... the routing of the receptor to the cell surface, resulting in loss of basal activity and loss of agonist induced cAMP production [14–16]. In order to study the mechanism of activation of the TSHr ... the N-terminal domain of the receptor translates into activation of its serpentine domain is unknown. With the aim of improving our understanding of the mechanisms underlyin...

Ngày tải lên: 08/03/2014, 08:20

... (1977) Characteristics of a human cell line transformed by DNA from human adenovirus type. J Gen Virol 36, 59–74. 40 Graham FL & van der Eb AJ (1973) A new technique for the assay of infectivity of human ... DpnI into a fragment of 2162 bp (Figs 1B and 3A,B, marked with filled squares). On day 3 post transfection, a full- length TTV DNA of 3748 bp (after BamHI digestion,...

Ngày tải lên: 16/03/2014, 05:20

... PC1 ⁄ PC3, PC4, PACE4, PC5 ⁄ PC6 and LPC ⁄ PC7 ⁄ PC8 ⁄ SPC7) have been identified in mammalian species [2]. Furin was the first identified mammalian PC and the most extensively studied member of ... gradient of 0–20% acetonitrile in 0.1% (v ⁄ v) trifluoroacetic acid at a flow rate of 1 mLÆmin )1 in 0–20 min, and of 20–100% acetonitrile in 0.1% (v ⁄ v) trifluoroac...

Ngày tải lên: 16/03/2014, 13:20

... Mg 2+ transport activity of Lpe10p The conserved F ⁄ YGMN motif in TM1 of CorA-like proteins cannot be varied without loss of Mg 2+ uptake [4,18]. We performed site-directed mutagenesis, replacing the F ⁄ ... Mg 2+ - dependent influx. High copy number expression of Lpe10p led to an increased rate of uptake of Mg 2+ upon addition of 1 and 3 mm [Mg 2+ ] e . Expression of...

Ngày tải lên: 29/03/2014, 21:20

... Chemiluminescence of the band linearly correlated with the amount of recombinant S100A9 (25 to 200 ng per lane). For quantitation of S100A9 in the exudates, three different amounts of recombinant S100A9 (80, ... presence (+) of 2-mercaptoethanol (final 10%) and stained with Coomassie brilliant blue. Ó FEBS 2004 Fibroblast growth-stimulating activity of S100A9 (Eur. J. Biochem...

Ngày tải lên: 30/03/2014, 13:20

... mAb TC2 plus pairs 3-9 ⁄ 5H2, 3-9 ⁄ 3C12, 4-7 ⁄ TC7, 2-2 ⁄ 3C12, TC4 ⁄ 5H2, TC4 ⁄ TC7, TC4 ⁄ 3C12; and (b) capturing mAb 3-9 plus pairs 4-7 ⁄ 3C4, 2-2 ⁄ 3C4. Synthesis of biotinylated Cbl Cbl ... 3-9 was used for pairs 4-7 ⁄ 3C4 and 2-2 ⁄ 3C4, where binding of the second mAb of the pair was inhibited by < 20%. Interaction of mAbs with the...

Ngày tải lên: 20/02/2014, 01:20

... composed of features like POS, the number of complements, category of each complement, and the position of comple- ments. In their view, structural disambiguation is simply another type of lexical ... pair of sentences. Comparatively faster or larger drop of probability is expected to be a good indicator of comparative processing difficulty. Probability re- ranking, which is a...

Ngày tải lên: 20/02/2014, 11:21