Báo cáo khoa học: Epitope analysis of the rat dipeptidyl peptidase IV monoclonal antibody 6A3 that blocks pericellular fibronectin-mediated cancer cell adhesion pot
... Epitope of monoclonal DPP IV antibody FEBS Journal 276 (2009) 6548–6559 ª 2009 The Authors Journal compilation ª 2009 FEBS 6559 Epitope analysis of the rat dipeptidyl peptidase IV monoclonal antibody ... adhesion between the lung endothelial adhesion receptor dipeptidyl peptidase IV (DPP IV) and pericellular polymeric Keywords dipeptidyl pepti...
Ngày tải lên: 29/03/2014, 22:21
... series of succes- sive events that lead to the progressive reduction of the quinone molecules located on the acceptor side of PSII [3]. The progressive reduction of the acceptor side of PSII ... absence of additives or in the presence of 1 lm DCMU or dPQ over a range of temperature from 15 °Cto25°C. The range of tem- perature was set on the basis of the...
Ngày tải lên: 19/02/2014, 05:20
... levels of expression. The transcriptional activity of FLAG-BMAL1, and the suppressive activity of FLAG- DEC1, were s imilar to those of intact BMAL1 and DEC1, respectively (Fig. 1A,C). These findings ... Interaction of DEC1 with BMAL1 (Eur. J. Biochem. 271) 4413 The basic region of DEC1 is essential for its suppressive activity To further n arrow down the region required fo...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Fluorescence analysis of the Hansenula polymorpha peroxisomal targeting signal-1 receptor, Pex5p pdf
... Laboratory of Biochemistry, Wageningen University, the Netherlands; 4 Centre for Molecular and Biomolecular Informatics, University of Nijmegen, the Netherlands; 5 Department of Biology, University ... at the emission maximum) with that of the standard N-Ac-Trp- NH 2 normalized to the same absorbance at 295 nm. A value of 0.13 was used for the quantum yield of the...
Ngày tải lên: 21/02/2014, 00:20
Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf
... linear regression analysis of the logarithms of the concentration ratios for oxidized and reduced forms of the mediator versus that of recombinant flavoredoxin. Reduction titration of flavoredoxin by NAD(P)H The ... 3D). Redox potential of recombinant flavoredoxin Figure 4 shows the results of linear regression analysis of the logarithms for the redox ratio o...
Ngày tải lên: 07/03/2014, 02:20
Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt
... of complex stability, but rather at the level of activity or substrate specificity. The apparent discrepancies between studies in yeast and mammalian cells indicate that the TOR signaling pathway ... show that TIPRL directly binds the catalytic subunits of type 2A phosphatases, but not a4, and that it regulates the activity of PP2A. These findings contrast with the mod...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Epitope mapping of the O-chain polysaccharide of Legionella pneumophila serogroup 1 lipopolysaccharide by saturation-transfer-difference NMR spectroscopy pot
... a-andb-con®guration in a ratio of approximately 5 : 1, which is also the case for free Rha II in the other 30% of the molecules. The anomeric con®guration of the ketosidic linkage of the legionaminic ... to map the mAb 2625 epitope on a molecular level. It could be demonstrated that the b inding anity of the N-methylated legionaminic acid derivatives was indep...
Ngày tải lên: 08/03/2014, 10:20
Báo cáo khoa học: Expression analysis of the nucleocytoplasmic lectin ‘Orysata’ from rice in Pichia pastoris ppt
... (syncytium) cell for- mation was recorded microscopically in the CEM cell cul- tures, and the number of giant cells was estimated as the percentage of the number of giant cells present in the non- treated ... clumping of the cells at the bottom of the glass tube. Samples that yielded no visible agglutination activity after incubation for 1 h were regarded as le...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot
... retaining in that the anomeric configuration of the glucose is the same in the product (b-d-glucose) as it is in the substrate (a b-d-glucosides). Substrate hydrolysis requires the par- ticipation of two ... introducing features of the consensus motif into the Zm-p60.1 scaffold, an analysis of structural and catalytic prop- erties of the mutants, and simulations...
Ngày tải lên: 16/03/2014, 04:20
Báo cáo khoa học: Mutational analysis of the C-domain in nonribosomal peptide synthesis pot
... the timing of substrate epimerization [7]. Recognition and activation of the substrate amino acid are facilitated by the A-domain [8] through carboxy adenylation of the substrate. Hence, the substrate ... substrate selectivity of the A-domains [9] simultaneously determines the primary sequence of the product. Subsequently the a ctivated amino acid i s tethered to...
Ngày tải lên: 17/03/2014, 11:20