Báo cáo khoa học: Relaxin-3⁄ insulin-like peptide 7, a neuropeptide involved in the stress response and food intake Masaki Tanaka pptx

... MINIREVIEWRelaxin-3⁄ insulin-like peptide 7, a neuropeptide involved in the stress response and food intake Masaki Tanaka Department of Basic Geriatrics, Kyoto Prefectural University ... Relaxin-3 is so named because it has a motif thatcan interact with the relaxin receptor. By contrast to other relaxins,relaxin-3 is mainly expressed in the brain and testis. In rodent brain, ana-tomical ... 1A) , a smaller number of these neurons arescattered in the pontine raphe nucleus, the periaqu-eductal gray matter, and the area dorsal to the substantia nigra in the midbrain reticular formation.By...

Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx

... N-terminal hairpin in HtA isintermediate between those in RNse T1 and a- sarcin,having 20 amino acids in HtA, 26 in a- sarcin and 12 in RNase T1. From a functional point of view, thisreduction in ... in HtA replaces a tyrosine of a- sarcin, and the aromaticring of F126, close to the catalytic H113, replaces a leucine side chain in a- sarcin in a similar arrangementto that found in RNase T1. ... [16].E96 and H137 in a- sarcin and E58 and H92 in RNaseT1 act as the base and acid groups, respectively. Com-paring the 3D structures, E66 and H113 in HtA are in similar positions to those pairs and...

Báo cáo khoa học: Transmembrane helix 12 plays a pivotal role in coupling energy provision and drug binding in ABCB1 pot

... the intracellular and extracellular sections of the helix in the basal and ATP-bound states of the model, and thatthis section of TM12 appeared to act as an anchoraround which the rest of the ... and, in particular, in coordinating conformational changes between the NBDs and transmembrane domains.AbbreviationsABC, ATP-binding cassette; AMP-PNP, 5¢-adenylylimidodiphosphate; BM, BODIPY maleimide; ... label-ling according to the following relationship:t1=2¼Ln2=kStatistical analysisAll data manipulations and statistical analyses were per-formed using graphpad prism 4.0. Comparison of datasetsfor...

Báo cáo khoa học: Identiﬁcation of two cysteine residues involved in the binding of UDP-GalNAc to UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) ppt

... putative catalytic domaincontains two distinct subdomains; a central catalyticdomain and a C-terminal lectin-like domain. The centralcatalytic domain can be further subdivided into two regions. The ... sequence.Highlyconservedcysteineresiduesarerepresentedbydottedlines.b,r,hT1,bovine,rat,andhumanGalNAc-T1;hT2,humanGalNAc-T2;hT3,human GalNAc-T3; hT4, human GalNAc-T4; rT5, rat GalNAc-T5; hT6, human GalNAc-T6; hT7, human GalNAc-T7; hT8, human GalNAc-T8;hT9, human GalNAc-T9; ... C10 6a, 5¢-TAGAGGGGGCTAAAACAAAA-3¢; c21 2a, 5¢-ACTGTGCACTCGGCGTGAGC-3¢; c21 4a, 5¢-ACTGTGGCCTCGCAGTGAGC-3¢;c23 5a, 5¢-TAGGAGCCACCACTGTCCTC-3¢; c33 0a, 5¢-CAGAGTCCCTCCAGCCTGCC-3¢; c33 9a, 5¢-GGAGGCCGTCACTATTTCCA-3¢;...

Báo cáo khoa học: An E3 ubiquitin ligase, Synoviolin, is involved in the degradation of immature nicastrin, and regulates the production of amyloid b-protein doc

... N, Kawarai T, Hasegawa H,Duthie M, Li W, Ruan X, Luthra A, Mount HT et al.(2003) APH-1 interacts with mature and immatureforms of presenilins and nicastrin and may play a role in maturation ... is involved in the degrada-tion of endogenous immature nicastrin, and affects amyloid b-protein genera-tion. It was found that the level of immature nicastrin was dramaticallyincreased in ... (Berkeley, CA,USA). Anti-PEN-2 IgM was provided by Dr Thinakaran[ 27,2 8]. Anti -a- tubulin and anti-calnexin IgG were pur-chased from Santa Cruz Biotechnology, Inc. (Santa Cruz,CA, USA). Anti-APP N-terminal...

Báo cáo khoa học: dehydrogenase from Arabidopsis thaliana, a ﬂavoprotein involved in vitamin C biosynthesis pot

... ofother aldonolactone oxidoreductases in the biosynthesis of vitamin C in A. thaliana are also discussed.AbbreviationsALO,D-arabinono-1,4-lactone oxidase; AtGALDH, Arabidopsis thaliana L-galactono-1,4-lactone ... PCR ampliﬁed from A. thaliana(ecotype Columbia) seedling cDNA, using the oligo-nucleotides AtGALDH_fw102 (5¢-GGAATTCCATATGTACGCTCCTTTACCTGAAG-3¢) and AtGALDH_rv(5¢-CCGCTCGAGAGCAGTGGTGGAGACTG-3¢), ... FAD-binding domain (Fig. 2). From the alignment, it is clear that GALDH in plants lacks the histidine residue involved in covalent ﬂavinylation in GUO, ALO and GLO, but contains a leucine resi-due instead...

Báo cáo khoa học: Light regulation of CaS, a novel phosphoprotein in the thylakoid membrane of Arabidopsis thaliana doc

... 5¢-GTGTAGCGCGCGTGCGGCCC-3¢. The forward and reverse primers, respectively,for CaS were 5¢-AAATGGCAACGAAGTCTTCAC-3¢ and 5¢-CAGTCGGAGCTAGGAAGGAA-3¢.Isolation of plasma membrane, intactchloroplasts, stroma and thylakoids The ... to be involved in signal transduction and stress responses and also in protein import intochloroplasts [32]. FHA domain proteins are directly involved in signal transduction, and the interactionbetween ... (N-terminal) and y-ions (C-terminal) are indicated in the spectrum as well as in the corresponding amino acid sequence. The ions marked with an asterisk indicate that the fragments underwent neutral...

Báo cáo khoa học: Acylation of lysophosphatidylcholine plays a key role in the response of monocytes to lipopolysaccharide ppt

... bovineserum albumin (BSA; 1%, w/v) and sucrose (5%, w/v) in NaCl/Piwere added to each well and the plates incubatedfor at least 1 h and washed as above. Human recombinantTNF -a (R&D) was ... added and the plate incubated for 20 min. The plate was washed and 100 lL of tetramethylbenzidine substrate containing0.01% H2O2(Kirkegaard and Perry, Gaithersburgh, MD,USA) per well. The plate ... plate was incubated in the dark for20 min and the reaction stopped by the addition of 50 lLof0.5Msulfuric acid. The plate was read on a LabsystemsMultiskan plate reader using the absorbance...

Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

... biotin and the target proteins, and thus allows their analysis by SDS ⁄ PAGE.Subcellular fractionation of porcine striatal braintissuePorcine brain cut in half in the saggital plane was obtainedfresh ... membrane staining of DAT and morediffuse cytoplasmic p2 5a staining. To better visualize the possibleco-localization of p2 5a and DAT, the neurites indicated by the inset in the lower left panel are ... together with granular intracellular stainingcompatible with an association with vesicles and the plasma membrane. p2 5a showed cytosolic staining, waslocalized in the cytosol with a less granular...

Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc

... analysis with the antibody against ETA revealed that the endosomalETA-degrading activity was partially inhibited by the aspartic acid protease inhibitor pepstatin -A (PA), the cysteine protease ... efﬁciently increased by a cathepsin B ⁄ cathepsin Dpretreatment. The data show a novel processing pathway for internalizedETA, involving cathepsins B and D, resulting in the production ofETA fragments ... generated in vitro occurring mainly within domain III ofETA -A. Cell-free endosomes preloaded in vivo with ETA intraluminallyprocessed and extraluminally released intact ETA and ETA -A in vitro in a pH-dependent...

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