Báo cáo khoa học: Relaxin-3⁄ insulin-like peptide 7, a neuropeptide involved in the stress response and food intake Masaki Tanaka pptx

... MINIREVIEW Relaxin-3 ⁄ insulin-like peptide 7, a neuropeptide involved in the stress response and food intake Masaki Tanaka Department of Basic Geriatrics, Kyoto Prefectural University ... Relaxin-3 is so named because it has a motif that can interact with the relaxin receptor. By contrast to other relaxins, relaxin-3 is mainly expressed in the brain and...

Ngày tải lên: 29/03/2014, 21:20

... N-terminal hairpin in HtA is intermediate between those in RNse T1 and a- sarcin, having 20 amino acids in HtA, 26 in a- sarcin and 12 in RNase T1. From a functional point of view, this reduction in ... in HtA replaces a tyrosine of a- sarcin, and the aromatic ring of F126, close to the catalytic H113, replaces a leucine side chain in a- sarcin in a sim...

Ngày tải lên: 18/02/2014, 08:20

... the intracellular and extracellular sections of the helix in the basal and ATP-bound states of the model, and that this section of TM12 appeared to act as an anchor around which the rest of the ... and, in particular, in coordinating conformational changes between the NBDs and transmembrane domains. Abbreviations ABC, ATP-binding cassette; AMP-PNP, 5¢-adenylylimid...

Ngày tải lên: 06/03/2014, 22:21

... putative catalytic domain contains two distinct subdomains; a central catalytic domain and a C-terminal lectin-like domain. The central catalytic domain can be further subdivided into two regions. The ... sequence. Highlyconservedcysteineresiduesarerepresentedbydottedlines.b,r,hT1,bovine,rat,andhumanGalNAc-T1;hT2,humanGalNAc-T2;hT3, human GalNAc-T3; hT4, human GalNAc-T4; rT5, rat Ga...

Ngày tải lên: 08/03/2014, 10:20

... N, Kawarai T, Hasegawa H, Duthie M, Li W, Ruan X, Luthra A, Mount HT et al. (2003) APH-1 interacts with mature and immature forms of presenilins and nicastrin and may play a role in maturation ... is involved in the degrada- tion of endogenous immature nicastrin, and affects amyloid b-protein genera- tion. It was found that the level of immature nicastrin was dramatically...

Ngày tải lên: 23/03/2014, 04:20

... of other aldonolactone oxidoreductases in the biosynthesis of vitamin C in A. thaliana are also discussed. Abbreviations ALO, D-arabinono-1,4-lactone oxidase; AtGALDH, Arabidopsis thaliana L-galactono-1,4-lactone ... PCR amplified from A. thaliana (ecotype Columbia) seedling cDNA, using the oligo- nucleotides AtGALDH_fw102 (5¢-GGAATTC CATATG TACGCTCCTTTACCTGAAG-3¢) and AtGALDH_rv (...

Ngày tải lên: 23/03/2014, 07:20

... 5¢-GTGTAGCGCGCGTGCG GCCC-3¢. The forward and reverse primers, respectively, for CaS were 5¢-AAATGGCAACGAAGTCTTCAC-3¢ and 5¢-CAGTCGGAGCTAGGAAGGAA-3¢. Isolation of plasma membrane, intact chloroplasts, stroma and thylakoids The ... to be involved in signal transduction and stress responses and also in protein import into chloroplasts [32]. FHA domain proteins are directly...

Ngày tải lên: 30/03/2014, 04:20

... bovine serum albumin (BSA; 1%, w/v) and sucrose (5%, w/v) in NaCl/P i were added to each well and the plates incubated for at least 1 h and washed as above. Human recombinant TNF -a (R&D) was ... added and the plate incubated for 20 min. The plate was washed and 100 lL of tetramethylbenzidine substrate containing 0.01% H 2 O 2 (Kirkegaard and Perry, Gaithersburgh, MD,...

Ngày tải lên: 31/03/2014, 01:20

... biotin and the target proteins, and thus allows their analysis by SDS ⁄ PAGE. Subcellular fractionation of porcine striatal brain tissue Porcine brain cut in half in the saggital plane was obtained fresh ... membrane staining of DAT and more diffuse cytoplasmic p2 5a staining. To better visualize the possible co-localization of p2 5a and DAT, the neurites indicated by t...

Ngày tải lên: 14/02/2014, 21:20

... analysis with the antibody against ETA revealed that the endosomal ETA-degrading activity was partially inhibited by the aspartic acid protease inhibitor pepstatin -A (PA), the cysteine protease ... efficiently increased by a cathepsin B ⁄ cathepsin D pretreatment. The data show a novel processing pathway for internalized ETA, involving cathepsins B and D, resulting in the...

Ngày tải lên: 18/02/2014, 04:20