Báo cáo khoa học: Structure and function of human a-lactalbumin made lethal to tumor cells (HAMLET)-type complexes pptx

... 4625 MINIREVIEW Structure and function of human a -lactalbumin made lethal to tumor cells (HAMLET)-type complexes Ann-Kristin Mossberg 1 , Kenneth Hun Mok 1,2 , Ludmilla A. Morozova-Roche 3 and Catharina ... 2010) doi:10.1111/j.1742-4658.2010.07890.x Human a-lactalbumin made lethal to tumor cells (HAMLET) and equine lysozyme with oleic acid (ELOA) a...

Ngày tải lên: 29/03/2014, 21:20

... with binding of both MOF and Tip60 (equivalent to Esa1 in yeast NuA4) [124,130]. However, in human cells depletion of MOF, but not Tip60, results in reduced global levels of AcH4-K16 and defective ... histone acetylation and deacetyla- tion, and transient recruitment of remodellers and transcription factors. A cycle of transcription commences with the recruit- ment...

Ngày tải lên: 14/02/2014, 18:20

... form of cyprosin was processed in different isoforms by the excision of the prosegment and of most of the PSI [21]. Conversely to what has been found in vivo [31], heavy and light chains of the ... Biochemical and immunocytochemistry analysis of barley roots and leaves showed that phytepsin was localized to the vacuoles of these cells [80] and, in a different stud...

Ngày tải lên: 19/02/2014, 12:20

... which the b1-strand and b2-strand are adjacent and parallel to each other, and the b¢-strand is adja- cent and antiparallel to the b1-strand (Fig. 1). The length and sequence of the variable ... a1-helix and a2- helix and the b2-strand of KH1. Intimate contacts between protein and RNA continue across the cusp of the KH1 and KH2 domains. C46 binds to the Fig. 6. C...

Ngày tải lên: 07/03/2014, 05:20

... characterization of GlcNAc kinase and cysteine mutants In order to get a more detailed insight into the functions of specific cysteine residues of GlcNAc kinase, the K m values for both substrates, GlcNAc and ... chloramphenicol and ampi- cillin. Cells were grown to an absorbance of 0.5, induced with 1m M isopropylthio-a-galactoside for 2 h, harvested and resuspended in 2...

Ngày tải lên: 08/03/2014, 10:20

... (rmsd of 1.6 A ˚ for 1192 Ca atoms of a tetramer and 1.44 for 306 Ca atoms of a monomer). This indicates that not only is the monomer structure conserved, but also the quaternary structure of the tetramer. ... Rev Microbiol 50, 553–590. 3 Ornston LN (1966) The conversion of catechol and protocatechuate to b-ketoadipate by Pseudomonas put- ida. II. Enzymes of the protocat...

Ngày tải lên: 30/03/2014, 10:20

... Sciences, Turku, Finland). Estimation of expression of cytosolic regulatory proteins Total cell lysates of transfected CHO cells were used to esti- mate expression of Noxo1b, Noxo1c, and Noxo1a. The lysates ... the following human cell lines; androgen-independent prostate cancer LNCaP cells, androgen-independent prostate cancer PC3 and DU145 cells, and testicular germ c...

Ngày tải lên: 19/02/2014, 06:20

... expres- sion of the raptor or rictor genes (partners of mTORC1 and mTORC2 respectively) has only a slight effect on the translation efficiency of TOP mRNAs. This result implies that mTOR regulates TOP ... sclerosis complex (TSC)1– TSC2, and the mammalian target of rapamycin (mTOR) C1 complex (composed of raptor, mLst8, and mTOR). The role of TSC1–TSC2 in the translation of...

Ngày tải lên: 07/03/2014, 01:20

... a-helices. Residues involved in binding of the cofactor ThDP are located at the C-terminal ends of the b-strands of Dom-c (diphosphates and Mg 2+ ) and of Dom-a¢ of a neighbouring subunit (pyrimidine moiety). ... Superposition of the ThDP cofactors of BAL (grey), ALS (cyan), AHAS (green), IPDC (purple), BFD (yellow), CEAS (blue), POX (pink) and PDC (red) together with some...

Ngày tải lên: 07/03/2014, 12:20

... shifts for DMT1-TM4 in TFE and SDS. Ó FEBS 2004 Structure and topology of TM4 of DMT1 (Eur. J. Biochem. 271) 1951 Structure and topology of the transmembrane domain 4 of the divalent metal transporter ... binding of peptides to membranes [37,45,46]. The exchange rate of the amide protons of DMT1-TM4 in both TFE and SDS micelles was monitored by both 1D and 2D 1 H...

Ngày tải lên: 16/03/2014, 16:20