Báo cáo khoa học: MBP-1 is efficiently encoded by an alternative transcript of the ENO1 gene but post-translationally regulated by proteasome-dependent protein turnover doc
Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt
... translational motion of spherical molecules in solution and the hydrodynamic radius is governed by the equation, D = K B T ⁄ 6pag, where g is the solvent viscosity and a is the radius of the molecules. The diffusion ... the conforma- tion of the host protein CD2 is largely unchanged. Additional resonances appearing between 8.2 and 8.8 p.p.m. were caused by...
Ngày tải lên: 07/03/2014, 00:20
Tài liệu Báo cáo khoa học: Hepatic stimulator substance mitigates hepatic cell injury through suppression of the mitochondrial permeability transition pdf
... TE (1994) Cloning and sequence-analysis of the rat aug- mentor of liver-regeneration (ALR) gene: expression of biologically-active recombinant ALR and demonstra- tion of tissue distribution. Proc ... from the generation of an electro- chemical gradient across the IM forms the basis of the IM w m . During cell death, MP often increases, allow- ing for the release of...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: Arabidopsis thaliana BTB⁄ POZ-MATH proteins interact with members of the ERF⁄AP2 transcription factor family ppt
... cotyledons and in the central cylinder of the root, but not in the lower parts of the hypocotyl (a). Rosette leaves were stained in the vascular tissue of the leaf blade, whereas the petiole and older ... factor in planta, it is still unclear whether BPM and RAP2.4 genes are expressed in the same tis- sues. Consequently, we analyzed the tissue-specific expression pa...
Ngày tải lên: 18/02/2014, 06:20
Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc
... identified by GC-MS analysis, and the determination of released ammonia. The coupled enzyme assay revealed the mechanism of the deamination reaction and the subsequent metabolism, including the deamination step. The ... (A) the fluorescence excitation spe ctrum (detect ed at 530 nm) and ( B) the emission spe ctrum (excited at 450 n m) of the supernatant of the he...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx
... The absence of redox centres (FAD, Fe/S) excludes the first hypothesis and therefore a direct involvement of PHI in electron transfer. This conclusion is consistent with the very low degree of identity ... and 18 kDa by gel-permeation. This finding suggests that the protein in its native state is a homodimer. The isoelectric point is 4.1. PHI does not contain any redox...
Ngày tải lên: 21/02/2014, 00:20
Báo cáo khoa học: UDP-galactose 4-epimerase from Kluyveromyces fragilis – catalytic sites of the homodimeric enzyme are functional and regulated docx
... from MS analysis and the kinetic lag in catalysis that the inhibi- tor remained associated with the enzyme in the pres- ence of low concentrations of UDP-Gal (Fig. 5). Analysis of the Michaelis–Menten ... are functional and are distinguishable on the basis of bind- ing with the inhibitor. Careful analysis of Fig. 2 shows that inactivation of E 3 by trypsin and redu...
Ngày tải lên: 16/03/2014, 00:20
Báo cáo khoa học: 2-Oxo acid dehydrogenase complexes in redox regulation Role of the lipoate residues and thioredoxin pot
... of the lipoate/dihydrolipoate couple of the complex (Fig. 3) and the intimate link between this couple and E1, the E1 dissociation from the complex to bind DNA may represent another form of the ... Thus, the lipoate residues of the complex mediate the regulation of its first component by the last product of the overall reaction, NADH. Reduction of the li...
Ngày tải lên: 17/03/2014, 09:20
Báo cáo khoa học: TioS T-TE – a prototypical thioesterase responsible for cyclodimerization of the quinoline- and quinoxaline-type class of chromodepsipeptides potx
... appending an C-terminal hexahisti- dine tag to the expressed protein. DNA sequencing of the derived plasmid was performed by GATC Biotech (Kon- stanz, Germany) on an ABIprism 310 genetic analyzer (Applied ... TL4, the analogous substrate TL10 was synthesized. HPLC-MS analysis revealed the formation of the macrocycle Cy10 at a hydrolysis to cyclization ratio of 8 : 1. Ad...
Ngày tải lên: 23/03/2014, 06:20
Báo cáo khoa học: Succinate dehydrogenase flavoprotein subunit expression in Saccharomyces cerevisiae – involvement of the mitochondrial FAD transporter, Flx1p ppt
... [52]. PCR analysis confirmed replacement of the stop codon of the SDH1 ORF by the 3xHA-loxP-kanMX- loxP cassette in both the G418-resistant transformants, EBY157-SDH1-HA (WT-HA)andEBY167-G418 S -SDH1- HA(flx1D-HA). Construction ... compartment. After 5 h of growth, the intensity of the 70 kDa band increased two-fold, and this increase was prevented by FCCP (Fig. 4A, lane...
Ngày tải lên: 23/03/2014, 07:20
Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf
... NirF and NirS; it is the His residue that in NirS is the proximal axial ligand to the d 1 heme. Replacement of an equivalent His, His41, in NirF by Ala abolished binding of the heme to the protein. Known ... Medicine, University of Oxford is thanked for her assistance with the MS of the proteins. Amy Varney and Christopher Greening are thanked for con- struct...
Ngày tải lên: 15/02/2014, 01:20