Báo cáo khoa học: Aspergillus nidulans a-galactosidase of glycoside hydrolase family 36 catalyses the formation of a-galacto-oligosaccharides by transglycosylation doc
Báo cáo khoa học: Aspergillus nidulans a-galactosidase of glycoside hydrolase family 36 catalyses the formation of a-galacto-oligosaccharides by transglycosylation doc
... Journal compilation ª 2010 FEBS 3551 Aspergillus nidulans a-galactosidase of glycoside hydrolase family 36 catalyses the formation of a-galacto-oligosaccharides by transglycosylation Hiroyuki Nakai1, ... analysis of Thermotoga maritimaGH36 a-galactosidase (TmGalA) confirms the mecha-nistic commonality of clan GH-D glycoside hydrolases.Biochemistry 46, 3319–3330.25 Zechel DL & Withers SG ... at the anomeric centre. In the next step,water is deprotonated by the general base catalyst andattacks the anomeric centre, releasing the carbohydratemoiety. For GH36, the nucleophile and the...
- 14
- 579
- 0
Báo cáo khoa học: "Parsing with Treebank Grammars: Empirical Bounds, Theoretical Models, and the Structure of the Penn Treebank" ppt
... NN:[2,3])3.1 Time The parser has an theoretical time bound,where is the number of words in the sentence to beparsed, is the number of nonterminal categories in the grammar and is the number of (active) ... shows the effect of the tree transforms ontraversal counts. The NOUNARIES settings are muchmore efficient than the others, however this efficiencycomes at a price in terms of the utility of the ... times in figure 3. The interesting cause of the varying exponentscomes from the “constant” terms in the theoreticalbound. The second half of this paper shows howmodeling growth in these terms can...
- 8
- 404
- 0
Báo cáo khoa học: Human ATP-dependent RNA ⁄ DNA helicase hSuv3p interacts with the cofactor of survivin HBXIP ppt
... in the truncated protein levelswithin 4 h following inhibition of protein synthesis by cycloheximide. We next wanted to examine whether the presence of HBXIP binding protein fragment of the hSuv3 ... restoration of apoptotic ability of cancer cells.To the best of our knowledge no other interactors of the HBXIP have been reported, nor has its exactABCFig. 4. Expression of hSuv3p lacking the HBXIP ... encode the HBXIP protein. The results of our two-hybrid screen have been confirmed by pull-down assays.We constructed a series of deletions of the hSUV3cDNA and demonstrated that only the 136 amino...
- 12
- 468
- 0
Báo cáo khoa học: Affinity purification-mass spectrometry Powerful tools for the characterization of protein complexes ppt
... provided by affinity-based methods. The commontheme of these is the use of an inherent interaction (affinity) of two biomolecules. If one of the molecules is immobilizedon a solid support, the interacting ... in the sequence (Fig. 2). Quite often,only a part of the sequence can be read from the sequence.However, this stretch of consecutive sequence is ÔlockedÕwithin the peptide by the masses of the ... and that the results areusually straightforward to interpret. The downsides are the statistical limitations imposed by the method. Theseinclude the requirement that the majority of the codingsequence...
- 9
- 415
- 0
Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf
... Asp141 of Met8P. The idea of NirF being a dehydrogenase is appealingbecause of the presence of a putative nucleotide-bind-ing motif in the N-terminal of the protein sequenceand also because there ... NirF and a range of nucleotide-containing cofactors by using a variety of biophysical methods (data not shown). Nonetheless,we still decided to test the effect of the deletion of the entire GXGX2GX7G ... d1heme-bindingdomain of cytochrome cd1. Therefore, we tested for the binding of d1heme to purified NirF. The addition of d1heme to the NirF resulted in the appearance of adistinctive visible...
- 12
- 613
- 0
Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf
... surface of the cell that pro-duced them. The ECM can be found in differentforms, from the hard compositions of bone to the softstructures of connective tissue. Collagens are the maincomponents of ... independent of whether the fibrillar 3D collagen was attached to orreleased from plastic, or whether the cells wereattached on top of or inside the fibrillar 3D matrix(data not shown). Some of the synthesized ... as their acti-vation of MMP-2. In addition, it adds to the earlierinvestigations by showing that the structure of the sur-rounding matrix components will be of importance for the effect of...
- 12
- 572
- 0
Tài liệu Báo cáo khoa học: 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5¢-phosphate synthases of fungi and archaea docx
... chemical synthesis of the tracenutrient [9]. The biosynthesis of the vitamin is summarised inFig. 1. Although the final part of the pathway isuniversal in all organisms studied to date, the earlysection ... reduction of the ribosyl side chain of compound 3 in the ascomy-cete Ashbya gossypii is conducive to introduction of ahydrogen atom into the 1¢-proS position of the ribitylside chain of riboflavin ... cataly-sing the transfer of deuterium from the 4(R) position of NADPH to the 1¢ (S) position of the substrate.ResultsPyrimidine reductase familiesUsing the amino acid sequence of 2,5-diamino-6-ribi-tylamino-4(3H)-pyrimidinone...
- 12
- 550
- 0
Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
... accommodate the cisplatin IACs. The higher the probability of formation of the cisplatinIACs within the p53DBSs due to the occurrence of the GG, AG and ⁄ or GNG motifs, the stronger the inhibition of the ... treated with the drug in excess of nonspecific calf thymus DNA. Inter-action of the protein with any of these targets was sig-nificantly affected by the cisplatin treatment, but the levels of inhibition ... Like p53(1 363 ), the fl pro-tein was able to recognize sequence specifically all of the targets tested (not shown). The effects of the degree of DNA cis-platination on recognition of the particular...
- 14
- 597
- 0
Tài liệu Báo cáo khoa học: What makes biochemical networks tick? A graphical tool for the identification of oscillophores ppt
... each other). The value (the sign) of a cycle is the product of the values (the signs) of its steps. The sign o f the magnitude of a Ôminus combined autoinfluence path of order pÕ equal those of the ... The absolute magnitude of acombined autoinfluence path is the product of all the ratesdivided by the product of all the concentrations of itsspecies. Therewith the c oefficient of o rder p of ... follows. Theyindicate the influence the (production rate of the) substanceon the right may experience through reaction r,fromthesubstance o n the left, which is a substrate of that reaction r ifthe...
- 11
- 638
- 0
Tài liệu Báo cáo khoa học: 1,5-Diamino-2-pentyne is both a substrate and inactivator of plant copper amine oxidases ppt
... As in the case of DABY, the cofactor topaquinone is not modified by the reaction, as demonstrated by an unchanged quinone redoxstaining.Absorption spectroscopy demonstrated the formation of a ... active sites of the enzymes resulting in the preference for C5-diamine sub-strates by OVAO.Similarly to the conversion of its lower homolog DABY by PSAO [7], DAPY oxidation by the studied enzymes ... that of free ACA, showinga loss of a water molecule from the carboxylic group ()18,m/z 211.3 fi m/z 193.3).There are detailed reports on the mechanism-basedinhibition of CAOs by DABY in the...
- 13
- 604
- 0
Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinChuong 2 nhận dạng rui roQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)HIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMMÔN TRUYỀN THÔNG MARKETING TÍCH HỢPQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ