Báo cáo khoa học: Lpe10p modulates the activity of the Mrs2p-based yeast mitochondrial Mg2+channel pot

... expression on the size of Mrs2p-containing complexes (Fig. 6), we examined whether the presence of Lpe10p might affect the characteristics of the Mrs2p channel (e.g. its conduc- tance of  155 pS) ... prominent decrease in the mitochondrial membrane potential (DW). To obtain further insights into the diverse functions of Lpe10p and Mrs2p, we constructed Mrs2 -Lpe10...

Ngày tải lên: 29/03/2014, 21:20

... inhibitors of the host [16,18]. Full virulence of Yersinia depends on all of these properties mediated by YpkA, because mutations or deletions in either the kinase or GTP-binding domains reduce the ... Kessler Department of Clinical Medicine, University of Oxford, UK Introduction The genus Yersinia consists of three pathogenic species that are agents of a variety of di...

Ngày tải lên: 16/02/2014, 15:20

... l M of OsDmc1 in absence of ATP; (2) with 1.25 lM of OsDmc1 in presence of 2.0 m M of AMP-PNP; (3) with 1.25 lM of OsDmc1 in presence of 2.0 mM ATP-c-S; (4) with 1.25 lM of OsDmc1 in presence of ... either no nucleotide cofactor or had slowly hydrolysable analogues of ATP. The rate enhancement by the presence of ATP was in the order of about 15-fold (Fig. 6), str...

Ngày tải lên: 19/02/2014, 07:20

... respectively, of that of HPRT. This low activity could be explained by the fact that PRTFDC1 has a Gly in the position of the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at the position of ... and regulators of other enzymes in the human nucleotide metabolism (Table S1). The library can then be seen to constitute the specific metabolome of the n...

Ngày tải lên: 15/02/2014, 01:20

... because of the large size of some of the introns, they are not. For stat6, potential missing human exons in zebrafish are indicated by ‘*’. The EMBL accession numbers of the T-bet genes are as follows: ... zffoxp3-R2 for foxp3. These primers amplified part of the initial predicted sequence which contained the majority of the open reading frames of zebrafish stat6, t-b...

Ngày tải lên: 16/02/2014, 09:20

... respectively. Although the over- all number of these modifications is low, the density of modifications is much the same as in the rest of the lin- ker histones. It is the shortness of the N-terminal tails Table ... histones Historically, the location of the globular domain of the linker histone has been a matter of contention [13]. Currently, although there is...

Ngày tải lên: 18/02/2014, 08:20

... functions. The gene encoding the cyclin-depen- dent kinase inhibitor p21 is a target gene of SREBP [20]. This finding suggests that the regulation of lipid synthesis is linked to the regulation of cell ... involved in the regulation of genes participating in cholesterol biosynthesis and low-density lipoprotein receptor synthesis [1,2]. They are now estab- lished as global reg...

Ngày tải lên: 18/02/2014, 13:20

... centrifugation of the samples, proteins bound to liposomes were analyzed by SDS ⁄ PAGE of the resulting pellets. (C) The amounts of I C in the pellets. The amounts of I C were quantitated with the UN-SCAN-IT ... the fluorescence of I C –GFP was observed at the FM4-64- stained vacuolar membranes and also the vacuolar lumens in the majority of yeast cells (70%...

Ngày tải lên: 19/02/2014, 05:20

... and phylogeny. How- ever, the nature of the connections between these factors needs to be understood at the level of the pro- tein domain. The global properties of the HTFN topology are partially ... of interacting proteins responsible for the control of gene expression. They interact with DNA regions and tend to form transcriptional regulatory complexes. Thus, the fi...

Ngày tải lên: 19/02/2014, 07:20

... cant lipid mixing activity, demonstrating the specificity of the activity of the IFP–wt sequence. Inspection of Fig. 3 revealed that lipid mixing activity is higher for IFP–wt than the N-terminal fusion ... polarized light, respectively. The bottom panel displays the result of deconvolution of the IR absorption spectrum of the peptide f or analysis of the s...

Ngày tải lên: 19/02/2014, 16:20