Báo cáo khoa học: Inhibitory activity of double-sequence analogues of trypsin inhibitor SFTI-1 from sunflower seeds: an example of peptide splicing docx

... Inhibitory activity of double-sequence analogues of trypsin inhibitor SFTI-1 from sunflower seeds: an example of peptide splicing Anna Łe˛ gowska 1 , Adam Lesner 1 ,El _ zbieta Bulak 1 , Anna ... x10 8 [Phe ]SFTI-1- chymotrypsin complex 5 A B Fig. 4. MS spectra of (A) bovine a-chymotrypsin and (B) a mixture of a-chymotrypsin and [FF]BiSFTI-1 (6). A. Łe˛ go...

Ngày tải lên: 29/03/2014, 09:20

... 241–246. 49 Huang XM, Bennett M & Thorpe PE (2004) Anti-tumor effects and lack of side effects in mice of an immunotoxin directed against human and mouse prostate-specific membrane antigen. Prostate ... Kreitman RJ & Pastan I (1998) Immunotoxins for targeted cancer therapy. Adv Drug Deliv Rev 31, 53–88. 48 Lin J, Yan F, Tang L & Chen F (2003) Antitumor effects of curcin fr...

Ngày tải lên: 07/03/2014, 16:20

... Germany 2 Research Group Modeling of Biological Systems, German Cancer Research Center and BioQuant Center, Heidelberg, Germany 3 Department of Parasitology, Berlin, Germany 4 Institute of Pathology, ... many cell types. A myr- iad of stimuli can activate these kinases, which, in turn, activate many other transcription factors and regulators of transcription, controlling the express...

Ngày tải lên: 29/03/2014, 23:20

... phenylmethanesulfonyl fluoride and calpain inhibitor I (ALLNal) were obtained from Nacalai Tesque (Kyoto, Japan). Antipain and E-64 were obtained from the Peptide Institute (Osaka, Japan). Pefabloc and ... staining. Open triangles and closed triangles indicate bands of recombinant calpain 7MIT and GST, GST–IST1, GST–IST1MIM, and GST–IST1MIM L326D,L353A , respectively. Enhancement of...

Ngày tải lên: 18/02/2014, 04:20

... the membrane of cells. Inhibitory effects of VVA1 on the hemolytic and cytotoxic activity of VVA2 The effects of VVA1 on the hemolytic activity of VVA2 were studied by incubation of human red blood cells ... as diphtheria and anthrax toxins, as well as the less well- known a-hemolysin and equinatoxin II [11–16]. An important objective is to provide an effective inhibito...

Ngày tải lên: 19/02/2014, 06:20

... level of Xantha-g mRNA. Therefore, the presence of Xantha-g mRNA was analysed in one semidominant and one recessive xantha-h mutant (Xantha- h clo 157 and xantha-h 57 , respectively). First strand ... that the XAN-G protein was missing in total cell extracts of the xantha-h 56 and xantha-h 57 mutants [33]. The two mutant plants are suggestedtolackexpressionoftheXAN-Hproteinasno Xantha-h...

Ngày tải lên: 19/02/2014, 12:20

... a4b2 activity. This was determined by analysis of a truncated analogue of GID ([D1–4]GID) that displayed no significant change in activity for the a7anda3b2 receptor subtypes [16], but a4b2 activity ... in a range of a-conotoxins. In addition, an alanine scan of the non-cysteine residues has been performed on PnIB [38]. Extensive mutational analysis has been carried out on ImI,...

Ngày tải lên: 19/02/2014, 12:20

... of cyst- atin F for these enzymes. Proline in the S 2 site is a fea- ture of human stefin A and cystatins F, S and SN, all of which are significantly less potent inhibitors of cath- epsin B than ... cystatin F is shown, from 0% (uninhibited enzyme) to 100% (the lowest activity of the enzyme). Dimerization of cysta- tin F leads to a loss of inhibitory activity. (C) Immunoblo...

Ngày tải lên: 19/02/2014, 17:20

... structure and mechanism of catalysis. Annu. Rev. Biochem. 46, 331–358. 43. Blow, D.M., Birktoft, J.J. & Hartley, B.S. (1969) Role of a buried acid group in the mechanism of action of chymotrypsin. ... pH variable from 5.0 to 8.5 and, in 0.1 M sodium acetate buffer, pH ranging from 4.0 to 5.25. The ionic strength of phosphate buffers varied from 0.1 to 0.29, and that of a...

Ngày tải lên: 20/02/2014, 23:20

... Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifu- gation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand. ... it possesses significant lower activity than enterobacterial LPS in terms of pyrogenicity or Schwartzman-reactivity in rabbits, lethality in galactosamine-sensitized mice, anti- c...

Ngày tải lên: 20/02/2014, 23:20