Báo cáo khoa học: Physiological truncation and domain organization of a novel uracil-DNA-degrading factor pdf
... Drosophila melanogaster uracil-DNA-degrading factor; MQAP, model quality assessment program; TcUDE, Tribolium castaneum truncated uracil-DNA-degrading factor isoform; UDE, uracil-DNA-degrading factor; ... EMSA DNA cleavage assay Quaternary structure by gel flitration Theoretical analysis Experimental analysis Domain organization Analytical ultracentrifugation Circular dichroism...
Ngày tải lên: 29/03/2014, 08:20
... TSVSAGDGAFGNLAAALTLVEDTEDGLGVKTKNGGKGFSEGTAAISQTAGANGGATVKKA Bacp19k VSASAANGFFKNLGKATTEVKTTKDGTKVKTKTAGKGKTGGTATTIQIADANGGVSEKSL Bicp19k AAAAAGNGVFKNLVTALTNISTTDDITKVQTQTIGSGGTGGAATILQLADANGGAALKEV 130 ... Mountain View, CA, USA), and poly (A) + RNA was isolated using Oligo(dT)-Latex Super (Takara Shuzo Co.). cDNA was prepared from mRNA with a Zap-cDNA synthesis kit (Stratagene, La Joll...
Ngày tải lên: 16/03/2014, 05:20
... 275–283. 10 Kuwada M, Teramoto T, Kumagaye KY, Nakajima K, Watanabe T, Kawai T, Kawakami Y, Niidome T, Sawada K, Nishizawa Y et al. (1994) Omega-agatoxin- TK containing D-serine at position 46, but ... crosspeaks were assigned and inte- grated, with concomitant cycles of structure calcula- tions for evaluation of distance and angle constraint violations as well as assignments of add...
Ngày tải lên: 18/02/2014, 17:20
Tài liệu Báo cáo khoa học: Purification and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt
... ATCCTCACGAACAAGCAG 5RACR GATCGCGATGCAGGCCTT FLF1 GGACGACTACAGCGTCTTCAGTAGA FLR1 TCCAAACAGTCAGTTTCTTAACCGT Table 1. Purification of cellulase from abalone Haliotis discus hannai. Oneunitofcellulasewasdefinedastheamountofenzymethatliberates reducing ... RTARAAYTGNCCNGCRTCYTG LP6 WAVEQMNYILGDNK R2 CATYTGYTCNACNGCCCAYTT LP7 AWAWALGWDDK LP8 GYHENA LP9 WPLDYFL F2 GCCACACTTCTGTCAACATCC 3RAC TTCTTCAA...
Ngày tải lên: 20/02/2014, 23:20
Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt
... temperature until all free water was evaporated. After this, IR spectra were recorded at room temperature and at 37 °C. Usually, the original spectra were evaluated directly and a spectral analysis ... Chicester. 32. Mu ¨ hlradt, P.F. & Frisch, M. (1994) Purification and partial bio- chemical characterization of a Mycoplasma fermentans-derived substance that activates macrophag...
Ngày tải lên: 21/02/2014, 00:20
Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf
... chitinases have catalytic domains that are at least as large as those of the plant enzymes and that may contain at least six subsites [26,30]. However, the catalytic domains of ChiG, ChiC and most other ... has a similar domain structure to ChiC, consisting of a catalytic domain and an N-terminal chitin-binding domain. ChiG, on the other hand, lacks this chitin-binding d...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... 216 73TTREEQPAAEAAASTAGGSQ ACC ACC AGG GAA GAA CAA CCG GCG GCA GAG GCC GCG GCG TCC ACC GCT GGC GGT AGC CAA 276 93QEGYGSTQLPSDEPLNGLND CAA GAA GGA TAT GGC AGC ACC CAA CTT CCA TCG GAC GAG CCA TTG AAC GGG ... 636 213WKDTTSVGCGRVLCDDKKDT TGG AAG GAC ACC ACC AGC GTC GGA TGC GGA CGC GTC CTC TGC GAC GAC AAG AAG GAC ACC 696 233MIMCSYWPPGNYENQKPY ATG ATC ATG TGC AGC TAC TGG CCG CCG GGC AAC TAT GAA AAC...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Identification and functional expression of a second human b-galactoside a2,6-sialyltransferase, ST6Gal II docx
... HepG 2 cDNA library (C. Baisez and A. Harduin-Lepers, unpublished data), as the template and two specific primers For 6I 5¢-CGATGAATTC GTTAACGCTCATCACCATCACCATCACGGGAAA TTGGCCATGGGGT-3¢ containing a HpaIsiteandBack 6I ... kidney, thymus, liver), and rather weakly in placenta, lung, aorta, amygdala, occipital and parietal lobe and salivary gland. Almost no expression was observed i...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: Ligand binding and antigenic properties of a human neonatal Fc receptor with mutation of two unpaired cysteine residues docx
... half-life of IgG and albumin [1–3], is a major histocompatibility complex (MHC) class I-related receptor consisting of a heavy chain (HC) with three ectodomains (a1 , a2 and a3 ), a transmembrane ... variants. Table S3. Numbers of cysteine residues of FcRn HCs across species a . Table S4. Numbers of cysteine residues of nonclassical MHC class I HC a . This material...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx
... 5¢-CCTGATCGACGCACGAGT-3¢ and GT1-R, 5¢-TTTTGCAAGTCATAGTAATCAGTTT-3¢ for GT- cDNA1 (2150 bp); and GT2-F, 5¢-CACCAGCAACTAC CTGATCGA-3¢ and GT2-R, 5¢-CACAAAATATGCTT CCAAGTGC-3¢ for GT-cDNA2 (3043 bp). Construction and ... 3¢-RACE were: Primer C, 5¢-CCAGTGTCCCCAT CATCAG-3¢ and Primer D, 5¢-GGCAATTTTAAA GTCATTATGGCGCAAA-3¢. First strand cDNAs were synthesized using Superscript II RNase H...
Ngày tải lên: 17/03/2014, 03:20