Báo cáo khoa học: Role of the plasma membrane leaflets in drug uptake and multidrug resistance ppt

... December 2009) doi:10.1111/j.1742-4658.2009.07555.x The present study aimed to investigate the role played by the leaflets of the plasma membrane in the uptake of drugs into cells and in their extrusion by P-glycoprotein and multidrug resistance- associated ... accu- mulation of dye in the cytoplasmic leaflet of the plasma membrane and release...

Ngày tải lên: 29/03/2014, 08:20

... glycoprotein [28,29], in uenza virus hemagglutinin [30,31], and IgM [32] in ER, a further role of N-linked oligosacharide chains of fhx/P25 in the process of assembly of the elementary unit in ER ... assembly of the elementary unit of fibroin in ER It was demonstrated in this study that the elementary unit of fibroin, having the same molar ratio of H- a...

Ngày tải lên: 16/03/2014, 16:20

... tubulin partitioning into the detergent phase (HAT) is the acetylated tubulin forming a complex with H + -ATPase. The finding of the H + -ATPase–acetylated tubulin complex in plasma membranes of ... comparison of blots, it can be seen that most of the acetylated tubulin and ATPase were involved in the immunoprecipitation process. By measuring the intensity of t...

Ngày tải lên: 23/03/2014, 11:20

... an 8 0-fold increase in laccase produc- tion. The identity of the recombinant protein was further confirmed by immunodetection using Western b lot analysis and N -terminal sequencing. The m olecular ... strain ss3 of P. cinnabarinus. In order to improve the secretion of the recombinant laccase, the laccase cDNA was fused to the GLA prepro- sequence and the product...

Ngày tải lên: 17/03/2014, 11:20

... towards the flavin cofactor is possible only in the dimeric structure of NADH oxidase. None of the tryptophan residues is close to the binding site of the flavin cofactor in the monomeric form of the ... nitrogen of Trp47 and to O2 of the ribityl chain and is located in the middle of the flavin system with distances of 3.3 A ˚ toN1 0and3 .7A ˚ to N5....

Ngày tải lên: 23/03/2014, 15:21

... control the amount of intracellu- lar CagA PY . Cortactin, ezrin and vinculin are all Src substrates, and Src inactivation causes dephosphoryla- tion of these proteins and is crucial for triggering ... distribution, making them unsuitable for the investigation of b-cate- nin signalling [29]. In support of this aspect, using Madin–Darby canine kidney cells that express E-ca...

Ngày tải lên: 14/02/2014, 19:20

... motif Arg40 Cys84 Tyr349 Tyr347 Lys370 Ala36 Ala36 Ala36 Val357 Phe518 B Laccase Tyrosinase β -sheet protein Fig. 1. Overview of the tyrosinase gene and surrounding genes in the genome of V. spinosum. (A) Showing the tyrosinase gene and those in its immediate vicinity in the ... binding motifs common to most tyrosinase sequences [3] that contain five of the six coppe...

Ngày tải lên: 06/03/2014, 11:20

... which catalyzes the production of ROS and results in efficient killing of invading pathogens [5]. The effect of the calmodulin inhibitor W7 on fMLP induced ROS production was investigated and compared with the ... counteracts the calcineurin induced affinity switch of a 5 b 1 integrin in CHO cells, suggesting also a role for Ca 2+ /calmodulin-dependent molecules in...

Ngày tải lên: 08/03/2014, 10:20

... of the active site was better in the protein bear- ing the N2 domain than in the protein bearing the Nc domain. Thus, the accessibility of the active site in the N2 proteins was independent of ... of the water–lipid interface. Also, both the N-terminal and C-terminal domains of hoPL were involved in the inactivation of the protein at the water–t...

Ngày tải lên: 16/03/2014, 06:20

... Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase Carmine Ercole 1 , Francesca Avitabile 1 , Pompea Del Vecchio 1 , Orlando ... result also provides indirect evidence of correct pairing of the inter- chain disulfide bridges in the recombinant sample [11]. The kinetics of the N-terminal arms sw...

Ngày tải lên: 16/03/2014, 23:20