Báo cáo khoa học: Biochemical evidence for conformational changes in the cross-talk between adenylation and peptidyl-carrier protein domains of nonribosomal peptide synthetases ppt

... holo -protein. These results provide the first biochemical evidence for the occurrence of conformational changes in the cross-talk between A and peptidyl carrier protein domains of a multi- domain nonribosomal ... 2010 The Authors Journal compilation ª 2010 FEBS 1171 Biochemical evidence for conformational changes in the cross-talk between...

Ngày tải lên: 29/03/2014, 08:20

... (skeletal and heart muscle) and a 3 -isoform (nerve tissue and brain) are present. The exception is the kidney where more than 99.9% of the enzyme consists of the ouabain-insensitive a 1 -isoform ... -insensitive a-isoforms of Na + /K + -ATPase in rats All isoforms of the catalytic a -peptide of Na + /K + -ATPase have similar, high affinity for ouabain [17] with...

Ngày tải lên: 23/03/2014, 17:21

... Ôclosed-to-openÕ conformational change of TFIIB upon binding to the TBP– DNA complex, which probably involves alternation of the spatial orientation between the N-terminal zinc ribbon domain and the C-terminal ... t and at in nity, respectively. An error bar indicates the SD of each data point from the average value. Results Design and biochemical integrity of...

Ngày tải lên: 07/03/2014, 15:20

... c 10 complex in the recombinant c ring preparations suggests that the insertion of the last c subunit forms the limiting step in the assembly process of a functional oligomer. Upon closer inspection, the ... striking feature of the structure is an inherent asymmetry among the catalytic b subunits. In combination with the asymmetric loca- tion of the central c...

Ngày tải lên: 07/03/2014, 21:20

... domains (Fig. 1). These types of interactions are observed either between residues located in blades IV and V and the C-terminal domain or between the hair- pin that joins blades IV and V and the ... for the optimal activity of BsArb43B. Furthermore, to determine whether the role of the calcium is structural, thermal shift assays were per- formed to determi...

Ngày tải lên: 15/03/2014, 23:20

... structure exists in the sentence labels, the document collection was randomly split into training and test sets. The au- tomaton produced by MDI was learned using the training data, and the probability ... calculated the %F1 achieved by the average-link variation of the method for varying levels of training data. Over- all, an improvement of approx. 5% is observe...

Ngày tải lên: 20/02/2014, 12:20

... to the string including errors from the String- Database (the former string is referred to as the Similar-String, and the latter as the Error-String). Finally, the correction is made using the ... value for the frequency of the occurrence, and 10 as the length of a string, therefore obtaining 16655 strings. 3.2 Two Factors for Evaluation We evaluated th...

Ngày tải lên: 20/02/2014, 18:20

... French According to our survey, 1988 sales of mineral water and soft drinks were much higher than in 1987, reflecting the growing popularity of these products. Cola drink manufacturers in particular ... rate. By selecting the best scoring 80% of the alignments, the error rate is reduced from 4% to 0.7%. There were roughly the same number of errors in each o...

Ngày tải lên: 20/02/2014, 21:20

... performed with a standard protocol using the biotin–avidin-based Vectastain Elite kit (Vector Laboratories) and diamino- benzadine (DAB) for detection of the secondary anti- body, according to the ... Tissue-specific expression of mast cell granule serine proteinases and their role in inflammation in the lung and gut. Immunol- ogy 105, 375–390. 5 Caughey G (2001) New dev...

Ngày tải lên: 07/03/2014, 11:20

... cause -of “because of Another point to be mentioned is a delicate semantic difference between the meaning of these nouns in other constructions and in combination with prepositions. For ... functions in Farsi and at the same time very important roles in linguistics especially in computational linguistics. Most of the linguists consider them as members...

Ngày tải lên: 08/03/2014, 02:21