Báo cáo khoa học: Enhanced sensitivity to hydrogen peroxide-induced apoptosis in Evi1 transformed Rat1 fibroblasts due to repression of carbonic anhydrase III docx

... Journal compilation ª 2009 FEBS Enhanced sensitivity to hydrogen peroxide-induced apoptosis in Evi1 transformed Rat1 fibroblasts due to repression of carbonic anhydrase III P. Roy 1 , E. Reavey 1 , ... H 2 O 2 -induced apoptosis in Rat1 cells expressing an Evi1 transgene. Surprisingly, we found that Evi1 expression increases sensitivity to H...

Ngày tải lên: 29/03/2014, 08:20

... of the enzyme [7]. Having previously cloned the mph gene from Ochrobactrum sp. M231 [7], we sought to increase the thermostability of this MPH using pro- tein engineering. The two main protein-engineering ... September 2010) doi:10.1111/j.1742-4658.2010.07895.x Protein thermostability can be increased by some glycine to proline muta- tions in a target protein. However, not all glyci...

Ngày tải lên: 15/02/2014, 01:20

... expression of G L and PTG, but not R6, in rat liver in vivo is insulin-dependent. It declines during insulin deficiency and is restored by insulin treatment [22,40]. Another glycogen-targeting protein expressed ... glucose- induced insulin secretion and insulin resistance in the liver and periphery. Hepatic insulin resistance is attrib- uted to a range of metabolic defects, which i...

Ngày tải lên: 30/03/2014, 11:20

... release zinc from zinc-binding proteins The effect of aldehydes on the zinc-binding capacity of zinc proteins was assayed by employing spectropho- tometry and the chromophoric indicator 4-(2-pyridyl- azo)-resorcinol ... cysteine ligands of zinc sites in proteins and concomitant zinc release. Stoichiometric amounts of acrolein release zinc from zinc–thiolate coordination sites in p...

Ngày tải lên: 19/02/2014, 06:20

... each dataset into 10 subsets where each subset was a test set and the corre- sponding 9 remaining sets were merged to a train- ing set. We kept the labels of the training set to determine model ... our experimental setup. Instead of dividing datasets during 10-fold cross-validation into training and test subsets with the ratio of 9:1 we randomly split the data into training, validat...

Ngày tải lên: 20/02/2014, 04:20

... eosin staining. We examined the expres- sion of these proteins in a cancer-free layer of squamous epithelial cells, carcinoma in situ (CIS) and invasive cancer, all of which were observed in ... proteins bound to chromatin probably function in DNA replication, but the role of the proteins not tightly bound to chromatin remains to be determined. Consistent with the result...

Ngày tải lên: 20/02/2014, 23:20

... amino acid homocysteine both in vitro and in vivo. AS101 conferred protection against homocysteine-induced apoptosis of HL-60 cells. The protective mechanism of AS101 against homocysteine toxicity ... homocysteine to form homocystine, the less toxic disulfide form of homocysteine. Moreover, AS101 was shown here to reduce the levels of total homocysteine in an in vivo mode...

Ngày tải lên: 07/03/2014, 09:20

... deubiquitinating enzymes, mainly consisting of cysteine proteases [43]. Another class of proteins are the tail-anchored proteins, which include the translocases of the mitochondrial outer membrane, Tom5, ... Biochemicals, Stockton, UK) as substrate, 20 lL yeast extract, various inhibitors and activators in a total volume of 30 lL. The mixture was incubated at 30 °C f or 2.5 min to...

Ngày tải lên: 07/03/2014, 16:20

... that single-chain HGF was inactive in the presence of serine protease inhibi- tors in a hepatocyte proliferation assay, whereas the two-chain form was active even in the presence of the inhibitors ... next administered hepatotoxin or nephrotoxin to rats to induce tissue injury. HGF was extracted from injured and uninjured tissues and then analyzed. After intraga- stric administr...

Ngày tải lên: 15/03/2014, 11:20

... with an increase in the levels of several FAA during the first days in most species tested to date, resulting in an increase of the total FAA pool [13,19]. Even though no significant increase of the ... adults of the beetle Alphitobius diaperinus in order to determine whether FTRs trigger the initiation of a metabolic response involving synthesis of protective compounds,...

Ngày tải lên: 16/03/2014, 11:20