Báo cáo khoa học: ERK and cell death: Mechanisms of ERK-induced cell death – apoptosis, autophagy and senescence doc

... reactions essential for growth and proliferation, is uncertain. Hence, it is of interest to investigate the uptake and metabolism of NTBI in normal and cancer cells, and the effect of Fe chelators on these ... overload conditions, and is also implicated in intracellular Fe metabolism [3,9,10]. An important aspect of this work was the assessment of the effect of novel Fe...

Ngày tải lên: 20/02/2014, 11:20

... to indicate the presence of the specific holocytochrome under investigation if the features of the spectrum, in terms of wavelength maxima, and the position and intensity of heme-staining bands on SDS-PAGE ... heme (saturation of each heme vinyl group on formation of a c-type cyto- chrome causes a blue shift of 2–3 nm in the a-band of the absorption spectrum). To confirm t...

Ngày tải lên: 06/03/2014, 09:22

... inhibited the proliferation of MCF-7 cells and induced cell death. (A) MCF-7 cells were treated with different concentrations of curcumin for one cell cycle and the inhibition of cell proliferation was ... 1C,D). For example, 4 and 24% of MCF-7 cells showed nuclear localization of p53 (Fig. 1C), whereas 7 and 33% of cells showed nuclear localization of p21 in the...

Ngày tải lên: 23/03/2014, 03:20

... using a PerkinElmer L S-50 fluorimeter at room temperature with a scan speed of 500 nmÆmin )1 and a n excitation wavelength of 280 nm, and scanned over the range 30 0–4 50 nm. An accumulation of eight ... this method contained a mixture of proteins B and B¢.The relative abundance of p roteins B and B¢ was assessed b y using SDS/PAGE and electrospray ionization (ESI)-MS. I...

Ngày tải lên: 31/03/2014, 09:20

... in the absence of sulfide, but artificial incorporation of Fe 3+ was not detected (not shown). The presence of iron–sulfur clusters was investigated by quantification of nonheme iron and acid-labile ... Lake, Michigan, USA) containing an atmosphere of 97% N 2 and 3% H 2 . Cells were lysed with B-Per (Pierce, Rockford, IL, USA), and detergent and cell debris were removed by ce...

Ngày tải lên: 28/03/2014, 23:20

... Differ 16, 36 8–3 77. 6 Subramaniam S & Unsicker K (2009) ERK and cell death: ERK1 ⁄ 2 in neuronal death. FEBS J. 7 Teixeiro E & Daniels MA (2009) ERK and cell death: ERK location and T cell selection. ... 59, 7 6–8 6. ERK and cell death S. Cagnol and J C. Chambard 20 FEBS Journal 277 (2010) 2–2 1 ª 2009 The Authors Journal compilation ª 2009...

Ngày tải lên: 29/03/2014, 08:20

... 2-implicated cell death paradigms. ERK1 ⁄ 2 in cellular models of neuronal death The first evidence for a role of ERK1 ⁄ 2 in cell death was demonstrated in the oligodendroglial cell line, CG4. ... plasma membrane damage and independently of caspase-3. J Cell Biol 165, 35 7–3 69. 35 Mebratu Y & Tesfaigzi Y (2009) How ERK1 ⁄ 2 acti- vation controls cell prolife...

Ngày tải lên: 29/03/2014, 08:20

... MINIREVIEW ERK and cell death: ERK location and T cell selection Emma Teixeiro and Mark A. Daniels Department of Molecular Microbiology and Immunology, Center for Cellular and Molecular Immunology, ... activity and subcellular location are the same regardless of the ligand strength, whereas ERK activity and location change depending of the nature of the sele...

Ngày tải lên: 29/03/2014, 08:20

... the result of cell death and not due to an inhibition of cell proliferation, as determined by cell counting as well as quantification of loss of cell integrity [22]. Although the ERK prodeath effect ... protection against cell death by apoptosis, it is now clear that ERK can also be directly linked to cell death signaling. Here, we review the role of ERK i...

Ngày tải lên: 29/03/2014, 08:20

... 3′UTR pri-23a pcDNA3 miR-23a ASO ASO-NC + – – – – – – + – – – – – + + – – – – + – + – – – + – – + – – + – – – + 2833 2843 3123 3133 Fig. 3. IL6R is a direct target of miR-23a. (A) The IL6R 3¢ UTR carries ... promotes growth activity of MGC803 cells. MGC803 cells were transfected with miR-23a ASO or pri-23a v...

Ngày tải lên: 18/02/2014, 04:20