Báo cáo khoa học: Ion channel activity of brain abundant protein BASP1 in planar lipid bilayers pptx

... Ion channel activity of brain abundant protein BASP1 in planar lipid bilayers Olga S. Ostroumova 1 , Ludmila V. Schagina 1 , Mark I. Mosevitsky 2 and Vladislav V. Zakharov 2 1 Laboratory of Ionic ... bilayers. Results BASP1 exhibits single channel- like activity on planar lipid bilayers The activity of BASP1 and GAP-43 proteins in nega- tively-ch...

Ngày tải lên: 28/03/2014, 23:20

... doxycycline-inducible protein expression in human cell lines by just a single transfec- tion. For closely matching cellular backgrounds we use continuous expression of the original TetR instead of TetR-transactivator ... that in high numbers, even TetR fusion proteins based on VP16 minimal activation domains are toxic [12]. Thus, relocation of TetR fusion proteins upon binding of...

Ngày tải lên: 23/03/2014, 09:21

... accumulation of globin in MA-treated cultures (Fig. 1), indicating that the ini- tial hydrolysis of haemoglobin is not inhibited by MA. In addition, the morphology of infected erythrocytes incubated in ... pitrilysin [32], possibly involved in the processing of apicoplast protein leader sequence. Inhibition of any of these activities could contribute to MA interference...

Ngày tải lên: 14/02/2014, 14:20

... involving the co-ordination of the two distinct functions of TorD with the folding of TorA and possible interactions of both proteins with the MGD biosynthetic apparatus [12]. The concentration of ... hydrolysis activity is regulating the intra- and inter-molecular interactions exhibited by the TorD protein. ABC D G EF Fig. 8. Putative GTP binding regions of TorD family protei...

Ngày tải lên: 16/02/2014, 09:20

... binding to the protein was detected as an increase of fluorescence intensity in the p resence of p rotein compared to the fluorescence intensity in its absence [23,42]. Determination of eosin binding ... eosin binding to the fusion proteins Interaction of eosin Y with GST fusion proteins was studied in similarity to Skou & Esman [43] in 20 m M Tris/HCl, pH 7.8, at 37 °C. E...

Ngày tải lên: 19/02/2014, 16:20

... characterization of protein interac- tions. Protein protein interactions are distributed over a large surface area comprising many smaller local interactions. Advances in our understanding of these processes ... calcium ions is an emerging and interesting area for investigation. In view of these observations, interactions between the Ca 2+ -binding protein Calnuc and signaling...

Ngày tải lên: 07/03/2014, 00:20

... concentrations. The minimal lethal concentration was defined as the lowest peptide concentration capable of killing 99% of the cells in a suspension containing 10 6 CFUÆmL )1 . In addition, to determine ... optimized activity and selectivity; and (b) the mechanisms of action, especially in bacterial cells. In an effort of rationalization, a minimalistic approach was initiate...

Ngày tải lên: 23/03/2014, 17:21

... known to be protein protein and protein RNA interaction surfaces and the phos- phorylation of serines affects those interactions as well as interactions between RS domain-containing proteins [49,50]. ... conditions. It is tempting to suppose that the transient interaction of a SR kinase with a SAFB protein under certain cellular conditions leads to temporary inhibition of phosphory...

Ngày tải lên: 30/03/2014, 01:20

... conserved in Rio2 proteins are indicated in green text. (B) The structure of the flap in the hinge region. Residues of the hinge region are shown in green stick representation. Structure and activity of ... design of new classes of drugs. Protein kinases are known players in the regulation of cell cycle control, in addition to their role in a wide variety of cel...

Ngày tải lên: 30/03/2014, 20:20

... obtain CXRs after insertion of endotra- cheal tubes, intravenous lines and chest drains, but not after insertion of nasogastric tubes. In addition, CXRs were obtained in the case of worsening of ... ultrasound of the thorax in case of pleural effusion on the CXR, start or change in medication (diuretics); insertion of a pleural drain; and repositioning of devices in...

Ngày tải lên: 25/10/2012, 10:39