Báo cáo khoa học: Structural analysis ofN-glycans of the planarian Dugesia japonica ppt

... Likewise, these glycopro- teins are localized in lysosome, however, Man6-SO 4 accounts for the majority of the sulfated sugar. In conclusion, the results obtained provide evidence of the nature of the ... spectrometry for the analysis of monosulfated oligosaccharides. Carbohydr Res 304, 1–9. 32 Wheeler SF & Harvey DJ (2001) Extension of the in-gel release method...

Ngày tải lên: 23/03/2014, 15:20

... the steps in the biosynthesis of the oligosaccharide portion of the LPS molecules. The genes lpsA, lic2A and lgtC encode glycosyltransferase enzymes involved in the addition of the globoside ... corresponding to the loss of t-HepIII at m/z 1205.5 was further fragmented to give the ion at m/z 957.5 corresponding to the loss of an unsubstituted HepII. This confirmed...

Ngày tải lên: 23/03/2014, 21:20

... map. The diam- eter of the circles is proportional to the cube root of the detected amount of PA-glycans. The positions of standard PA-glycans are indicated by crosses labeled (a)–(g). The standards ... In contrast, if the planarian has unique and complex N-glycans, the view that the diversity of N-glycans does not correlate simply with the phylogenic process i...

Ngày tải lên: 28/03/2014, 23:20

... conformation of loop 99–107 (L2) in the A protomer as compared to its conformation in the B, C and D protomers of the tetramer. This loop forms the roof of the sugar binding pocket of MornigaM and ... gJRL. The size and shape of the carbohydrate-binding site of MornigaM and other JRL essentially depends on both the conformation of the three loops that delineate...

Ngày tải lên: 30/03/2014, 20:20

... advantage of p53 mutation analysis, and a unique feature of this database, is the availabil- ity of the residual activity of the majority of p53 mis- sense mutants. The biological activity of mutant ... of a severe mutant. At the other end of the spectrum, where we have low energy, there is 75% probability for the mutation to be nonsevere if the energy is 0.1...

Ngày tải lên: 18/02/2014, 11:20

... is required to fulfil the physical requirements of the membrane of the anammoxo- some. The presence of the ether-linkages in the mem- brane lipids (linking the lipids to the glycerol backbone) ... simi- lar to that of the mixture of thermal stabilization products of the [5]-ladderane fatty acid 7a apart from the fact that the m ⁄ z values of the molecula...

Ngày tải lên: 23/03/2014, 15:20

... presence of alternative splicing around the 5¢-end of exon 6 of Meis2 and Meis3 was tested by RT-PCR. The positions of molecular mass markers are shown to the left, and the size in base pairs of the ... However, the activity of the full-length Meis1a was not tested, and on the basis of our work we expect that its activity would be inhibited by the conserved Hth...

Ngày tải lên: 16/02/2014, 15:20

... obtained with the transition at posi- tions 1915 and 1917. These mutations had an effect on the formation of pseudouridine exclusively at the mutant position and did not affect either of the other two ... by Southern analysis. The rluD::cat strain had a full deletion of both rluD and yfiH, together with their annotated pro- moter sequences. The inserted cat cassette had a di...

Ngày tải lên: 18/02/2014, 16:20

... Given that the roles of these residues definitely confirm each other, the dif- ference in their significance might be attributable to the importance and ⁄ or necessity of the receipt of the phenol-hydroxyl ... [20]. Fig. 2. Structural environments of BPA in the ligand-binding pocket of the ERRc. The proximity of each amino acid residue (within a distance of 5 A ˚...

Ngày tải lên: 18/02/2014, 16:20

... each extremity of the polypeptide chain, must of necessity arise from contacts that are made outwith the active site of the enzyme. The effect of removal of the N-terminal extension The full N-terminal ... binding of this helical N-terminal seg- ment of IA 3 . The E + I « EI balance thus lies far to the right and the addition of further residues at the N-te...

Ngày tải lên: 19/02/2014, 00:20