Báo cáo khoa học: Bcl-2 and Bcl-xL play important roles in the crosstalk between autophagy and apoptosis pdf

... and apoptosis. Thus, Bcl-2 and Bcl-xL represent a molecular link between autophagy and apoptosis. Here, we discuss the possible roles of Bcl-2 and Bcl-xL in apoptosis and autophagy, and the crosstalk ... onhow Bcl-2 and Bcl-xL are involved in the molecularevents. The crosstalk between the autophagy and apoptosis may redeﬁne the roles of Bcl-2 and Bcl-xL in oncogenesis and tumor progression. It ... cancer treatment bymodulating the two processes. Bcl-2 and Bcl-xL in the apoptosis Bcl-2 and Bcl-xL inhibit apoptosis The Bcl-2 protein family was discovered by analysis of the t(14–18) chromosomal...

Báo cáo khoa học: Interactions of ultraspiracle with ecdysone receptor in the transduction of ecdysone- and juvenile hormone-signaling pdf

... receptors, including human RXR (which is the ortholog of USP) have shown that an area on the surface of the ligand binding domain involving the C-terminus of a-helix 3, a-helix 4, and the N-terminusof ... promoter reporter.These two motifs (DR1 and IR1) contain identical halfsites and the same intervening single base, the differ-ence being the orientation of the second half site as in the same direction ... The green ﬂuorescent protein (GFP)-dUSP fusionprotein was prepared in pIE1-4 by subcloning the GFPcoding sequence for into the EcoRI ⁄ SmaI sites of the pIE1-4 vector, upstream of and in the...

Báo cáo khoa học: Role of peroxidase inhibition by insulin in the bovine thyroid cell proliferation mechanism pdf

... mm spermidine, and maintained at 37 °C during 12 h. After inactivating the enzyme at 95 °C, during 10 min, in the presence of 5 mmEDTA, the remnant inhibitory action was assayed on the TPO enzyme ... of the time factor in signalling speciﬁcity: application to mitogenic and metabolic signalling by the insulin and insulin-like growth factor Ireceptor tyrosine kinases. Glucose Metabolism and ... during 12 h. In all cases, the proteolytic enzymes were inactivated at 95 °C, during10 min and the TPO control assay was performed in the presence of the inactivated enzymes.Effects of DNAse and...

Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx

... of thesematerials (e.g. Ne-carboxymethyl-lysines and Ne-carboxy-ethyl-lysines and pentosidine) being known to accumu-late with age on tissue proteins, and at an increasedrate in LDL and ... (1994) Binding and modiﬁcation ofproteins by methylglyoxal under physiologicalconditions. A kinetic and mechanistic study withNalpha-acetylarginine, Nalpha-acetylcysteine, and Nalpha-acetyllysine, ... esters and modiﬁed protein from glycated low-densitylipoprotein may contribute to cellular dysfunction and the increasedatherosclerosis observed in people with diabetes, and other pathologieslinked...

Báo cáo khoa học: Cysteine residues exposed on protein surfaces are the dominant intramitochondrial thiol and may protect against oxidative damage docx

... peroxynitrite(ONOO)). These ﬁndings indicate that the cysteineresidues exposed on the surface of proteins are the dominant intracellular thiol and that they may play an important role in intracellular ... borohydride and mea-suring the released GSH using the glutathione recycling assay[11]. To measure the binding of [3H]GSH to mitochondrialmembranes, membranes (1 mgÆmL)1protein) were incubated in ... by the low molecularweight thiol glutathione and by cysteine residues in the active sites of pro-teins such as thioredoxin and peroxiredoxin. In addition, there are thiolsexposed on protein...

Báo cáo khoa học: Small-molecule modulators of zymogen activation in the ﬁbrinolytic and coagulation systems pptx

... Ponting CP (1998) Evidence that the conformation of unliganded human plasminogen ismaintained via an intramolecular interaction between the lysine-binding site of kringle 5 and the N-terminalpeptide. ... C-terminal lysines, plays arole in ﬁbrinolytic propagation by serving as more efﬁ-cient binding sites for plasminogen. The high-afﬁnitylysine-binding sites in K1 and K4 may be involved in this ... Thus, plasminogen binding isessential for the ﬁbrinolytic process. The kringleligands, however, inhibit plasminogen binding to ﬁbrin and cellular receptors and, therefore, suppress ﬁbrino-lysis....

Báo cáo khoa học: DmGEN shows a ﬂap endonuclease activity, cleaving the blocked-ﬂap structure and model replication fork docx

... of the normal replication fork differing in the gap sizes of the lagging strand. The gaps in the RF2,RF3 and RF4 are 8, 4 and 0 bp, respectively. Asshown in Fig. 7C, DmGEN cleaved the lagging ... in Fig. 2B, theseactive-site residues (asterisks 2 and 3 in Fig. 2B) arehighly conserved in the I domain of the RAD2 ⁄ XPGfamily, and are important in coordinating divalentmetal ions to interact ... representation of the conserved N and I domainsof some members of the RAD2 ⁄ XPG family. The total number of amino acids in each protein and homology (% identity) between DmGEN and other members are indicated....

Báo cáo khoa học: DYNLL/LC8: a light chain subunit of the dynein motor complex and beyond pptx

... enhances the bindingafﬁnity of the dimeric DYNLT and the resultingcomplex will be rather stable [15,29]. On the otherhand, binding of DYNLRB to the preformed com-plex does not cause further binding ... fragment containing the binding sites forLC8 and Tctex1 in complex with these two light chains[15,29], and LC8 in complex with an artiﬁcially dimer-ized binding motif of EML3 [26]. The dimerization ... interacting globular domains, LC8 binding could pry apart the domains by steric constraints and might destroy furtherinteraction sites or inhibit other activities. (C) The same destabilizing effect...

Báo cáo khoa học: "All Words Domain Adapted WSD: Finding a Middle Ground between Supervision and Unsupervision" pptx

... by intro-ducing the context words as nodes into the graph and linking them with their respectivesynsets.3. These nodes corresponding to the contextwords then inject probability mass into the synsets ... class includes words which are en-countered only in the source domain D1 and donot appear in the target domain D2. Since weare interested in adapting to the target domain and since these ... domain im-proves the performance. As the amount of in- jection increases the performance approaches the skyline, and in the case of SC→H and T→Hit even crosses the skyline performance showingthat...

Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... sku and BmIVD genesTo determine the consistency between the sku pheno-type and the BmIVD genotype, linkage analysis between the wild-type and sku strains was performed.For this, crossing of the ... strainsrevealed the presence of a single point mutation in 1337 nucleotides of BmIVD. In the sku mutant, the 1127th guanine from the start codon was substitutedby thymine and none of the other sites ... leucine degradation and the sku mutant accumulates branched-chain amino acids in haemolymph, thismutant may be useful in the investigation of unique branched-chain aminoacid catabolism in insects.AbbreviationsACAD,...

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