Báo cáo khoa học: Autophosphorylation of heme-regulated eukaryotic initiation factor 2a kinase and the role of the modification in catalysis doc

... heme-regulated eIF2a kinase or heme-regulated inhibitor; KD, C-terminal kinase domain containing amino acids 145-619; KI, kinase insert with amino acids 244-371; NTD, N-terminal domain containing ... to explore the role of autophosphorylation of the HRI protein in the intramolecular protein–protein interac- tion (between the NTD and KD) and catalysis upon hem...

Ngày tải lên: 28/03/2014, 23:20

... efficiently than the N-terminal portion of the protein. The Nop53p N-terminal region, on the other hand, is involved in RNA interaction and can partially complement the conditional strain Dnop53 ⁄ GAL::NOP53 ... 4173 Maintenance and handling of E. coli and yeast strain Escherichia coli strains DH5a and BL21(DE3) were main- tained in LB medium and manipulated accordi...

Ngày tải lên: 30/03/2014, 04:20

... numerator and the denominator of Eqn (5). Based on the SD of the numerator and the denominator the SEM of q h was computed, assuming statistical independence of the two. The time-dependent metabolic ... University of Groningen, The Netherlands Introduction Living organisms have the option to regulate their molecular activities by altering expression of the...

Ngày tải lên: 18/02/2014, 06:20

... genes was induced upon treatment with the AaGlucan as well as with laminarin and sulphated laminarin, the latter being the most efficient. Of note, as for lamina- rin, the AaGlucan did not induce the ... the results of both the laminarinase and periodate treatments indi- cate that the b-1,3-linked and the periodate-sensitive residues are essential for the chitinas...

Ngày tải lên: 23/03/2014, 11:20

... the DFG loop’, for the purpose of bind- ing and positioning metal ions. The kinase domain of known eukaryotic protein kinases (ePKs) contains sev- eral conserved subdomains in addition to the ... precluded binding of the metal ions. However, when binding of the ATP took place in solution, the process was accompanied by creation of metal-binding sites. In oth...

Ngày tải lên: 30/03/2014, 16:20

... and of the kanamycin insertion mutant nirF strain (SBN03) in the upper right panel. Growth of SBN11 (DnirF) is shown in the bottom left panel and of SBN13 (DnirF) containing pEG276-nirF in the ... activity of NirF Interestingly, like NirN, NirF shares sequence similar- ity with the C-terminal d 1 heme-containing domain of cytochrome cd 1 . Strikingly, the axial...

Ngày tải lên: 15/02/2014, 01:20

... HSF1, binds to the region between )206 and )187 bp of the hsp70 promoter Next, we investigated protein binding to the region between )206 and )187 bp of the hsp70 promoter, using a biotin-mediated ... that bind to the HSEs in the promoter regions of hsp genes [19]. In addition, an in ammatory cytokine, interleukin-6, induces the exp- ression of Hsp70 and Hsp90...

Ngày tải lên: 18/02/2014, 06:20

... Seurin D, Segovia-Quinson B, Hardouin S & Binoux M (1986) Analysis of serum insulin-like growth factor binding proteins using western blotting: use of the method for titration of the binding ... binding must only be a local effect because binding of PMP-BSA, which binds to other sites in the ectodomain of these heterodimers, resulted in the predicted amount of li...

Ngày tải lên: 18/02/2014, 08:20

... reductase domain is located at the N-terminus and the predicted deaminase at the C-terminus of the bifunctional protein. In plants, orthologues of pyrimidine reductases of the riboflavin pathway ... units of guanylate kinase and 2 units of pyruvate kinase in a total volume of 0.5 mL, and were incubated for 30 min at 37 °C in order to generate the reducta...

Ngày tải lên: 18/02/2014, 18:20

... radiation response of tumor cells. However, the basis of the link between the signaling pathway and the apoptotic cell- death machinery in response to ionizing radiation remains largely unclear. The aim of our ... critically involved in the apoptotic process, and is induced by microtubule-damaging agents such as paclitaxel, docet- axel, vincristine and vinblastine...

Ngày tải lên: 07/03/2014, 05:20