Báo cáo khoa học: The predominant protein arginine methyltransferase PRMT1 is critical for zebrafish convergence and extension during gastrulation pdf

... 905–917 ª 2011 The Authors Journal compilation ª 2011 FEBS 917 The predominant protein arginine methyltransferase PRMT1 is critical for zebrafish convergence and extension during gastrulation Yun-Jung ... MO1 and MO2 indeed blocked the expression of PRMT1 protein in zebrafish embryos, both effectively and persistently. Because PRMT1 is the predomin...

Ngày tải lên: 28/03/2014, 23:20

... question whether the Tat translocon is especially ‘permissive’ towards the Rieske protein, allowing for variation at the first arginine position, or whether the stricter role of the second arginine is ... calculated for the ISP sequences and the comparison group (Fig. 2). Both curves display a positive score in the h-region which corresponds to relative hydrophob...

Ngày tải lên: 07/03/2014, 11:20

... HIS-PTI1-4 for the indicated time points. The mixes were then incubated with [c- 32 P]-ATP and MBP (10 l g) for 30 min. The top panel shows the kinase assay visualized by autoradiography and the bottom ... One tenth of the input was loaded on to the gel and represents the amount of HIS-tagged proteins used for the assay. The in vitro binding assays were repeate...

Ngày tải lên: 14/02/2014, 19:20

... Spain Introduction During HIV-1 morphogenesis [1,2], the capsid protein (CA; or p24) participates in two distinct assembly events. The first occurs inside the cell and involves the Gag polyprotein, of ... membrane, and the remaining domains forming the innermost protein layers accor- ding to their positions in Gag: MA, CA-NTD, CA-CTD, the spacer peptide SP1, NC, the s...

Ngày tải lên: 18/02/2014, 06:20

... highest affinity for the whole CA has the sequence IPVGEIYKRW, which corresponds to the a-helix 7 of NTD. This region is important for the formation of CA hexamers [6]; there- fore, this peptide could ... architecture is the production of Gag and Gag- Pol fusion proteins. The GagPol fusion protein, formed by a ribosomal frame shift event, is cleaved into the Gag and...

Ngày tải lên: 18/02/2014, 06:20

... 2 for the other tested alcohols. Statistical quality Although the focus of the mathematical approach is on the ‘leftward shift’, illustrated in Figs 1 and 2, a statisti- cal analysis was performed ... multiple-site kinetic formalism for lipid-dependent proteins has therefore previously been applied to the nAChR [23] and is now applied to tadpole narcosis. General anaes-...

Ngày tải lên: 19/02/2014, 18:20

... of KdpD in the absence and in the presence of CCCP. These results demonstrate that the pmf is required for efficient membrane insertion of KdpD with the tags. This is in agreement with the wild-type ... stimulate the transloca- tion ATPase of SecA [6,38]. The result obtained here that KdpD is independent of SecA is therefore consistent with previous findings. The res...

Ngày tải lên: 20/02/2014, 11:20

... proteomics of the capsid protein, its influence on the packaging of nonviral molecules into HIV-1 virions and the subsequent role of the molecules themselves. These inter- actions and their characterization ... cap the top and bottom of the barrel (Fig. 3) [36,37]. The active site, in a hydrophobic pocket on the protein sur- face, is the binding site of CsA and i...

Ngày tải lên: 07/03/2014, 00:20

... treated with 0, 20, 40, 60, 80 or 100 lgÆmL )1 LPS for 24 h and then the RNA and protein were isolated for RT- PCR and western blot analysis. The results demon- strated that LPS induced HBD-2 ... target the HBD-2 pro- moter. Co-IP and ChIP experiments demonstrate that HMGN2 does not interact with NF-jB directly and the two proteins do not form a complex in the nucleu...

Ngày tải lên: 22/03/2014, 16:20

... Germany) to diminish the mutation rate inherent to the PCR reaction. The cycling conditions were 94 °Cfor3min,94°Cfor40s,55°Cfor 40 s, 68 °C for 1 min per kb for 35 cycles, and 68 °Cfor 8 min. After ... evolutionary history of the astacin protein family in the nematodes. The identical and complex arrangement of the seven regulatory domains in NAS-39 and BMP-1 suggest...

Ngày tải lên: 23/03/2014, 15:21