Báo cáo khoa học: Structure, signaling mechanism and regulation of the natriuretic peptide receptor guanylate cyclase pptx

... (2011) 1818–1829 ª 2011 The Authors Journal compilation ª 2011 FEBS 1829 MINIREVIEW Structure, signaling mechanism and regulation of the natriuretic peptide receptor guanylate cyclase Kunio S. Misono 1 , ... segment, guanylyl cyclase (GC)-linked receptor that occurs as a homodimer. Here, we present an overview of the structure, possible chloride-mediated re...

Ngày tải lên: 28/03/2014, 23:20

... sequences of the cDNA and the 5¢ upstream region of zebrafish fabp3 gene. (A) The 5¢ upstream region of zebrafish fabp3 gene. The coding sequence of the first exon is shown in uppercase letters and underlined ... cDNA cloning. Both the major band of  650 bp and the minor band of  570 bp were excised, cloned and sequenced. M: 100 bp DNA ladder (with molecular s...

Ngày tải lên: 31/03/2014, 07:20

... with a change in the hydrophobicity of the local environment of Tyr63 and/ or a contribution of the binding of the copper to the thiolates of the protein. A plot of the intensity of the signal at ... min of the addition of the metal. The data corresponding to the titration of EhCopZ with cadmium were fitted using either the binding isotherm or a S...

Ngày tải lên: 23/03/2014, 12:20

... the formyl group of the c ofactor and the amino group of a protein residue. In contrast, the absence of absorption maximum at 420 nm of the mutant enzyme spectrum directly demonstrates that the ... determined by measuring the apparent K m value of D -ornithine at 25 , 50, 100, 200 and 400 l M of the inhibitor. For the measurement of the activity of the...

Ngày tải lên: 30/03/2014, 15:20

... Thermal unfolding of the protein was monitored at 203 nm at a heating rate of 60 °CÆh )1 . Effect of the recombinant protein on the activity of proteases The activity of the proteases on synthetic ... (JASCO). Fig. 3. Alignment of the sequences of the second Kunitz-modules of the human and fugu WFIKKN proteins (wfikkn_hu_2; wfikkn_fugu_2) with the second Kuni...

Ngày tải lên: 31/03/2014, 01:20

... peptide SD and the mAb, and discuss the interaction between the GA-mimic peptide and the mAb. Results 1D 1 H-NMR spectra of two synthetic cyclized decapeptides 1D 1 H-NMR spectra of the two peptides ACLPW SDGPC ... spectrum of the peptide GT are more complicated for resonance assignment than those in the 1D proton spectrum of peptide SD, a result of the...

Ngày tải lên: 16/03/2014, 23:20

... previous observations for the human and rat I-FABP, and other members of the FABP family, that the N-terminal halves of these proteins are more highly conserved than their C-terminal halves [27]. Mapping of the initiation ... m M MgCl 2 ,0.25m M of each dNTP and 0.25 l M of each inner primer. The thermal cycle conditions were the same as the primary PCR except th...

Ngày tải lên: 31/03/2014, 07:20

... entry of fatty acid into mitochon- dria. Thus, the steady-state level of malonyl-CoA in these tissues is determined by the relative activities of ACC and malonyl-CoA decarboxylase. Role of the ... ghrelin and adiponectin alter hypothalamic AMPK and malo- nyl-CoA levels [13,23,28,30–33]. The genetic evidence for the role of AMPK in the hypothalamus is less clear bec...

Ngày tải lên: 14/02/2014, 22:20

... and BHAb. The temperature dependence of the activities of the BHs was tested in the range 5–56 °C; the apparent optimum temperatures of BHLmL2 were 25–30 °C Table 3. Masses and scores of tryptic peptides ... 97% inhibition of BHLmL2 and BHAb, respectively. The influence of pH on the activities of the BH enzymes was investigated in the pH range 6–11. The...

Ngày tải lên: 18/02/2014, 14:20

... of the CA D. melanogaster sequences and, on the other hand, the other D. melanogaster sequences more closely related to the CAVI vertebrate isoforms (CAa, CAb and D. melano- gaster-3) or to the ... amplifications of RpCAbr and RpCAtr with 18S amplification. The number of cycles on the y-axis is the difference between the number of cycles required to amplify each t...

Ngày tải lên: 18/02/2014, 16:20