Báo cáo khoa học: Increased ﬂexibility and liposome-binding

Báo cáo khoa học: Increased ﬂexibility and liposome-binding capacity of CD1e at endosomal pH ppt

... circular dichroism spectra of CD1e2 ) (A) and CD1e2 + (B) were recorded at pH 7 and pH 4.8. (C) Comparison of the near-UV circular dichroism spectra of rsCD1e2 ) and rsCD1e4 ) at pH 7. MRW, mean residue ... appropri- ate buffer containing 0.15 m sodium sulfate and the indi- cated concentration of guanidium chloride. Sodium phosphate ⁄ citrate buffers (5 mm) and...

Ngày tải lên : 28/03/2014, 22:21

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Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf

... Bar graph of R 1 , R 2 and { 1 H}– 15 N NOE val- ues along the sequence of the TTF1 HD at 11.7 T and 286 K. Fig. S2. Plot of J(0.87x N )–J(0) correlation from 15 N relaxation measurements of the ... from the NOE data, have little effect on the value of the Æs mi æ estimate, and adds conﬁdence to the assumption of isotropic motion adopted by the equation of the relax- ati...

Ngày tải lên : 18/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... of Pro, Val or Lys, c-carboxylation of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues. ... region of the NOESY spectra for conomarphin and l-Phe13- conomarphin are represented in supplementary Fig. S6A,B, respectively. The NOESY data acquired at 300 K and pH 3 for...

Ngày tải lên : 18/02/2014, 17:20

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Tài liệu Báo cáo khoa học: The antibacterial and antifungal properties of trappin-2 (pre-elaﬁn) do not depend on its protease inhibitory function pptx

... volume of 90 lL of phosphate buffer, added to 100 lL of phosphate buffer containing 5 · 10 3 mid-log growth phase bacteriaÆmL )1 and the mixture was incubated for 3 h at 37 °C. The number of CFU ... mL of phosphate buffer, centrifuged again and suspended in 10 mL of phosphate buffer. Serial dilutions of this suspen- sion were plated out on Sabouraud Gentamicin Chloram- phe...

Ngày tải lên : 18/02/2014, 17:20

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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

... concentration and pH measured at 65 °C) were as follows: Na-acetate (pH 5.0, 5.4 and 5.8), Tris ⁄ HCl (pH 6.5, 7.0, 7.5 and 8.0) and glycine ⁄ NaOH (pH 8.6, 9.0 and 9.6). (B) Dependence of Rep245 ... Schematic representation and production of truncated vari- ants of the replication protein (RepA) of the plasmid pIT3 from Sulf- olobus solfataricus, strain IT3. (A) Re...

Ngày tải lên : 18/02/2014, 18:20

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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx

... leads for treating disorders associated with increased levels of quinolinate and ⁄ or picolinate. Experimental procedures PCR ampliﬁcation and cloning of ACMSD isoforms Human brain, liver and kidney ... factor 4alpha and peroxisome proliferator-activated receptor alpha. Mol Pharmacol 70, 1281–1290. 31 Colabroy KL & Begley TP (2005) Tryptophan catabolism: identiﬁcation and...

Ngày tải lên : 19/02/2014, 02:20

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Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx

... investigated the catalytic properties of HpSDH and the effects of pH and temperature on HpSDH. The results show that HpSDH has a k cat of 7.7 ± 0.9 s )1 , K m of 0.148 ± 0.028 mm and k cat ⁄ K m of 5.2 ... expressed, and puriﬁed in Escherichia coli system. The enzymatic characterization of HpSDH demonstrates its activity with k cat of 7.7 s )1 and K m of 0.148 mm...

Ngày tải lên : 19/02/2014, 05:20

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Tài liệu Báo cáo khoa học: Receptor association and tyrosine phosphorylation of S6 kinases pdf

... Thr phosphorylations and is mainly mediated via phosphatidylinositol 3-kinase (PI3K)-3-phosphoinositide-dependent protein kinase-1 (PDK1) and mTor-dependent pathways. Upstream regulators of S6K, ... (1997) Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Balpha. Curr Biol 7, 261–269. 31 Anderson KE, Coadwell J, Steph...

Ngày tải lên : 19/02/2014, 07:20

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Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt

... cells were grown and activated with 0.2 l M of phorbol 12-myristate-13-acetate (PMA) (Sigma) in 75 cm 2 tissue culture ﬂasks at 37 °C in a saturating humidiﬁed atmosphere of 95% air and 5% CO 2 . ... between NH of Thr5 and CO of Arg2, as well as NH of Arg2 and CO of Thr5. Structure of cyclic hexapeptides – cYT peptide. The NMR data of the cYT peptide wer e indicative...

Ngày tải lên : 19/02/2014, 13:20

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Tài liệu Báo cáo khoa học: The structure and biological characteristics of the Spirochaeta aurantia outer membrane glycolipid LGLB pdf

... chromatography. Gel chromatography was carried out on Sephadex G-50 (2.5 · 95 cm) and Sephadex G -15 columns (1.6 · 80 cm) in pyridinium-acetate buffer, pH 4.5 (4 mL of pyridine and 10 mL of AcOH ... USA) and lyophilization. The overall yield was determined by comparing the mass of a white powdery material left after dialysis and lyophilization ( 547 mg), to the original d ry...

Ngày tải lên : 19/02/2014, 16:20

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