Báo cáo khoa học: Increased flexibility and liposome-binding capacity of CD1e at endosomal pH ppt

... circular dichroism spectra of CD1e2 ) (A) and CD1e2 + (B) were recorded at pH 7 and pH 4.8. (C) Comparison of the near-UV circular dichroism spectra of rsCD1e2 ) and rsCD1e4 ) at pH 7. MRW, mean residue ... appropri- ate buffer containing 0.15 m sodium sulfate and the indi- cated concentration of guanidium chloride. Sodium phosphate ⁄ citrate buffers (5 mm) and...

Ngày tải lên: 28/03/2014, 22:21

... Bar graph of R 1 , R 2 and { 1 H}– 15 N NOE val- ues along the sequence of the TTF1 HD at 11.7 T and 286 K. Fig. S2. Plot of J(0.87x N )–J(0) correlation from 15 N relaxation measurements of the ... from the NOE data, have little effect on the value of the Æs mi æ estimate, and adds confidence to the assumption of isotropic motion adopted by the equation of the relax- ati...

Ngày tải lên: 18/02/2014, 16:20

... of Pro, Val or Lys, c-carboxylation of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues. ... region of the NOESY spectra for conomarphin and l-Phe13- conomarphin are represented in supplementary Fig. S6A,B, respectively. The NOESY data acquired at 300 K and pH 3 for...

Ngày tải lên: 18/02/2014, 17:20

... volume of 90 lL of phosphate buffer, added to 100 lL of phosphate buffer containing 5 · 10 3 mid-log growth phase bacteriaÆmL )1 and the mixture was incubated for 3 h at 37 °C. The number of CFU ... mL of phosphate buffer, centrifuged again and suspended in 10 mL of phosphate buffer. Serial dilutions of this suspen- sion were plated out on Sabouraud Gentamicin Chloram- phe...

Ngày tải lên: 18/02/2014, 17:20

... concentration and pH measured at 65 °C) were as follows: Na-acetate (pH 5.0, 5.4 and 5.8), Tris ⁄ HCl (pH 6.5, 7.0, 7.5 and 8.0) and glycine ⁄ NaOH (pH 8.6, 9.0 and 9.6). (B) Dependence of Rep245 ... Schematic representation and production of truncated vari- ants of the replication protein (RepA) of the plasmid pIT3 from Sulf- olobus solfataricus, strain IT3. (A) Re...

Ngày tải lên: 18/02/2014, 18:20

... leads for treating disorders associated with increased levels of quinolinate and ⁄ or picolinate. Experimental procedures PCR amplification and cloning of ACMSD isoforms Human brain, liver and kidney ... factor 4alpha and peroxisome proliferator-activated receptor alpha. Mol Pharmacol 70, 1281–1290. 31 Colabroy KL & Begley TP (2005) Tryptophan catabo- lism: identification and...

Ngày tải lên: 19/02/2014, 02:20

... investigated the catalytic proper- ties of HpSDH and the effects of pH and temperature on HpSDH. The results show that HpSDH has a k cat of 7.7 ± 0.9 s )1 , K m of 0.148 ± 0.028 mm and k cat ⁄ K m of 5.2 ... expressed, and purified in Escherichia coli system. The enzymatic characterization of HpSDH demonstrates its activity with k cat of 7.7 s )1 and K m of 0.148 mm...

Ngày tải lên: 19/02/2014, 05:20

... Thr phosphorylations and is mainly mediated via phosphatidylinositol 3-kinase (PI3K)-3-phosphoinositide-dependent protein kinase-1 (PDK1) and mTor-dependent pathways. Upstream regulators of S6K, ... (1997) Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Balpha. Curr Biol 7, 261–269. 31 Anderson KE, Coadwell J, Steph...

Ngày tải lên: 19/02/2014, 07:20

... cells were grown and activated with 0.2 l M of phorbol 12-myristate-13-acetate (PMA) (Sigma) in 75 cm 2 tissue culture flasks at 37 °C in a saturating humidified atmosphere of 95% air and 5% CO 2 . ... between NH of Thr5 and CO of Arg2, as well as NH of Arg2 and CO of Thr5. Structure of cyclic hexapeptides – cYT peptide. The NMR data of the cYT peptide wer e indicative...

Ngày tải lên: 19/02/2014, 13:20

... chromatography. Gel chromatography was carried out on Sephadex G-50 (2.5 · 95 cm) and Sephadex G -15 columns (1.6 · 80 cm) in pyridinium-acetate buffer, pH 4.5 (4 mL of pyridine and 10 mL of AcOH ... USA) and lyophilization. The overall yield was determined by comparing the mass of a white powdery material left after dialysis and lyophilization ( 547 mg), to the original d ry...

Ngày tải lên: 19/02/2014, 16:20