Báo cáo khoa học: Crystal structures of open and closed forms of D-serine deaminase from Salmonella typhimurium – implications on substrate specificity and catalysis pptx

... al. Crystal structure of D-serine deaminase FEBS Journal 278 (2011) 287 9–2 891 ª 2011 The Authors Journal compilation ª 2011 FEBS 2891 Crystal structures of open and closed forms of D-serine deaminase ... D-serine deaminase from Salmonella typhimurium – implications on substrate specificity and catalysis Sakshibeedu Rajegowda Bharath 1 ,...

Ngày tải lên: 28/03/2014, 22:20

... 21.7 – – – – 336 75.6 – – – – 337 61.7 – – – – 338 3.4 Table 2. Angles C4–O4¢–C1¢ produced by two glucose residues in the CD molecules. –, not applicable. Numbers of the two glucose residues 1–2 ... P2 1 2 1 2 1 (TvuCMBP–G4) and C2 (TvuCMBP–a-CD and TvuCMBP–b-CD) crystals were grown at 20 °C under almost the same buffer conditions. Although the...

Ngày tải lên: 29/03/2014, 23:20

... procedures Enzyme production and crystallization Gene cloning and the production, purification and crystalli- zation of TtAKb-Thr were performed as previously described [31]. TtAKb-free was crystallized by ... electron densities of the N-termi- nal (residues 1–3 in chain A, residues 1–4 in chains B and C, and residues 1–5 in chains E–G) and C-termi- nal (residues 15 8–1...

Ngày tải lên: 18/02/2014, 08:20

... key features of PDH specificity and mode of inhibition of the enzyme. Results and Discussion Structural analysis and model quality Crystal structures of three different complexes of LlPDH have ... compilation ª 2006 FEBS Crystal structures of a bacterial 6-phosphogluconate dehydrogenase reveal aspects of specificity, mechanism and mode of inhibition by analogu...

Ngày tải lên: 19/02/2014, 05:20

... network of loops connecting the strands is complicated; six loops con- nect b-strands from both b-sheets ( 1–1 ¢ 1 –2 2–2 ¢ 5 –3 5–6 ¢ 6 –6 ) and five loops connect b-strands within the same b-sheet (2 –3 ¢ ... of reaction leading to retention of configura- tion on the anomeric carbon of the b-(2,1)- D-fructofuranose. Fig. 5. Dimer of b-fructofuranosidase from...

Ngày tải lên: 06/03/2014, 00:21

... the HR2 regions). Results and Discussion Structure determination The HR1 and HR2 regions of the NiV and HeV F proteins consist of residues 13 7–1 78 and residues 45 3–4 85, respectively, and were predicted ... The A B C Fig. 1. Construction and sequence alignment of the Nipah virus (NiV) and Hendra virus (HeV) fusion cores. (A) Prediction of the heptad repeat (HR) re...

Ngày tải lên: 08/03/2014, 08:20

... Photographs and electron density maps of the SUMO-2 crystals. Shown in (A) and (B) are two different crystal forms I and II obtaine d under slightly different conditions. The sizes of crystals are ... respectively, corresponding to about o ne-quarter and more than one- third of the contact surfaces. T he interactions include two hydrogen bonds between backbone atoms of Gly27...

Ngày tải lên: 16/03/2014, 18:20

... with the conformation of active-site residues. However, mutations of Arg8 and Glu21 are rarely observed in resistant isolates [2]. One of the conformations of the side chain of Lys45 in PR forms ... chain of Met90¢ has two conformations, and one conformation has a short separation from the carbonyl oxy- gen of the catalytic Asp25¢.(B)ComparisonofMet90¢ in PR L90M and L...

Ngày tải lên: 23/03/2014, 12:20

... 1). Results Binding of AMA and odorants in solution The dissociation c onstants of the AMA–OBP c omplexes fo r the bovine and porcine OBP isoforms were, respectively, 1 .0 and 1.5 l M and the corresponding ... K diss for AMA and the concentration of AMA. Crystallization, data collection and refinement of bovine OBP Except for the bOBP–UND complex, bOBP crystals were obtaine...

Ngày tải lên: 30/03/2014, 15:20

... mgÆmL )1 of protein, 30% (w ⁄ v) PEG 1000, and 0.1 m of Tris ⁄ HCl at pH 8.5. Refinement of preliminary crystalliza- tion conditions resulted in the growth of quality crystals in a solution containing ... volume of reservoir solu- tion at 500 lL and the drop volume at 5 lL, containing 2.5 lL of protein sample and 2.5 lL of reservoir solution. AeKAT crystals were obtained in...

Ngày tải lên: 30/03/2014, 16:20