... notinteract with R124 in the ternary complex [20]. Hence, themechanism of binding of fosfomycin by reinforcement of aninitial binding site (mainly K22 and R397) through recruit-ment of secondary ... charged amino acid sidechains, R397 and R120, are also involved in fosfomycinbinding, and in view of the multitude of interactions, it wasassumed that deletion of a single interaction would not ... chain of K22 participates in fosfomycin binding, thus providing a rationale for the loss of fosfomycin binding to the K22V and K22E mutant proteins.However, two other positively charged amino...