... binding event was greater than 30 l
M
.In
this case the exact K
d
value was not assessed by Scatchard
analysis because the highest SOCS-3 concentration was
30 l
M
and a calculation by the
BIAEVALUATION
software
Fig. ... 5¢-CGGGCTCGGC
ACTGAGCAGCGCGTTCGCCTCGCCGCCGGTC-3¢
(L5 8A as), 85¢-CAGTCTGGGACCAAGAACGCGCGC
ATCCAGTGTGAGGGG-3¢ (L9 3A s), 5¢-CCCCTCACA
CTGGATGCGCGCGTTCTTGGTCCCAGACTG...
... Surprisingly, the microsomal fraction of an
extract of the resistant insect D. melanogaster also displayed
a strong binding capacity.
The determination of the characteristics of the binding
site of these ... characterized the binding of PA1b to a
proteinaceous component of a particulate fraction of
S. oryzae extracts. The binding was saturable and...
... construct
containing Y6 41 alone was not phosphorylated by any of the
kinases, confirming that Y6 41 is probably not a site for
phosphorylation (data not shown).
SHP-1 and SHP-2 association with the cytoplasmic ... kinases. By
comparison, the mutation of the tyrosine at position 641 did
not significantly affect the degree of phosphorylation by any
of the kinases that...
... comprising the sidechain of the active site base
and the sidechain of the catalytically active modified
tryptophan of the TTQ that forms an adduct with
methylamine (Fig. 2B). QM/MM calculations were then
performed ... between enzyme dynamics and
tunneling [29] and in at least one case tunneling has been
invoked in the reappraisal of the catalytic mechanism of the
as...
... [18].
For the enzymatic equivalent of this reaction the actions
of a hydratase and an aldolase are needed. Citral lyase
of P. digitatum combines hydratase and aldolase activity
in a single enzyme. ... been
reports on enzymes combining the action of a hydratase
with that of an aldolase. The best studied is enoyl-CoA
hydratase/aldolase [26] (also known as 4-hydrox-
y...
... absorbance values at 405 nm. The (apparent)
K
d
values for aegyptin–collagen interaction were calculated
by non-linear regression analysis of the binding data with
graphpad prism software (GraphPad Software, ... that mediates collagen interaction with von Willebrand fac-
tor (vWF) as a high- affinity binding site for aegyptin, with a K
D
of approxi-
mately 5 nm. Additi...
... [35].
Secondly, we employed a cytotoxicity assay, since the ADP-
ribosylation activity of ExoS mediates a marked change in
cell morphology and has a lethal activity upon translocation
into the host ... modification assay, where ADP-ribosylation of
Ras by ExoS is reflected by a gel mobility shift of Ras on
SDS/PAGE [35]. Incubation of Ha-Ras, 14-3-3, NAD and
GST alone does...
... globule state as the
latter is almost as compact as the folded state ( radius of
gyration and hydrodynamic radius » 10±30% larger), has a
hydrophobic core and contains a large amount of secondary
structure ... a
S1
-and
a
S2
-casein, these proteins had a tendency to aggregate in the
laser beam, which prevented the acquisition of ROA data of
suf®cient quality for re...
... initiation codons for panD and
aq477, they appear to be organized as an operon.
panD encodes an aspartate decarboxylase which cata-
lyses the decarboxilation of aspartate to produce
b-alanine, a precursor ... Tanaka Y, Tsumoto K, Yasutake Y, Umetsu M, Yao
M, Fukada H, Tanaka I & Kumagai I (2004) How
oligomerization contributes to the thermostability of an
archaeon protein. P...
... trine.staff@snla.no
1
Department of Research, Norwegian Air Ambulance Foundation, Drøbak,
Norway
Full list of author information is available at the end of the article
Staff and Søvik Scandinavian Journal of Trauma, ... light-and-siren response for the study purpose
only.
This study of documentation quality was part of a
cross-sectional MVA study evaluating whether patie...