Báo cáo Y học: A new high affinity binding site for suppressor of cytokine signaling-3 on the erythropoietin receptor potx

Báo cáo Y học: A new high affinity binding site for suppressor of cytokine signaling-3 on the erythropoietin receptor potx

Báo cáo Y học: A new high affinity binding site for suppressor of cytokine signaling-3 on the erythropoietin receptor potx

... binding event was greater than 30 l M .In this case the exact K d value was not assessed by Scatchard analysis because the highest SOCS-3 concentration was 30 l M and a calculation by the BIAEVALUATION software Fig. ... 5¢-CGGGCTCGGC ACTGAGCAGCGCGTTCGCCTCGCCGCCGGTC-3¢ (L5 8A as), 85¢-CAGTCTGGGACCAAGAACGCGCGC ATCCAGTGTGAGGGG-3¢ (L9 3A s), 5¢-CCCCTCACA CTGGATGCGCGCGTTCTTGGTCCCAGACTG...
Ngày tải lên : 24/03/2014, 00:21
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Báo cáo khoa học: Characterization of a high-affinity binding site for the pea albumin 1b entomotoxin in the weevil Sitophilus ppt

Báo cáo khoa học: Characterization of a high-affinity binding site for the pea albumin 1b entomotoxin in the weevil Sitophilus ppt

... Surprisingly, the microsomal fraction of an extract of the resistant insect D. melanogaster also displayed a strong binding capacity. The determination of the characteristics of the binding site of these ... characterized the binding of PA1b to a proteinaceous component of a particulate fraction of S. oryzae extracts. The binding was saturable and...
Ngày tải lên : 08/03/2014, 02:21
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Báo cáo Y học: A new siglec family member, siglec-10, is expressed in cells of the immune system and has signaling properties similar to CD33 docx

Báo cáo Y học: A new siglec family member, siglec-10, is expressed in cells of the immune system and has signaling properties similar to CD33 docx

... construct containing Y6 41 alone was not phosphorylated by any of the kinases, confirming that Y6 41 is probably not a site for phosphorylation (data not shown). SHP-1 and SHP-2 association with the cytoplasmic ... kinases. By comparison, the mutation of the tyrosine at position 641 did not significantly affect the degree of phosphorylation by any of the kinases that...
Ngày tải lên : 24/03/2014, 04:21
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Báo cáo Y học: A new conceptual framework for enzyme catalysis Hydrogen tunneling coupled to enzyme dynamics in flavoprotein and quinoprotein enzymes docx

Báo cáo Y học: A new conceptual framework for enzyme catalysis Hydrogen tunneling coupled to enzyme dynamics in flavoprotein and quinoprotein enzymes docx

... comprising the sidechain of the active site base and the sidechain of the catalytically active modified tryptophan of the TTQ that forms an adduct with methylamine (Fig. 2B). QM/MM calculations were then performed ... between enzyme dynamics and tunneling [29] and in at least one case tunneling has been invoked in the reappraisal of the catalytic mechanism of the as...
Ngày tải lên : 31/03/2014, 23:20
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Tài liệu Báo cáo Y học: A novel, inducible, citral lyase purified from spores of Penicillium digitatum docx

Tài liệu Báo cáo Y học: A novel, inducible, citral lyase purified from spores of Penicillium digitatum docx

... [18]. For the enzymatic equivalent of this reaction the actions of a hydratase and an aldolase are needed. Citral lyase of P. digitatum combines hydratase and aldolase activity in a single enzyme. ... been reports on enzymes combining the action of a hydratase with that of an aldolase. The best studied is enoyl-CoA hydratase/aldolase [26] (also known as 4-hydrox- y...
Ngày tải lên : 21/02/2014, 01:21
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Báo cáo khoa học: Aegyptin displays high-affinity for the von Willebrand factor binding site (RGQOGVMGF) in collagen and inhibits carotid thrombus formation in vivo ppt

Báo cáo khoa học: Aegyptin displays high-affinity for the von Willebrand factor binding site (RGQOGVMGF) in collagen and inhibits carotid thrombus formation in vivo ppt

... absorbance values at 405 nm. The (apparent) K d values for aegyptin–collagen interaction were calculated by non-linear regression analysis of the binding data with graphpad prism software (GraphPad Software, ... that mediates collagen interaction with von Willebrand fac- tor (vWF) as a high- affinity binding site for aegyptin, with a K D of approxi- mately 5 nm. Additi...
Ngày tải lên : 22/03/2014, 21:20
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Báo cáo Y học: A nonphosphorylated 14-3-3 binding motif on exoenzyme S that is functional in vivo pot

Báo cáo Y học: A nonphosphorylated 14-3-3 binding motif on exoenzyme S that is functional in vivo pot

... [35]. Secondly, we employed a cytotoxicity assay, since the ADP- ribosylation activity of ExoS mediates a marked change in cell morphology and has a lethal activity upon translocation into the host ... modification assay, where ADP-ribosylation of Ras by ExoS is reflected by a gel mobility shift of Ras on SDS/PAGE [35]. Incubation of Ha-Ras, 14-3-3, NAD and GST alone does...
Ngày tải lên : 31/03/2014, 08:20
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Tài liệu Báo cáo Y học: A Raman optical activity study of rheomorphism in caseins, synucleins and tau New insight into the structure and behaviour of natively unfolded proteins pot

Tài liệu Báo cáo Y học: A Raman optical activity study of rheomorphism in caseins, synucleins and tau New insight into the structure and behaviour of natively unfolded proteins pot

... globule state as the latter is almost as compact as the folded state ( radius of gyration and hydrodynamic radius » 10±30% larger), has a hydrophobic core and contains a large amount of secondary structure ... a S1 -and a S2 -casein, these proteins had a tendency to aggregate in the laser beam, which prevented the acquisition of ROA data of suf®cient quality for re...
Ngày tải lên : 21/02/2014, 15:20
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Báo cáo khoa học: A new sulfurtransferase from the hyperthermophilic bacterium Aquifex aeolicus Being single is not so simple when temperature gets high potx

Báo cáo khoa học: A new sulfurtransferase from the hyperthermophilic bacterium Aquifex aeolicus Being single is not so simple when temperature gets high potx

... initiation codons for panD and aq477, they appear to be organized as an operon. panD encodes an aspartate decarboxylase which cata- lyses the decarboxilation of aspartate to produce b-alanine, a precursor ... Tanaka Y, Tsumoto K, Yasutake Y, Umetsu M, Yao M, Fukada H, Tanaka I & Kumagai I (2004) How oligomerization contributes to the thermostability of an archaeon protein. P...
Ngày tải lên : 30/03/2014, 03:20
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Báo cáo y học: " A retrospective quality assessment of pre-hospital emergency medical documentation in motor vehicle accidents in south-eastern Norway"

Báo cáo y học: " A retrospective quality assessment of pre-hospital emergency medical documentation in motor vehicle accidents in south-eastern Norway"

... trine.staff@snla.no 1 Department of Research, Norwegian Air Ambulance Foundation, Drøbak, Norway Full list of author information is available at the end of the article Staff and Søvik Scandinavian Journal of Trauma, ... light-and-siren response for the study purpose only. This study of documentation quality was part of a cross-sectional MVA study evaluating whether patie...
Ngày tải lên : 25/10/2012, 09:56
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