Molecular Biotechnology-Lession 3: Basic techniques in DNA technology ppt
Molecular Biotechnology-Lession 3: Basic techniques in DNA technology ppt
... chain reaction (PCR) mRNA DNA cDNA Reverse transcriptase Taq DNA polymerase In RT-PCR, an RNA strand is first reverse transcribed into its DNA complement (complementary DNA, or cDNA) using ... Components of RT-PCR 44 o C, 1hr 92 o C, 10min 4 o C, 0min Step 1: Making cDNA Step 2: Regular PCR Basic techniques in DNA technology Gene cloing
Ngày tải lên: 23/03/2014, 22:20
Tài liệu Use of Solar Process Heat – a Challenge in Brewing Technology pptx
... distribution in the mash • Fast mash heating • Minimal fouling of the heating surface • Processing with little oxygen uptake The target of stirring in the mash vessel is: Flow patterns in the mash ... with inclined agitator blades arranged in opposite direction • Circular movement and “folding” of the mash • Optimal mixing in the dead zone behind the blade due to holes in the b...
Ngày tải lên: 22/02/2014, 05:20
Báo cáo khoa học: "EXPERT SYSTEMS AND OTHER NEW TECHNIQUES IN MT SYSTEMS " ppt
... metho- dology by incorporating advanced techniques from software engineering, moving toward third-genera- tion systems by incorporating expert systems, and returning to interactive techniques for ... that main- taining and upgrading the lingware, even in an admittedly limited second generation CAT system, requires a good deal of expertise. Techniques are now being developed to...
Ngày tải lên: 08/03/2014, 18:20
West Bengal University of Technology BF-142, Salt Lake City, Kolkata-700064 Structure & Detailed Syllabus of B.Tech in Food Technology ppt
... (using C/C++ languages) Stacks and Queues : Adding, Deleting elements, Circular Queue : Adding and Deleting elements, Merging Problem. Implementation of linked lists : Inserting, Deleting, Inverting ... Principles of comminution, Types of comminuting equipment. Energy and power requirement, Crushers, Grinders, Mixing and Agitations, Power consumption in mixing, Mechanical separation, S...
Ngày tải lên: 18/03/2014, 00:20
Tài liệu Environmental Technology Series - Part 1: Rapid Inventory Techniques in Enviromental Polllution doc
Ngày tải lên: 12/02/2014, 15:20
Báo cáo khoa học: Okadaic acid induces DNA fragmentation via caspase-3-dependent and caspase-3-independent pathways in Chinese hamster ovary (CHO)-K1 cells pot
... knockout or inhibition of caspase-3 activity is ineffective in preventing OA-induced DNA fragmentation in CHO-K1 cells, z-DEVD-fmk was successful at inhibiting OA-induced DNA fragmentation in caspase-3 ) ... and also inhibited OA-induced DNA frag- mentation (Fig. 3A), suggesting that AEBSF-sensitive, but TPCK-insensitive or TLCK-insensitive serine pro- teases, are involved in OA...
Ngày tải lên: 22/03/2014, 21:20