Báo cáo khoa học: Altering the surface properties of baculovirus Autographa californica NPV by insertional mutagenesis of the envelope protein gp64 ppt

... the baculovirus envelope (Eur. J. Biochem. 269) 4467 Altering the surface properties of baculovirus Autographa californica NPV by insertional mutagenesis of the envelope protein gp64 Alexandra ... the native envelope protein gp64. Thereby, no duplication of the gp64 is necessary to target the foreign peptide to the surface of baculo...

Ngày tải lên: 23/03/2014, 21:21

... modified by mutation [14] or Ca-depletion [13] significant chan- ges in the properties of the ferric form of the protein has been detected. Even so, the changes detected in the haem cavity of the reduced ... in the disorder and hence entropy of the reduced state of the mutant compared to that of the wild-type protein. In contrast to the present study, i...

Ngày tải lên: 07/03/2014, 21:20

... focusing of proteins. Methods Enzymol 22, 389–412. 49 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein- dye ... change in the amount of GST protein present, a covalent modifi- cation of GST that alters its properties, or a change in the mixture of GST isozymes present in the...

Ngày tải lên: 23/03/2014, 15:20

... migrate through the extracellular matrix with the help of matrix metalloproteinase [82]. The consequent event of brain macrophages is one of the most important defense mechanisms used by the brain. ... 2009) doi:10.1111/j.1742-4658.2009.07174.x The vascular network of the brain is formed by the invasion of vascular sprouts from the pia mater toward the ventri...

Ngày tải lên: 18/02/2014, 11:20

... achieved. Determination of the structure of 8K-BLP MALDI-TOF MS analysis of the purified material from the final HPLC yielded a monoisotopic mass of 7409.2 ([M + H] + ) (Fig. 3A). Amino acid sequence analysis of the ... to those of bombyxin and insulin, which are of the order of 100 pm [23,24]. The very high titer of 8K-BLP may be due to a remarkably high level of...

Ngày tải lên: 18/02/2014, 13:20

... be part of the defense sys- tem against wounding and infection by the pathogen [16]. Thus, the timing and location of melanization seems to be essential for the prevalence of one of these two ... 1.10.3.1), but the differentiation between these two types of enzyme is not clear [4]. Looking at the sequences of the two enzymes, both show absolute conservation of t...

Ngày tải lên: 19/02/2014, 07:20

... classification task. 7 The nai- ve Bayes (NB) algorithm estimates the conditional probability of a set of features given a label, using the product of the probabilities of the individual features ... /'YES'@[102]. The different number of positions considered to the left and right of the markers in our training corpus, as well as the nature of t...

Ngày tải lên: 20/02/2014, 15:20

... for 10 min a t either 3 0 °Cor42°C, the t wo cultures were labelled for 1 min at the indicated temperature by the addition of 35 S[methionine] (50 mCiáL )1 ) and chased by the addition of nonradioactive ... sequence-speci®c cleavage by SPases and removal of the s ignal peptide from the precursor (export) protein [4,6±8]. Minor differences between individual signal pe...

Ngày tải lên: 21/02/2014, 03:20

... (i.e., the eigenvector correspond- ing to the largest eigenvalue) is the direction in which the sum of the square of the projections of the row vectors of the matrix is maximum. In fact, the principal ... three taken together. The individual contribution of the other eigenvalues to the spectrum is significantly lower than that of the top three. Third, the...

Ngày tải lên: 22/02/2014, 02:20

... the safety of MB, the absence of undesirable effects on the central nervous system, and the lack of induction of inflammatory responses [22]. Despite a long-standing interest in the field of mura- myl ... immunomodulators to reveal the mechanism of action of toxic MDPs on mitochondrial respiration by correlating the uncoupling effect induced by these molecules wi...

Ngày tải lên: 05/03/2014, 23:20