Báo cáo khoa học: Cellular uptake of antisense oligonucleotides after complexing or conjugation with cell-penetrating model peptides pdf

... Cellular uptake of antisense oligonucleotides after complexing or conjugation with cell-penetrating model peptides J. Oehlke, P. Birth, E. Klauschenz, ... nonendocytic mode of uptake, the limited influence of complexing or covalently tagging with cell penetrating peptides and the negligible role of structure forming properties of the peptide upon the...

Ngày tải lên: 23/03/2014, 21:21

... extracted with 1 M KCl or incubated for 1 h with one of the following components prepared in lysis buffer: 0.1 M Na 2 CO 3 pH 11; 125 mM NaCl; 1% TX-100; 1% TX-100 together with 125 m M NaCl; or sonicated ... anti- rabbit IgG (Jackson Immunoresearch Laboratories, West Grove, PA, USA) at a dilution of 1 : 200 for 45 min. Fila- mentous actin expression was detected with Alexa Fl...

Ngày tải lên: 19/02/2014, 16:20

... structure of the network in Fig. 3 correctly. For example, the estimate of the Jacobian shows a large direct effect of gene 1 on gene 4, which is incorrect. The singular vector u 1 , corresponding ... Furthermore, we have only considered the case of the estimation around a stable steady-state of the network. In the case of oscillations, created within the network or affecting th...

Ngày tải lên: 07/03/2014, 17:20

... ICDH shows a clear preference for NADP + as a cofactor although its K app m wasratherhighwith90l M . This low affinity for the cofactor NADP + was also observed for one of the human isoenzymes and ... available mixture of D , L -isocitrate was established prior performing the assay according to [30]. The specific activity of ICDH-2 at saturation of all three substrates and cofactors was...

Ngày tải lên: 08/03/2014, 02:20

... for a model of the interactions of the two T. cruzi proteins, which, together with our identification of the specificity-con- ferring interactions, will be important for development of drugs for ... reliable model of chagasin–cruzipain interactions. Such information, coupled with our identification of specifi- city-conferring interactions, should be important for the developme...

Ngày tải lên: 16/03/2014, 04:20

... effects, introduction of favorable aromatic–aromatic interactions on the surface, improved formation of secondary structures and release of conformational strain, stabilization of an intrinsic metal ... half-life of III-2 was 568 min while that of wild-type was less than 1 min at 80 °C. When the thermostability of the shuffling mutants of BTMA was compared with that of the...

Ngày tải lên: 16/03/2014, 13:20

... phase, the model transforms discourse structure (DS) information in the corpus into DSBs by comparing the DS information of an utterance with that of the previous utterance. After transformation, ... the results of the integrated model are worse than the results of each analysis model. For top-1 candidate, the performance of the speech act analysis fell off about 2.89%...

Ngày tải lên: 17/03/2014, 07:20

... bottom left part of the gel. The percentage of a given spot was estimated with the use of two different measures. The inten- sity of the spot of interest was divided by the sum of all major spots. Alternatively, ... 611–617. 22 Fiorillo F, Palocci C, Soro S & Pasqua G (2007) Latex lipase of Euphorbia characias L.: an aspecific acylhydrolase with several isoforms. Plant Sc...

Ngày tải lên: 22/03/2014, 17:20

... changes with time. For binding processes, the forward rates describe the rates of association of enzyme–ligand complexes and the reverse rates describe the rates of dissociation. For example, for the ... most important formulas to connect observed quanti- ties with model parameters are summarized below. The expression for the initial concentration of ER allows to derive analytic...

Ngày tải lên: 22/03/2014, 21:20

... absence of ionophore, 74% of AmyH is in the mature processed form after 10 min, and 85% of AmyH is mature after 30 min of chase treatment. The rate of translocation Table 1. Minimal inhibitory concentrations ... concentration of 50% of the MIC, AmyH was secreted at normal levels in the presence of all ionophores with the exception of monensin (Table 1). In particular,...

Ngày tải lên: 23/03/2014, 06:20