Báo cáo khoa học: NADH oxidation and NAD+ reduction catalysed by tightly coupled inside-out vesicles from Paracoccus denitrificans doc

... NADH oxidation and NAD + reduction catalysed by tightly coupled inside-out vesicles from Paracoccus denitrificans Alexander B. Kotlyar and Natalia Borovok Department ... Israel Tightly coupled inside-out vesicles were prepared from Paracoccus denitrificans cells (SPP, sub -Paracoccus particles) and characterized kinetically. The rate of NADH oxida...

Ngày tải lên: 23/03/2014, 21:21

... and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of 1 nm. Thermal stability of Rep245 was measured by incu- bating the protein at 60, 70 and 80 °C for 30 min and ... Na-acetate (pH 5.0, 5.4 and 5.8), Tris ⁄ HCl (pH 6.5, 7.0, 7.5 and 8.0) and glycine ⁄ NaOH (pH 8.6, 9.0 and 9.6). Reactions were carried out for 30 min at 65 °C and the produc...

Ngày tải lên: 18/02/2014, 18:20

... could be eliminated by randomized sample loading, and error reduction was achieved by the use of normalizers or calibrators in combination with computational data processing. By converting relative ... generated under standardized conditions is critical for understanding dynamic cellular processes. We report strategies for error reduction, and algorithms for automated data proces...

Ngày tải lên: 19/02/2014, 07:20

... sequencing and MALDI-TOF MS analy- sis. This work was supported partly by a Creative Scientific Research from the Japan Society for the Pro- motion of Science (JSPS-13GS0023) and partly by an Intellectual ... acetylornithine and glutamate to form acetylglutamate and ornithine, thereby functioning in the first and fifth steps in citrulline biosynthesis. CLGAT enzyme purified 7000-f...

Ngày tải lên: 20/02/2014, 03:20

... between A and B number of spurts between A and B number of spurts of speaker B between A and B do A and B overlap? Durational features: duration of A if A and B do not overlap: time separating A and B if ... “yes” and “right”, as listed in (Cohen, 2002), general cue phrases, e.g. “but” and “alright” (Hirschberg and Litman, 1994), and adjectives with positive or negative po...

Ngày tải lên: 20/02/2014, 16:20

... an important component of the ligands recognized by the lectins. Recognition can be affected by specific structural variations and modifications of sialic acids and their linkage to the underlying ... antennarius [12], Scylla serrata [24] and Penaeus monodon [27]. The lectin from Scylla serrata was NeuGc specific [24] and that from Cancer antennarius was 9-O-NeuAc and 4-O-NeuAc...

Ngày tải lên: 21/02/2014, 00:20

... This work was supported by research grants from the Spanish (BMC2001-563 and BFU2004-00843, MEC) and Andalusian Regional (PAI group CVI-261) Adminis- trations, funded in part by the EU FEDER program. References 1 ... UÆmg )1 protein and recovery levels of 20– 30%. In all cases, the analysis by SDS ⁄ PAGE and native PAGE of purified preparations showed only one protein band of 2...

Ngày tải lên: 16/03/2014, 11:20

... tetracycline and ampicillin and induced with isopropyl thio-b- D -galactoside. Bacteria were harvested and lysed by French Press. The whole cell lysate (WCL) was separated into soluble and insoluble ... (Figs 3, 4 and 6 and Table 1), and (e) the rocF gene and RocF protein shared homology with the arginase/agmatinase superfamily [8]. These results demonstrate that rocF is nec...

Ngày tải lên: 16/03/2014, 16:20

... activity was potently inhibited by Cu 2+ , Ni 2+ and Zn 2+ . This enzyme was inhibited by some agents, butanediol and p-chloromercuric sulfonic acid, which modify arginine and cysteine residues. The ... sulfate lyases and heparinase III, from B. sterocoris were not activated by salts. Therefore, to purify salt-active heparin lyase, B. stercoris HJ-15 cells were disrupted by...

Ngày tải lên: 23/03/2014, 21:20

... Fe(III) sulfate by NADH catalyzed by cytosolic and membrane fractions. The former reaction differed markedly from the latter by about 10-times lower apparent K m value for NADH, by a discernible ... enzymes (FerA and FerB) catalyzing the reduction of a number of iron(III) complexes by NADH, were purified to near homogeneity from the aerobically grown iron-limited cultu...

Ngày tải lên: 30/03/2014, 13:20