... but increases the activity of theZn2+-containing APase [3,4]. Significant cooperative inter-actions have been detected during metal-ion binding,positive for the binding of Zn2+to the M1 site, ... Mg2+concentration, is probably dueto Mg2+binding to the zinc binding sites (M2 and M1).It appears that in contrast to the binding of Zn2+to thesecond M3 site, Mg2+binding in the range of Mg2+concentrations ... P i binds toAPase with negative cooperativity [6,8,9,24,25], the thermalinactivation has biphasic kinetics [26], and curve-fittingindicates that the deviations from Michaelis–Menten kine-tics...