Báo cáo khoa học: Functional similarities between the small heat shock proteins Mycobacterium tuberculosis HSP 16 3 and human aB-crystallin docx

... on pET16b -HSP 16. 3 to ensure that the HSP 16. 3 gene was i nserted in the proper orientation, and the coding sequence of HSP 16. 3 was verified by DNA sequence analysis. The pET16b -HSP 16. 3 vector ... Functional similarities between the small heat shock proteins Mycobacterium tuberculosis HSP 16. 3 and human aB-crystallin Melissa M...

Ngày tải lên: 23/03/2014, 21:21

... horylation, and recruitment of HSP2 7 and aB-crystallin to aggresomes. J. Biochem. (Tokyo) 131 , 5 93 6 03. 25. den Engelsman, J., Keijsers, V., de Jong, W.W. & Bo elens, W.C. (20 03) The small heat- shock ... kDa, which may comprise various other small heat- shock proteins, such as aA-crystallin in the eye lens, and HSP2 7 and HSP2 0 in muscle cells [5,6]. Ph...

Ngày tải lên: 23/03/2014, 13:20

... plant sHsp, Triticum aestivum (wheat) TaHsp16.9 C-I, and an sHsp from a prokaryotic archeaon, Methanococcus jannaschii MjHsp16.5. Although TaHsp16.9C-I is a dodecameric disk [7], and MjHsp16.5 forms ... for TaHsp16.9C-I and 1 73 kDa for TaHsp17.8C-II. These data are consistent with a more extended shape for TaHsp16.9C-I than for TaHsp17.8C-II and with a dodecameric organiza- tion for...

Ngày tải lên: 07/03/2014, 15:20

... residues from H sp27 [25] and 33 residues from Hsp2 5 [33 ], although the latter modifica- tion was small. In contrast, loss of 42 residues from the N-terminus of a rice small heat shock/ a-crystallin protein increased ... transformed w ith Hsp2 5, and Hsp2 5 lacking 33 N-terminal amino-acid residues, are equally heat tolerant [33 ]. Removal of C-terminal exten- sions from...

Ngày tải lên: 22/02/2014, 04:20

... DEC1:4– 233 2-R65A) abolished DEC1 binding to the E-box (lanes 6 and 16) . On t he other hand, deletion of up to 2 73 residues from the C-terminal region of DEC1 (VP16-DEC1:4– 232 and VP16-DEC1:4– 139 ) ... DEC1:119–412) abolished the suppression (Fig. 1A). On the other h and, deletion of up to 2 73 residues from the C-terminal region of DEC1 (DEC1:1 30 9, DEC1:1– 232 and...

Ngày tải lên: 19/02/2014, 16:20

... SE WB: anti-HA 204 264 428 428 33 3 264 33 3 ∆264 -33 3 ∆264 -33 3 ∆204-264 ∆264 -33 3 FLAG-∆204-264 FLAG-RPL4 (WT) FLAG-RPL4 (∆204-264) FLAG-RPL4 (∆264 -33 3) FLAG-∆264 -33 3 HA-Guα 1 1 Fig. 6. Reversal ... M3 M4 M5 M6 264 33 3 428 131 131 131 196 264 33 3 264 204 204 264 – – + – + + Nucleoplasm and cytoplasm Nucleoli Nucleoli Nucleoplasm and cytoplasm Nucleoplasm Nucleoplasm...

Ngày tải lên: 20/02/2014, 01:20

... 21 February 20 03, revised 28 April 20 03, accepted 12 May 20 03) Eur. J. Biochem. 270, 2 837 –2847 (20 03) Ó FEBS 20 03 doi:10.1046/j.1 432 -1 033 .20 03. 036 61.x recombination or repair [16] . Ydc2-deficient ... involve the steric ÔpinÕ ) the loop protruding between helices 3 and 4 at the junction crossover – and the unpairing of four base-pairs in the centre of the j...

Ngày tải lên: 20/02/2014, 11:20

... second dimension, and then probed for Pex1p and Pex6p on the same blot. AAA complex and peroxisomal importomer K. Rosenkranz et al. 38 06 FEBS Journal 2 73 (2006) 38 04 38 15 ª 2006 The Authors Journal ... peroxisomal importomer FEBS Journal 2 73 (2006) 38 04 38 15 ª 2006 The Authors Journal compilation ª 2006 FEBS 38 15 Functional association of the AAA complex and t...

Ngày tải lên: 07/03/2014, 12:20

... transmembrane domain. (Received 30 October 2002, revised 20 January 20 03, accepted 10 February 20 03) Eur. J. Biochem. 270, 14 83 1492 (20 03) Ó FEBS 20 03 doi:10.1046/j.1 432 -1 033 .20 03. 035 14.x been demonstrated ... at the other [5], andthathydrolysisatthetwoNBDsmayoccurinan alternative fashion [6]. However, whether the two NBDs have a functionally identical role in the trans...

Ngày tải lên: 08/03/2014, 08:20

... F200K and P2. Furthermore, the F193A and W373K muta- tions do not result in any change in the thermostability of the enzyme (Table S2), and thermal unfolding of the wild-type and both the F193A and ... explain the observed changes in kinetic parame- ters of the F1 93 mutant. The large decrease in k cat for the W373K mutant was unexpected, but the findings are con...

Ngày tải lên: 16/03/2014, 04:20