Báo cáo khoa học: Dimerization of mammalian adenylate cyclases Functional, biochemical and ¯uorescence resonance energy transfer (FRET) studies pot

... PRIORITY PAPER Dimerization of mammalian adenylate cyclases Functional, biochemical and ¯uorescence resonance energy transfer (FRET) studies Chen Gu 1 , James J. Cali 2 and Dermot M. F. Cooper 1,3 1 Neuroscience ... protein molecular masses of  120 kDa, again sugge sted a higher order assembly of adenylate cyclases. Mammalian adenylate cyclases...

Ngày tải lên: 23/03/2014, 21:21

... 3D). Both phosphorylation of Sav and its increase in abundance were dependent of the ability of Sav and Mst to interact. Deletion of the coiled-coil domain, and thus the ability of the two proteins ... growth and apoptosis in Drosophila in vivo, primarily through the regulation of Yki activity. Hpo is a Drosophila orthologue of the Ste20-like kinases, and is most simil...

Ngày tải lên: 19/02/2014, 06:20

... cosedimentation assay, in the absence and in t he presence of troponins. In the absence of troponins, binding of Tms to actin was very weak and only small amounts of Tm wer e detected in gels after ... stability of human Tm CD and DSC experiments were used as methods for evaluating the effect of mutations on the stability of Tms [34]. The thermal-induced unfolding of the...

Ngày tải lên: 16/03/2014, 18:20

... existence of a large variety of target genes involved in major functions in the organism [15,82,83]. Tables 4 and 5 and Fig. 2 illustrate both the diversity of the studies into the effect of glutamine ... J & Tunon MJ (2008) Effects of glutamine on proinflammatory gene expression and activation of nuclear factor kappa B and signal transducers and activators of tra...

Ngày tải lên: 07/03/2014, 00:20

... Grolli 5 and Roderic G. Eckenhoff 1,3 1 Department of Anesthesia, University of Pennsylvania, Philadelphia, PA, USA 2 Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, ... halothane and isoflurane bound with K d values of 80 ± 10 lm and 100 ± 10 lm, respectively. Halothane and isoflurane binding resulted in an overall stabilization of the...

Ngày tải lên: 07/03/2014, 16:20

... in the upper band, but does not affect the intensity of the lower (specific) band. The Sp1 probe shows three bands, with the lowest band migrating at the position of the upper band obtained with ... lung and liver under standard conditions as described previously [17]. Three micromolar CcO was dialyzed in the presence of 0.1 mm ATP and 120 lm cardiolipin to remove cholate and to re...

Ngày tải lên: 30/03/2014, 03:20

... site with binding stoichiome- try (n) of 0.3 ± 0.2 and a binding constant (K d )of 3.4 ± 4 · 10 )9 m and a second with a stoichiometry of 0.7 ± 0.1 and a K d of 3.3 ± 3 · 10 )7 m (Fig. 5A). Identical ... 4C). The decrease in some of the free state resonances and the appearance of new resonances upon complex formation indicated a slow exchange on the NMR timescale between t...

Ngày tải lên: 16/02/2014, 14:20

... over-expres- sion of Hsp60 and Hsp70 and exhibit abnormal mor- phology in correlation with increased proliferation, premature differentiation and elongation, inhibition of denucleation and of loss of organelles ... mechanisms for the lack of HSF2. In line with this hypothesis, double disruption of Hsf1 and Hsf2 is associated with sterility and complete arrest of sper...

Ngày tải lên: 18/02/2014, 04:20

... target, loading of the appropriate guide strand into the RISC is critical. Improved understand- ing of the interactions of siRNAs with TRBP and Dicer will enable improved design of siRNA therapeutics. In ... impact of silencing TRBP and Dicer on the function of the fully paired and mismatched sequences, we wished to examine Fig. 1. Effect of guide strand 5¢ end mismatch on...

Ngày tải lên: 18/02/2014, 06:20

... concentra- tion of G418 (Invitrogen) of 400 lgÆmL )1 . G418-resistant colonies were picked and expanded for PCR genotyping and the formation of embryoid bodies. One ESC clone (T26) out of 48 G418-resistant ... roles in develop- ment and in health and disease [1]. Their properties have been studied by a combination of in vitro analy- ses of human primary ECs and in vivo a...

Ngày tải lên: 18/02/2014, 17:20