Báo cáo khoa học: Dimerization of mammalian adenylate cyclases Functional, biochemical and ¯uorescence resonance energy transfer (FRET) studies pot

... PRIORITY PAPER Dimerization of mammalian adenylate cyclases Functional, biochemical and ¯uorescence resonance energy transfer (FRET) studies Chen Gu1, James J. Cali2 and Dermot M. F. Cooper1,31Neuroscience ... proteinmolecular masses of  120 kDa, again sugge sted a higherorder assembly of adenylate cyclases. Mammalian adenylate cyclases are in the family of ATP-binding cassette (ABC)transporters and share their ... N-terminus of AC8 and the C1 region of differentmolecules precluded the detection of FRET.DISCUSSIONThe present group of studies have convinced us that adenylate cyclases dimerize and that functional...

Tài liệu Báo cáo khoa học: Association of mammalian sterile twenty kinases, Mst1 and Mst2, with hSalvador via C-terminal coiled-coil domains, leads to its stabilization and phosphorylation doc

... 3D).Both phosphorylation of Sav and its increase inabundance were dependent of the ability of Sav and Mst to interact. Deletion of the coiled-coil domain, and thus the ability of the two proteins ... growth and apoptosis in Drosophila in vivo, primarily throughthe regulation of Yki activity.Hpo is a Drosophila orthologue of the Ste20-likekinases, and is most similar to mammalian Mst2. Mst1 and ... interaction of Mst with Sav is required, and might be sufﬁcient, forit to increase levels of Sav.The levels of N-terminally truncated mutants of Sav that retained the C-terminal coiled-coil domain,and...

Báo cáo khoa học: Effects of cardiomyopathic mutations on the biochemical and biophysical properties of the human a-tropomyosin docx

... cosedimentation assay, in the absence and in t hepresence of troponins. In the absence of troponins, binding of Tms to actin was very weak and only small amounts of Tm wer e detected in gels after ... stability of human TmCD and DSC experiments were used as methods forevaluating the effect of mutations on the stability of Tms[34]. The thermal-induced unfolding of the rabbit [35–37],rat, and ... theoverall stability of the protein as meas ured by circ ulardichroism [ 16], and s howed alterations in the k inetics of contractile force g eneration [17]. Studies with m utationsA63V and K70T reported...

Báo cáo khoa học: Control of mammalian gene expression by amino acids, especially glutamine potx

... existence of a large variety of targetgenes involved in major functions in the organism[15,82,83]. Tables 4 and 5 and Fig. 2 illustrate both thediversity of the studies into the effect of glutamine ... J & Tunon MJ(2008) Effects of glutamine on proinﬂammatory geneexpression and activation of nuclear factor kappa B and signal transducers and activators of transcriptionin TNBS-induced ... inhibitory effect of some amino acids. Moreover, aminoacids exert a wide range of effects via the activation of different signallingpathways and various transcription factors, and a number of cis elementsdistinct...

Báo cáo khoa học: Binding of the volatile general anesthetics halothane and isoﬂurane to a mammalian b-barrel protein doc

... Grolli5 and Roderic G. Eckenhoff1,31 Department of Anesthesia, University of Pennsylvania, Philadelphia, PA, USA2 Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, ... halothane and isoﬂurane bound with Kdvalues of 80 ± 10 lm and 100 ± 10 lm, respectively. Halothane and isoﬂuranebinding resulted in an overall stabilization of the folded conformation of the ... the effect of bound halothane and isoﬂuraneon global protein dynamics, with the goal of furtherdeﬁning a potential mechanism of volatile generalanesthetic action.ResultsBinding of the volatile...

Báo cáo khoa học: Transcription of mammalian cytochrome c oxidase subunit IV-2 is controlled by a novel conserved oxygen responsive element pptx

... in the upper band, but does not affect the intensity of the lower (speciﬁc) band. The Sp1 probe shows threebands, with the lowest band migrating at the position of the upper band obtained with ... lung and liver under standardconditions as described previously [17]. Three micromolarCcO was dialyzed in the presence of 0.1 mm ATP and 120 lm cardiolipin to remove cholate and to replace poten-tially ... Beyer C & Arnold S (2006) Effect of hypoxiaon the transcription pattern of subunit isoforms and thekinetics of cytochrome c oxidase in cortical astrocytes and cerebellar neurons. J Neurochem...

Tài liệu Báo cáo khoa học: Basis of recognition between the NarJ chaperone and the N-terminus of the NarG subunit from Escherichia coli nitrate reductase pdf

... site with binding stoichiome-try (n) of 0.3 ± 0.2 and a binding constant (Kd )of 3.4 ± 4 · 10)9m and a second with a stoichiometry of 0.7 ± 0.1 and a Kd of 3.3 ± 3 · 10)7m (Fig. 5A).Identical ... 4C).The decrease in some of the free state resonances and the appearance of new resonances upon complexformation indicated a slow exchange on the NMRtimescale between the free and the bound forms ... available:Fig. S1. Overlay of 1H,15N-HSQC spectra at 300 K of NarG(1–28) peptide in the absence (black trace) and inthe presence (orange trace) of 2 molar ratio of NarJ.Fig. S2. Overlay of 1H,15N-HSQC...

Tài liệu Báo cáo khoa học: Roles of heat shock factors in gametogenesis and development pptx

... over-expres-sion of Hsp60 and Hsp70 and exhibit abnormal mor-phology in correlation with increased proliferation,premature differentiation and elongation, inhibition of denucleation and of loss of organelles ... mechanisms for the lack of HSF2. In line withthis hypothesis, double disruption of Hsf1 and Hsf2is associated with sterility and complete arrest of spermatogenesis [72].Elucidation of HSF2 function ... weeks [106].Interplay of HSF1 and HSF4 in lens epithelial and lens ﬁber cells and in olfactory neurogenesisExamination of the double Hsf1Hsf4 knockoutallowed the unravelling of a remarkable interplaybetween...

Tài liệu Báo cáo khoa học: Effect of siRNA terminal mismatches on TRBP and Dicer binding and silencing efﬁcacy pdf

... target, loading of the appropriate guidestrand into the RISC is critical. Improved understand-ing of the interactions of siRNAs with TRBP and Dicerwill enable improved design of siRNA therapeutics.In ... impact of silencingTRBP and Dicer on the function of the fully paired and mismatched sequences, we wished to examineFig. 1. Effect of guide strand 5¢ end mismatch on silencing efﬁcacy of siRNAs. ... examining internal and terminal modiﬁcations will identify design rules forenhancing the activity of siRNA duplexes, and also pro-vide a better understanding of the roles of TRBP and Dicer in controlling...

Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-speciﬁc blood vascular and lymphatic endothelial cells of the mouse pdf

... concentra-tion of G418 (Invitrogen) of 400 lgÆmL)1. G418-resistantcolonies were picked and expanded for PCR genotyping and the formation of embryoid bodies. One ESC clone(T26) out of 48 G418-resistant ... roles in develop-ment and in health and disease [1]. Their propertieshave been studied by a combination of in vitro analy-ses of human primary ECs and in vivo analyses of genetically modiﬁed ... further analysis of the molecular and cellular phe-notypes of endothelial cells and their heterogeneity at various developmen-tal stages, in vascular beds and in various organs has often been hamperedby...

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