Báo cáo Y học: Identification and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is specifically modulated in thyroid cells by cAMP pot

... 16, 545–550. 3. Matthews, H.R. (1995) Protein kinases and phosphatases that act on histidine, lysine, or arginine residues in eukaryotic proteins: a possible regulator of the mitogen -activated protein kinase ... A ˚ ke Engstro ¨ m at the Peptide Synthesis and Analysis Laboratory of the Department of Medical Biochemistry and Microbio- logy, Uppsala University. The SP6 p...

Ngày tải lên: 21/02/2014, 01:21

... is a homotetrameric protein consisting of 31-kDa subunits; it belongs to a family of lyases involved in carbohydrate metabolism. GatY is highly thermolabile and is degraded in vivo at temperatures ... including UspA itself (Fig. 2), and one larger protein consisting of two UspA domains in tandem [17]. The small members of this family are proteins of 142–144 a...

Ngày tải lên: 22/02/2014, 07:20

... pH 6 and pH 8, 80% activity remained. The N-D-AAase had higher hydrolysing activity against N-ace- tyl- D -amino acid derivates containing D -methionine, D -leucine and D -alanine and against ... amidohydrolase; Alicaligenes faecalis-DA1: Alcaligenes faecalis DA1 N-acyl– D -amino acid amidohydrolase; V. paradoxus Iso1: Variovorax paradoxus Iso1 N-acyl- D -amino acid amidohydrolase; P....

Ngày tải lên: 08/03/2014, 16:20

... Identification and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is specifically modulated in thyroid cells by cAMP Hortensia Mircescu 1 *, ... HR1-Rho -binding domain, and between amino acids 522 and 579, the PROFILE SCAN program identifies a PDZ domain showing 30% identity with the...

Ngày tải lên: 23/03/2014, 21:20

... CP2 binding of the two HXPR-motif -containing proteins, REST and YYI. Both REST and YY1 function as a transcription modulator by recruiting and utilizing HDAC family proteins [36,37,39]. REST acts ... (5¢-GAAACCATTTTGCAGCGAAAGTATACAC-3¢) for Pro ⁄ Arg to Ala ⁄ Ala mutations in bases 803–830. The two overlapping PCR fragments were mixed and added as a template in the sec...

Ngày tải lên: 16/03/2014, 18:20

... (Hoechst dye 33258) and calf thymus DNA as standard. Protein was determined by a modification of the Lowry method as described by Peterson using bovine serum albumin as standard [26]. Determination of ... (T in pig and human; M in rat), 614 (I in pig and human; L in rat), 680 (L in pig and human; I in rat), 760 (K in pig; R in rat and human), 991 (A in...

Ngày tải lên: 31/03/2014, 21:21

... STAT protein inter- action domain, STAT protein all-alpha domain, STAT protein DNA -binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain ... STAT dimers on adjacent DNA -binding sites, a coiled-coil STAT protein all-alpha domain, which is implicated in other protein protein interactions, a STAT p...

Ngày tải lên: 16/02/2014, 09:20

... on LacZ filter-lift assay in less than 1 day, and > 7.5 b-gal units in liquid LacZ assay). AD, Gal4 activation domain; BD, Gal4 binding domain. Yeast two-hybrid Yeast three-hybrid SmSmad4-BD SmTbRI-BD ... comprising only 42 amino acids. The N-terminal MH1 domain of SmSmad1B consists of 137 amino acids, and comprises a conserved nuclear localization signal and a b-ha...

Ngày tải lên: 18/02/2014, 16:20

... modular structure: a conserved DNA -binding domain (DBD) that contains two zinc finger motifs binding to the cis-regulatory region of a target gene, and a conserved ligand -binding domain (LBD) that ... acts as a critical partner and thus plays a central role in a variety of nuclear signaling pathways [3–6]. Schistosoma mansoni is a multicellular eukaryotic pa...

Ngày tải lên: 07/03/2014, 11:20

... i.e. by reducing and detoxifying H 2 O 2 , peroxynitrite and numerous organic hydroperoxides. They are ubiquitous enzymes found in every domain of life such as eukarya, bacteria and archaea. Prxs ... and mechanistic data actually support the division of 2-Cys Prxs into two subclasses, ‘typical’ and ‘atypical’ 2-Cys Prxs. All three Prxs classes are found to have in common...

Ngày tải lên: 07/03/2014, 12:20