Báo cáo khoa học: Dimerization and oligomerization of the chaperone calreticulin pptx

... complete unfolding of the protein. Oligomerization of calreticulin As mentioned above, besides induction of dimerization, 2.7 M urea also induced a small degree of oligomerization of calreticulin. ... added to the calreticulin sample, and the size of this peak remained unchanged throughout the experi- ment, confirming that the reduction in the monomeric ca...

Ngày tải lên: 23/03/2014, 21:20

... advantage of p53 mutation analysis, and a unique feature of this database, is the availabil- ity of the residual activity of the majority of p53 mis- sense mutants. The biological activity of mutant ... prediction of the severity of p53 mutants. The method was trained on a set of known mutants and subsequently evaluated on another set. The method is based o...

Ngày tải lên: 18/02/2014, 11:20

... N-terminal of the mature enzyme and by the ÔflapÕ. The anchorage of the prosegment and of part of the N-terminus in the active site cleft is made by ionic interactions established between Lys11/Tyr13 of ... Like prophytepsin, the precursor form of cyprosin was processed in different isoforms by the excision of the prosegment and of most of the PSI [2...

Ngày tải lên: 19/02/2014, 12:20

... (typically, the main verb or nominalization representing the event) and a set of arguments. When a name candidate is in- volved in an event, the trigger word and other arguments of the event ... results too, such as the family name and given name of person, the prefixes of the abbreviation names, the modifiers and suffixes of organization names. Then t...

Ngày tải lên: 20/02/2014, 12:20

... with the gene of the benzaldehyde lyase and for helpful discus- sions, the teams of the Swiss Light Source (Villigen ⁄ CH) and of the EMBL outstation Hamburg for their help with data collection and ... important for cofactor binding and catalysis. The BAL residues are labeled and the BAL hydrogen bonds are displayed. The yellow and red residues at the top a...

Ngày tải lên: 07/03/2014, 12:20

... intensity of the bands may be the result of a different copy number of the genes in the hybridizing fragments. If so, the number of the bands do not represent the family size. Thus, the mlp gene ... Leu117) or Met (Met153 of the ML1p and ML2p B-chains and Met156 of the ML3p B-chain corresponding to the ricin B-chain Leu152; and Met234 of the ML1p a...

Ngày tải lên: 07/03/2014, 15:20

... experimentally. Indeed, the presence of Arg213 along with the proximity of the charge relay Asp260 within the active site could be responsible for the modification of the catalytic properties of the catalytic histidine. ... avor the displacement of the equilibrium toward the neutral state of His290. To attest this hypothesis, an Arg213Ala mutant has been express...

Ngày tải lên: 07/03/2014, 16:20

... were present in the 3¢-UTR as well as in the 5¢-UTR, and four potential poly(A) signals were found in the 3¢-UTR (Fig. 1), two of them just 14 or 23 bp upstream of the poly(A) tail. The remaining ... organization resembles that of mammalian IL-11. The sequences between the trout cDNA and genomic DNA in the coding region are identical, although there were differ- ence...

Ngày tải lên: 07/03/2014, 16:20

... these 21 consonants. On the other hand Regime 2 of Figure 3(a) as well as Regime 1 of Figure 3(b) comprises of the rest of the consonants. The point marked as x in both the figures indicates the ... corresponding to the nodes in the set V L and the other corre- sponding to the nodes in the set V C . Degree distribution of the nodes in V L : Fig- ure 2 shows...

Ngày tải lên: 08/03/2014, 02:21

... in the presence of Gd 3+ at pH 4.0, but the degree to which the intensities recovered was lower in the presence of Gd 3+ , particularly for the C-terminal residues, than in the presence of the ... suggests that the C-terminus of the peptide is partially embedded into SDS micelles at pH 4.0, which may therefore block the entrance of either solvent molecules or met...

Ngày tải lên: 16/03/2014, 16:20