Báo cáo khoa học: Deflavination and reconstitution of flavoproteins Tackling fold and function pot

... FEBS 2003 REVIEW ARTICLE Deflavination and reconstitution of flavoproteins Tackling fold and function Marco H. Hefti*, Jacques Vervoort and Willem J. H. van Berkel Laboratory of Biochemistry, Wageningen ... Reversible dissociation of flavoproteins into apo- protein and flavin prosthetic group yields valuable insights in flavoprotein folding, function and mechani...

Ngày tải lên: 23/03/2014, 21:20

... N e . The crystal structures of K328 ⁄ K328A–C12 and K328R and the results of the decarboxylation assays of the ‘bulky’ mutants all point to the orientation of H333 and its hydrogen bond donor ... crystal structures of both CHH and CHN enzymes [1,4,5,11,12] have contributed to the under- standing of the role of conserved residues in the active site of CHH condensing en...

Ngày tải lên: 19/02/2014, 08:20

... comparison of band absorbance. Absence of a sharp and convoluted spectral band around 3600–3000 cm )1 in the spectra of both chitosan and LMWC indicated the absence of free -OH groups and the involvement ... from the offal of marine food processing industry (and pronase), and show the biofunctionalities of these materials. Acknowledgements Authors thank Dr S. Subramanian...

Ngày tải lên: 19/02/2014, 12:20

... reflecting gene expression. With a total of 600–670 spots per gel and a reproduci- bility of spot assignment between different gels of 89.4–99%, we detected one spot among the 48 signals that showed ... disorders of glycosylation IIc and IIf [100,101], (b) monitor other cell and suppressor types to define the range and specificity of the detected effect, (c) delineate the intra...

Ngày tải lên: 16/03/2014, 10:20

... inhibitor of glucosidases I and II. In the presence of castanospermine, the levels of both Glc1Man9GlcNAc2 and peak X were increased (Fig. 2A,B). Under these conditions, the decreasing amounts of Man9GlcNAc2 ... action of glucosi- dase I and II or by the soluble UDP-Glc–glycoprotein glucosyltransferase (UGGT) can be ligands for the mole- cular lectin-like chaperones calnexin...

Ngày tải lên: 16/03/2014, 16:20

... Levin 2 1 Laboratory of Cell Biology, National Heart, Lung, and Blood Institute and 2 Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, ... spectroscopic bandwidths are functions of dynamic parameters derived from intermolecular and intramolecular motions. Band shapes are often analyzed in the c...

Ngày tải lên: 17/03/2014, 03:20

... 3.4.21.76) and CG [20,21,23]. However, to date, no in depth investigation of the S¢ specificity of CG has been carried out that could aid the understanding of its pathophysiological function, and distinguish ... result of the presence of negatively-charged residues at P2 and P2¢ that are not accommodated within the b2-tryptase active site because of the presence of Asp147...

Ngày tải lên: 28/03/2014, 22:20

... conformers of discrete and similar free energy, and that binding of ligands shifts the equilibrium in favour of the functional conformer [62]. If this view is correct, the hysteretic behaviour of enzymes must ... k cat /K m for the hydrolysis of BzSCh was 17 -fold and 32 -fold lower than that for hydrolysis of BzCh by wild-type and D70G, respectively. The rate-limiting s...

Ngày tải lên: 07/03/2014, 14:20

... pur- pose of the manual classification is (i) to evaluate the reliability and performance of the clustering experiments on a preliminary set of verbs, and (ii) to explore the potential and limit ... idiosyncratic properties of the verbs and the specific attributes of the desired verb classification. 1 Introduction The verb is central to the meaning and the struc- ture of...

Ngày tải lên: 17/03/2014, 22:20

... Problems of erroneous grammar or ambiguity are solved by re- ferring to a lexicon and a grammar, re-generating substitutes expressions of the original sentence, and the user deciding which one of the ... real human-human trivial dialogues (obtained from the corpus of the University of South Califor- nia (2005)) and from the hand crafted ALICE 4 In this paper DB stands for data...

Ngày tải lên: 23/03/2014, 18:20