Báo cáo khoa học: Deflavination and reconstitution of flavoproteins Tackling fold and function pot
Báo cáo khoa học: Deflavination and reconstitution of flavoproteins Tackling fold and function pot
... FEBS 2003
REVIEW ARTICLE
Deflavination and reconstitution of flavoproteins
Tackling fold and function
Marco H. Hefti*, Jacques Vervoort and Willem J. H. van Berkel
Laboratory of Biochemistry, Wageningen ... Reversible dissociation of flavoproteins into apo-
protein and flavin prosthetic group yields valuable insights in
flavoprotein folding, function and mechani...
Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt
... N
e
. The crystal structures of
K328 ⁄ K328A–C12 and K328R and the results of the
decarboxylation assays of the ‘bulky’ mutants all point
to the orientation of H333 and its hydrogen bond
donor ... crystal structures of both CHH and CHN
enzymes [1,4,5,11,12] have contributed to the under-
standing of the role of conserved residues in the active
site of CHH condensing en...
Tài liệu Báo cáo khóa học: Non-specific depolymerization of chitosan by pronase and characterization of the resultant products pptx
... comparison of
band absorbance. Absence of a sharp and convoluted
spectral band around 3600–3000 cm
)1
in the spectra of both
chitosan and LMWC indicated the absence of free -OH
groups and the involvement ... from
the offal of marine food processing industry (and pronase),
and show the biofunctionalities of these materials.
Acknowledgements
Authors thank Dr S. Subramanian...
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... reflecting gene expression.
With a total of 600–670 spots per gel and a reproduci-
bility of spot assignment between different gels of
89.4–99%, we detected one spot among the 48 signals
that showed ... disorders of glycosylation IIc and IIf
[100,101], (b) monitor other cell and suppressor types
to define the range and specificity of the detected effect,
(c) delineate the intra...
Báo cáo khóa học: Endoplasmic reticulum-associated degradation of glycoproteins bearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5 CHO cell line docx
... inhibitor of glucosidases I
and II. In the presence of castanospermine, the levels of
both Glc1Man9GlcNAc2 and peak X were increased
(Fig. 2A,B). Under these conditions, the decreasing
amounts of Man9GlcNAc2 ... action of glucosi-
dase I and II or by the soluble UDP-Glc–glycoprotein
glucosyltransferase (UGGT) can be ligands for the mole-
cular lectin-like chaperones calnexin...
Báo cáo khoa học: Purple membrane lipid control of bacteriorhodopsin conformational flexibility and photocycle activity An infrared spectroscopic study docx
... Levin
2
1
Laboratory of Cell Biology, National Heart, Lung, and Blood Institute and
2
Laboratory of Chemical Physics,
National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, ... spectroscopic bandwidths are functions
of dynamic parameters derived from intermolecular and
intramolecular motions. Band shapes are often analyzed in
the c...
Báo cáo khoa học: Discriminating between the activities of human cathepsin G and chymase using fluorogenic substrates potx
... 3.4.21.76)
and CG [20,21,23]. However, to date, no in depth
investigation of the S¢ specificity of CG has been
carried out that could aid the understanding of its
pathophysiological function, and distinguish ... result of the presence
of negatively-charged residues at P2 and P2¢ that are
not accommodated within the b2-tryptase active site
because of the presence of Asp147...
Báo cáo khóa học: Damped oscillatory hysteretic behaviour of butyrylcholinesterase with benzoylcholine as substrate potx
... conformers of discrete
and similar free energy, and that binding of ligands shifts the
equilibrium in favour of the functional conformer [62]. If
this view is correct, the hysteretic behaviour of enzymes
must ... k
cat
/K
m
for the
hydrolysis of BzSCh was 17 -fold and 32 -fold lower than that
for hydrolysis of BzCh by wild-type and D70G, respectively.
The rate-limiting s...
Báo cáo khoa học: "Experiments on the Choice of Features for Learning Verb Classes" potx
... pur-
pose of the manual classification is (i) to evaluate
the reliability and performance of the clustering
experiments on a preliminary set of verbs, and (ii)
to explore the potential and limit ... idiosyncratic properties of
the verbs and the specific attributes of
the desired verb classification.
1 Introduction
The verb is central to the meaning and the struc-
ture of...
Báo cáo khoa học: "Towards Web-Based Evaluation of Automatic Natural Language Phrase Generation" potx
... Problems of
erroneous grammar or ambiguity are solved by re-
ferring to a lexicon and a grammar, re-generating
substitutes expressions of the original sentence,
and the user deciding which one of the ... real
human-human trivial dialogues (obtained from
the corpus of the University of South Califor-
nia (2005)) and from the hand crafted ALICE
4
In this paper DB stands for data...