Báo cáo khoa học: PKCd-dependent cleavage and nuclear translocation of annexin A1 by phorbol 12-myristate 13-acetate pdf

... PKCd-dependent cleavage and nuclear translocation of annexin A1 by phorbol 12-myristate 13-acetate Yoon S. Kim 1, *, Jesang Ko 2, *, In S. Kim 1 , ... cleavage and nuclear translocation of ANX-1. HEK 293 cells were preincubated in the presence of rottlerin or Ro-31-8425, and were stimulated with PMA. The cleavage and nuclear translocation of ANX...

Ngày tải lên: 23/03/2014, 21:20

... mouse, and a second pair (cSc and cSd) belong to an earlier offshoot. This may indi- cate that there were two cS genes in the common ancestor of fish, birds and mammals and that only one of these ... USA). All use of animals complied with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research and the Intramural Animal Care and Use program of the Nat...

Ngày tải lên: 19/02/2014, 17:20

... International Institute of Molecular and Cell Biology, Warsaw, Poland 5 PhD School, Institute of Biochemistry and Biophysics PAS, Warsaw, Poland 6 Institute of Molecular Biology and Biotechnology, ... temperature and terminated after different time intervals by the addition of 1 mm phenylmethanesulfonyl fluoride (for trypsin and chymotrypsin digestions), or by addition o...

Ngày tải lên: 29/03/2014, 08:20

... s )1 . The lowest R 2 values are shown by the N-terminal and C-terminal residues and by Leu34, Gln44, Arg53, Arg58 and Gln59. A unique value of 26.39 s )1 , by far the highest one, is observed for Tyr25, ... dis- tribution of the energy between the overall tumbling and the internal mobility, and is indicative of the degree of internal restraint of each 15 N– 1 H vect...

Ngày tải lên: 18/02/2014, 16:20

... supported by O i -HN i +3 hydrogen bonds for Asn11(CO)–Trp14(HN) and Ser12(CO)– Thr15(HN), and confirmed by the slow solvent exchange kinetics of the amide protons of Trp14 and Thr15. The observation of ... Pro, Val or Lys, c-carboxylation of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of...

Ngày tải lên: 18/02/2014, 17:20

... Soares RM (2006) Secretion of serine peptidase by a clinical strain of Candida albicans: influence of growth conditions and cleavage of human serum proteins and extracellular matrix components. FEMS ... and the CFU counted. The per- centage of surviving CFU was calculated by the formula N ⁄ N control · 100, where N and N control were the numbers of CFU obtained after...

Ngày tải lên: 18/02/2014, 17:20

... (Q31–Q245) and their quality was assessed by using procheck v. 3.5.4 [40] and the 3D profile of insightii (Accelrys Software Inc., San Diego, CA, USA). The best model was completed by addition of all hydrogen ... concentration of Rep245 pre- pared in 10 mm sodium phosphate (pH 7.4) was 5 lm. CD spectra were recorded at 60, 70 and 80 °C between 190 and 260 nm with a step incre...

Ngày tải lên: 18/02/2014, 18:20

... from 6.5 to 8.0, a K m of 6.5 lm, and a k cat of 1.0 s )1 . ACMSD I is inhibited by quinolinic acid, picolinic acid and kynurenic acid, and it is activated slightly by Fe 2+ and Co 2+ . Site-directed ... amplification and cloning of ACMSD isoforms Human brain, liver and kidney total RNA (Clontech Laboratories, Inc., Mountain View, CA, USA) was reverse transcribed in the p...

Ngày tải lên: 19/02/2014, 02:20

... catalytic proper- ties of HpSDH and the effects of pH and temperature on HpSDH. The results show that HpSDH has a k cat of 7.7 ± 0.9 s )1 , K m of 0.148 ± 0.028 mm and k cat ⁄ K m of 5.2 · 10 4 m )1 Æs )1 toward ... shikimate, k cat of 7.1 s )1 and K m of 0.182 mm toward NADP, k cat of 5.2 s )1 and K m of 2.9 mm toward NAD. The optimum pH of the enzyme activity i...

Ngày tải lên: 19/02/2014, 05:20

... domains and have a similar mode of activation [1]. There are two isoforms of S6 kinase, S6K1 and 2. Both have highly homologous kinase and kinase extension domains flanked by the less conserved N- and ... is activated by an array of mitogenic stimuli and is a key player in the regulation of cell growth. The activation process of S6 kinase involves a complex and sequen...

Ngày tải lên: 19/02/2014, 07:20