Báo cáo khoa học: Structural analysis of lipopolysaccharides from Haemophilus influenzae serotype f Structural diversity observed in three strains ppt
Báo cáo khoa học: Structural analysis of lipopolysaccharides from Haemophilus influenzae serotype f Structural diversity observed in three strains ppt
... protein. J. Exp. Med. 187, 631–640.Ó FEBS 2003 Structural diversity of LPS in H. in uenzae (Eur. J. Biochem. 270) 3167 Structural analysis of lipopolysaccharides from Haemophilus in uenzae serotype ... representativeofthediversitywithinthetypefcluster.Previous studies of LPS from H. in uenzae haveresulted in a structural model consisting of a conservedFig. 4. Selected region of the two-dimensionalNOESY ... to report structural details on LPS from sero-type f strains. We found that the LPSs of all strains werehighly heterogeneous mixtures of glycoforms expressing thecommon H. in uenzae structural...
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Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf
... have investigated the import-ance of the amino acid sequence of a-helix F (hF) and theconnecting loop to s3A (hF/s3A-loop) for the rate of latencytransition. We grafted regions of the hF/s3A-loop ... from the amount of PAI-1 required to inhibit half the uPA. The half-life of PAI-1 was finally calculated from an exponential decay plot of the data obtained. Generally, only one preparation of each ... consensus serpin sequence of thehF/s3A-loop is included in Table 1 (adapted from [2]).Alignment of the three structures was performed accord-ing to the Caatoms in the rigid serpin fragment 2c...
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Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx
... suggested to be linked toearly stages of PD pathogenesis [26]. Interestingly, in our model, dopamine induces an increase of the actinbundles regulator fascin 1 [27] and discordant changes of two calcium-dependent, ... of ββ-Actinα-SynucleinFig. 1. Relative expression of a-synuclein in b-gal and a-syn cells in response to dopamine (DA) treatment. Results are indicated as thefold of induction relative to expression observed in ... of four proteins [stathmin 1, glutathione S-transferase(GST)p, Ran 1 binding protein and C1q binding pro-tein], related to cell signaling, apoptosis and cytoskele-ton regulation (Fig. 2, insets;...
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Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf
... actual binding site of serotonin cannot be inferred from these results. Although serotonin can bind to theactive site binding locus (W82), binding to PAS cannotbe ruled out. If serotonin binds ... enzyme in its activatingcapacity for neutral substrates (0.8–1.0 mm). The sameis true for the binding of tyramine to D70G, althoughthe affinity of D70G for tyramine is weaker than theaffinity of ... can be tenta-tively interpreted in terms of allosteric inhibition ⁄ acti-vation: the binding of tyramine to PAS induces aconformational change that affects the formation of the productive enzyme–substrate...
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Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc
... (Fig. 1). FI tracesobtained from thylakoids were composed of three well-distinguished phases in terms of amplitude and half-time (Table 1). In contrast with the I peak observed in FI of intactleaves, ... blocking electron transfer from QAto QB[21,22], is of importance for modulatingthe dynamics of PQ pool reduction and determining itseffect on FI kinetics as discussed below. The increase in AO–J observed ... apparentactivation energy (EA) of each FI kinetic step from itsrate constant. Our results indicate a differentbioenergetic origin for each kinetic step of the FI rise,as the steps have different apparent...
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Tài liệu Báo cáo khoa học: Thermodynamic analysis of porphyrin binding to Momordica charantia (bitter gourd) lectin pptx
... spectraobtained by subtracting the spectrum of porphyrin alone from the spectra obtained in the presence of differentconcentrations of the lectin are s hown in Fig. 2B. Thedifference spectra ... Waals ’ interactions and stacking of aromatic side chains with the porphine core of theporphyrins, as observed in the jacalin–H2TPPS interaction,most likely favor the binding of porphyrins to ... thatbinding of the same porphyrin to jacalin is mediated by acombination of hydrogen bonding and nonpolar inter-actions, including aromatic stacking interactions betweenthe phenyl rings of the...
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Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf
... concentration o f 50 lg of p rotein per IEF strip forsilver staining, hot Coomassie s taining, ÔStains-AllÕ labellingor immunoblotting. T he following running conditions wereused: 60 m in at 1 00 ... e ffect of thedeficiency in dystrophin o n the expression of Ca2+-bindingproteins. The relatively unique combination o f the pI valueand molecular mass of individual 2D spots can be useful ... drastically reduced in mdxpreparations. This finding agrees with both t he 2D ÔStains-AllÕ analysis of Fig. 3 and the 1D i mmunoblotting of Fig. 4.As the full-length Dp427 isoform of dystrophin does notenter...
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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt
... the bin ding to the E-box.Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box.However, substitution of His57 for Ala did not affect ... (BMAL1:112–626) did not affect thebinding of BMAL1 to DEC1; deletion of 235 amino acids(BMAL1:236–626) slightly diminished the binding,although a strong binding ability of BMAL1:236–626 withDEC1 ... 139 of DEC1(including the bHLH domain) is sufficient for thesuppressive activity of DEC1. The C-terminal region(amino acids 140–412), including the Orange domain[18], i s not required for...
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Tài liệu Báo cáo khoa học: Fluorescence analysis of the Hansenula polymorpha peroxisomal targeting signal-1 receptor, Pex5p pdf
... This is indeed the case as can be concluded from the structural model of the PTS1-bindingC-terminal domain of HpPex5p (Fig. 4). In this domain three of the seven Trp residues of HpPex5p are found.However, ... residues254–568 of HpPex5p, corresponding to the C-terminalhalf of the protein including all TPR repeats (Fig. 4). Addition of the L2 peptide (dansyl-GSKL) was safelypossible due to the conserved binding ... andtherefore unlikely to be involved in FRET.The three- dimensional structure of the TPR domain of human Pex5p (HsPex5p) [11] revealed that the PTS1binding site is formed by two clusters of three...
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Báo cáo khoa học: Thermodynamic analysis of Jun–Fos coiled coil peptide antagonists pdf
... consists of heterodimers of the Jun (e.g. cJun,JunB, JunD) and Fos (e.g. cFos, FosB, Fra1, Fra2)families of proteins. Different homologues combine toform different heterodimers, which in turn ... results in a positive (unfavourable) netcontribution to the overall free energy of binding. DSconfarises from a reduction in conformational degrees of free-dom of backbone and side chain atoms ... to the free energy of bind-ing. Indeed, the free energy of binding is 2–3 kcalÆmol)1less than any of the antagonist–cJun or antagonist–cFos complexes. ITC data collected from the leucinezipper...
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