Báo cáo khoa học: Disease-related mutations in cytochrome c oxidase studied in yeast and bacterial models pptx

... mut GCCTTTCTCGGGGCCGCTTCAGCGTGGGAACG P Sp1 middle mut GGCGCCCGCCCCCGGCCATACCACAGCCTTTCTCGG P Sp1 proximal mut GCGGGCGCCCGAACCCTGCCCGCCCCACA P intron IIIfoward ATATTCTAGGATCCTGGCTCATTCACTGCTGTCAC P intron IIIreverse ATATTCTAGGATCCCGGCTTCCCCCTCCCTGCAG P HIF-1a ... reverse CTCGCGGGCTCGGCAGTGGGAG P prom+1 AGTCTATTCTCGAGCACCTGGGACTACAGG P prom+2 AGTCTATTCTCGAGCCCAAAGCGCTGAGATTACAG P prom+3 AGTCTAT...

Ngày tải lên: 30/03/2014, 03:20

... for protons [21]. Mutations in mitochondrially encoded subunits of cytochrome oxidase in humans: correlating pathogenicity to the biochemistry elucidated in yeast and bacterial models Mitochondrial genes ... & Rich, P.R. (1993) Rapid screening of cytochromes of respiratory mutants of Saccharo- myces cerevisiae – application to the selection of strains containing novel...

Ngày tải lên: 23/03/2014, 21:20

... structure of cytochromes c exhibits clear differ- ences from that of the well-known Ambler’s class I cytochromes c. The class I cytochromes c are spherical proteins with a hexacoordinate heme covalently ... thermoluteolus cyto- chrome c (PHCP) and H. thermoluteolus cytochrome c 552 (PH c 552 ) genes are indicated as c and c 552 , respectively. The arrow and arrowhead indic...

Ngày tải lên: 06/03/2014, 00:20

... nucleotides 50–96 and the second intron included nucleotides 210–253 in each gene. Excluding introns and stop codons, the c- T1 and c- T2 coding regions were each 1383 bp in length and predicted proteins ... testing via site-direc- ted mutagenesis and functional analysis of c- tubulins in several model systems, including the yeasts Saccharo- myces cerevisiae and Schizosac...

Ngày tải lên: 07/03/2014, 04:20

... fluorescence quenching induced by each peptide (Fig. 6) echoes the colorimetric and SR data. Magainin, for example, induced the fastest quenching among the three peptides in both vesicle models ... reflecting its pronounced lipid–water interface binding and interactions. The hydrophobic sequence KAL, on the other hand, seemed to affect the fluorescence quenching to a much lesser degree co...

Ngày tải lên: 30/03/2014, 20:20

... (or CD) and AC (or BD) interfaces, as illustrated in Fig. 2A. The AB interface is dominated by hydrophobic interactions, whereas the AC interface is comprised predominantly of salt bridges and ... 382 nm in 1 m HCl [39]. For yellow FP, Venus, the EC of the chromophore was back-calculated using 22 000 m )1 Æcm )1 at 280 nm in 10 mm Tris ⁄ HCl. Fluorescence spectroscopy Fluorescence e...

Ngày tải lên: 06/03/2014, 11:20

... as controls (gray). Cells were stained with FlAsH (B) or CrAsH (C) , and imaged under the confocal microscope. In cells that express TCC (Bb and Cb) and TCN (Bc and Cc), FlAsH and CrAsH selectively ... structure, with characteristic minima at 210 nm and 222 nm in the far-UV CD spectrum. The spectra of TCC, TCN and TCL overlap the spectra of mPrP, indicating that the secondar...

Ngày tải lên: 06/03/2014, 11:20

... studied naturally occurring and recombinant protein C Gla domain variants for soluble (s)EPCR binding, cell surface activation to activated protein C (APC) by the thrombin–thrombomodulin complex, and phospholipid dependent ... 7.2–115 n M). (C) Complete EPCR ⁄ protein C binding cycle. 1, Wildtype sEPCR (800 ng) was injected across the flow cell of a CM5 sensor chip coated with RCR-...

Ngày tải lên: 16/03/2014, 18:20

... morocco confirm that spain declined to aid morocco that spain declined to aid morocco spain declined to aid morocco declined to aid morocco to aid morocco aid morocco morocco spain declined to confirm ... that spain declined to aid morocco declined to aid morocco confirm that spain declined to aid morocco aid morocco that spain declined to aid morocco spain declined to confirm that spain decl...

Ngày tải lên: 17/03/2014, 05:20

... sentence containing “Milan Kucan” has a higher score than a sentence contains only either Milan or Kucan. A sentence containing both Milan and Kucan but not in consecutive order gets a lower score ... the individual key concepts available, we proceed to cluster these concepts in order to identify major subtopics within the main topic. Clusters are formed through strict lexical c...

Ngày tải lên: 17/03/2014, 08:20