Báo cáo khoa học: Biosynthesis of riboflavin in Archaea 6,7-Dimethyl-8-ribityllumazine synthase of Methanococcus jannaschii pdf

... analyses in or- der to determine to what extent contextual informa- tion helps in discriminating between agreement and disagreement. By integrating the interpretation of the pragmatic function of an ... baseline algorithm. 3.2 Maximum Entropy Ranking We view the problem as an instance of statisti- cal ranking, a general machine learning paradigm used for example in statistical par...

Ngày tải lên: 20/02/2014, 16:20

... Stimulation of this signaling pathway also enhances metabolism, resulting in an increase in glucose uptake and an up-regulation of glycolysis [37,38]. In addition, PI3K signaling has been shown to be involved ... up-regulation of glucose metabo- lism and the induction of lipogenesis [37–40]. We hypothesized that signaling via the PI3K ⁄ Akt pathway was involved in the promotio...

Ngày tải lên: 07/03/2014, 00:20

... quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254. 24 Weber K, Pringle JR & Osborn M (1972) Measurement of molecular ... theoretical pI of 7.73; bcp4 encodes a putative pro- tein of 156 amino acids with a predicted molecular mass of 17 461.28 Da and a theoretical pI of 7.65; bcp3 encodes a putative pro...

Ngày tải lên: 07/03/2014, 05:20

... provide information on the local repartition of the angles involved in the SRE fos helix bending. Relative effect of bending strain There are several indications of a redistribution of the strains ... the bend: partial unstacking of some adenine, thymine and guanine bases and a more distinct presence of 2¢-endo conformations of furanose rings at 10 °C against a higher percenta...

Ngày tải lên: 07/03/2014, 09:20

... mine the identity and location of the nucleotide insertion. Four mutants obtained contained in- frame insertions resulting in the insertion of five amino acids into the Cdc37 protein. Stop ... pombe protein in a strain deleted for the endogenous cdc37 gene. Expression of a construct consisting of only the N-terminal domain of S. pombe Cdc37, lacking the postulated heat-shock pro...

Ngày tải lên: 16/03/2014, 22:20

... loss in C4H activity, does not affect I2C hydroxylation. As initial cinnamate binding is not in u- enced by this mutation (Table 2) and binding kinetics are first-order (indicating a single binding-site), ... affect the binding of cinna- mate and the coupling of the hydroxylation reaction [9]. This effect was probably due to a direct interaction of its side-chain with the aromatic rin...

Ngày tải lên: 17/03/2014, 10:20

... rate of inactivation was followed by periodically removing samples (10–50 lL) for assay of enzymatic activity. Initial rates of inactivation were deduced from plots of log (% of activity remaining) ... mechanisms [42,44]. The study of the effect of pH on enzyme inactivation allows the calculation of the pK a of the His176 side chain involved in the inactivation reaction. T...

Ngày tải lên: 23/03/2014, 12:20

... precursors in cutin synthesis [4]. The inde- pendence from cofactors in combination with, in some cases, high catalytic efficiencies and enantio- selectivities has created an interest in using epoxide hydrolases ... catalyzing the hydrolysis of alkyl and aryl epoxides into the corresponding vicinal diols [1,2]. They fulfill various functions in host organisms, including detoxificatio...

Ngày tải lên: 30/03/2014, 02:20

... standards; lane A, CBB staining; lane B, Stains-all staining; lane C, negative staining; lane D, Methyl green staining. Arrows on the right side of the lanes indicate the position of the 52 kDa component. ... DH & Jorgensen AO (1983) Staining of the Ca 2+ -binding proteins, calsequestrin, calmodulin, troponin C, and S-100, with the cationic carbocyanine dye ‘Stains-all’. J Biol Chem...

Ngày tải lên: 30/03/2014, 04:20

... using a Na ¨ ıve Bayes (NB) or Support Vec- tor Machines (SVM) classifier To gain further insight into the variation of ac- curacy with the amount of data available, we also plotted the learning ... expressing their true opinion on the topic. Next, they were asked to prepare a second brief speech expressing the op- posite of their opinion, thus lying about their true beliefs about the to...

Ngày tải lên: 31/03/2014, 00:20