Báo cáo khoa học: The N-acetylglutamate synthase/N-acetylglutamate kinase metabolon of Saccharomyces cerevisiae allows co-ordinated feedback regulation of the first two steps in arginine biosynthesis potx

... mechanisms is shown in Fig. 1. The fact that the presence of benzoic acid introduces an independent ATP drain in cell metabolism may also be of interest to those studying the regulation of cell energetics ... particularly during the transient. During the entire observation period of 72 h after the shift in benzoic acid concentration, the increase in OUR and th...

Ngày tải lên: 07/03/2014, 04:20

... alone. The kinase- deficient mutant, OXI1 K45R , in which the lysine residue of the ATP bind- ing domain is mutated to arginine, still interacted with PTI1-4. These data indicate that the kinase ... tenth of the input was loaded on to the gel and represents the amount of HIS-tagged proteins used for the assay. The in vitro binding assays were repeated twice usin...

Ngày tải lên: 14/02/2014, 19:20

... to overconsumption of Ginkgo seeds, which are the main source of ginkgotoxin [1]. In addition to the seeds, which accumulate the toxin, ginkgotoxin has also been found in the leaves of Ginkgo biloba ... the antivitamin character of both compounds and explains a depletion of cofactor formation in the organism in the presence of the antivitamins. The kineti...

Ngày tải lên: 07/03/2014, 10:20

... relationships of the C-terminus of mGRK6 To examine the functional significance of the C-ter- minus of mGRK6-A, a mutant was generated (mGRK6-A M1) carrying serine residues instead of the cysteine residues ... loss of the ability of the protein to phosphorylate light-activated rhodopsin. Interestingly, gradual removal of the C-terminal-most 16 residues of mGRK6-...

Ngày tải lên: 07/03/2014, 12:20

... FEBS 2003 The N -acetylglutamate synthase/ N -acetylglutamate kinase metabolon of Saccharomyces cerevisiae allows co-ordinated feedback regulation of the first two steps in arginine biosynthesis Katia ... (both expressing the kinase protein) allow growth of the arginine- deficient mutant in the absence of arginine. On the other hand, plas...

Ngày tải lên: 23/03/2014, 21:20

... kDa in nonreducing SDS ⁄ PAGE gel, indicating the homogeneity of the preparation. Antibacterial assays The antibacterial activity of the proteins was investigated using log-phase bacteria first ... or yeast cells in phosphate buffer containing 0.3 m NaCl. Heparin binding assays The heparin-binding capacities of trappin-2 and elafin were assessed by affinity chromatography...

Ngày tải lên: 18/02/2014, 17:20

... sequence and branching information [31]. The charge state of a fragment ion is then identified by u sing the isotope profile, owing to the high resolution provided by the TOF mass analyser. The product-ion ... cm) in pyridinium-acetate buffer, pH 4.5 (4 mL of pyridine and 10 mL of AcOH in 1 L of water), and the eluate was monitored by a refractive index detector. Configur...

Ngày tải lên: 19/02/2014, 16:20

... substrate affinity, the C-terminal tail of TAP2 induces a conformation in NBD2 that is nonpermissive for ATP binding. The ADP-binding state of TAP2 allows binding of ATP to TAP1. We hypothesize that peptide binding ... The resulting ADP-binding state of TAP2 and the C-terminal tail of NBD1 allow binding of ATP to TAP1. Peptide binding leads to a change in the conform...

Ngày tải lên: 20/02/2014, 02:21

... thrombin show that the A-chain closely follows the contour of the catalytic B-chain, hinging the two interacting six-stranded bar- rel-like domains of the B-chain [17]. A well-structured network of ... flexibility of their side-chains to the solvent the smaller the intrinsic fluorescence of the enzyme. The smaller gain in the intrinsic fluorescence of DK9 up...

Ngày tải lên: 07/03/2014, 12:20

... among the members of the Lad1 terminal clade but which are functionally different from those present in other SDHs, are located at the facing rims of the two domains of the protein that form the ... in polyol binding in the SDH [25] are also absolutely conserved in Lad1, and thus cannot determine the binding efficacy of hexitols and pentitols. On the other h...

Ngày tải lên: 07/03/2014, 15:20