Báo cáo khoa học: Purification and characterization of three isoforms of chrysophsin, a novel antimicrobial peptide in the gills of the red sea bream, Chrysophrys major doc

... CLGAT 3a (5¢-GCTGTGGCTACGAATGAGGCCGCC-3) and CLGAT3b (5¢-AAGGGAGAGAAACCTGACCTTGCACTTG-3¢). The 5¢-RACE was performed using Marathon cDNA Amplification Kit (Clontech, Palo Alto, CA) according to the manufacturer’s instructions, ... (1986) Biosynthesis and metabolism of arginine in bacteria. Microbiol Rev 50, 314–352. 10 Davis RH (1986) Commpartmental and regulatory mechanisms in...

Ngày tải lên: 20/02/2014, 03:20

... 2000-fold increase in specific activity. Analysis of the lectin on SDS/PAGE gave a single band at apparent molecular mass of 34 kDa. The binding affinity of the lectin in the hemolymph of the freshwater ... NaCl/Tris containing 1 M NaCl and 10 mm CaCl 2 at 4 °C, until the A 280 of the effluent was 0.002. The column was transferred to a water bath at 32 °C an...

Ngày tải lên: 21/02/2014, 00:20

... behavior is typical of integral membrane proteins. The polypeptide with an apparent molecular mass of 53 kDa appears as a double band in unboiled samples (lanes A1 and B1). Table 1. N-Terminal ... major polypeptide bands with apparent molecular masses of 53, 34, 31, and 16 kDa after SDS/ PAGE (Fig. 1, lane A1 ). In the boiled sample, the 34-kDa polypeptide was only d...

Ngày tải lên: 21/02/2014, 03:20

... less than that of MHCE because a considerable amount of undigested bands remained in the digest after a 10 min incubation, and the 36.5 kDa band was not easily detected even after a 120 min incubation. ... either by ZHE1, MHCE or MLCE, and their SDS ⁄ PAGE patterns were com- pared. SDS ⁄ PAGE of the MHCE digest after a 10 and 40 min incubation gave two bands (43 a...

Ngày tải lên: 07/03/2014, 04:20

... translation showed that they hold the characteristic features of all known papain-class cysteine proteinases, and a phylogenetic analysis revealed the existence of several papain and chymopapain ... proteinases it can be deduced that the common ancestor of the genera Carica and Vasconcellea already contained at least two different cysteine pro- teinases in its latex (p...

Ngày tải lên: 07/03/2014, 11:20

... Kentaro Ogawa, Hiroko Saika, Keizo Yuasa and Masami Nagahama Department of Biological Science and Technology, University of Tokushima Graduate School, Japan Genome analysis has indicated that plants, ... 5¢-ATGGCTGTTTACAATACCAAACTCTG-3¢, corresponding to the N-terminal sequence (Met1– Cys9) of Arabidopsis CBCP, and the antisense primer 5¢-CAGCACCAAATGCCCAGCAAGAACC-3¢, corres-...

Ngày tải lên: 16/03/2014, 04:20

... 4). Activities of purified arginase and H. pylori arginase- containing extracts with divalent cations Mammalian arginases require manganese for optimal catalytic activity, although some of these arginases ... that RocF is neces- sary and sufficient for arginase activity, and that the enzyme has a number of interesting and unique features among the arginase superfamily, inclu...

Ngày tải lên: 16/03/2014, 16:20

... 2000 times in Tris-buffered saline. After several washes, the membrane was incubated with alkaline phosphatase conju- gated goat anti-rabbit Igs at a dilution of 1 : 30 000. The bands were visualized ... acid [30] using bovine serum albumin as a standard. Results Isolation and purification Polydocanol at a concentration of 0.9% released 70% of ATP/ADPase active protein fr...

Ngày tải lên: 17/03/2014, 10:20

... Iijima et al.(Eur. J. Biochem. 270) Ó FEBS 2003 Purification and characterization of three isoforms of chrysophsin, a novel antimicrobial peptide in the gills of the red sea bream, Chrysophrys major Noriaki ... Immunohistochemical localization of chrysophsins in the gills of the red sea bream. Adjacent sections in the gills were...

Ngày tải lên: 23/03/2014, 20:22

... lyase; acharan sulfate lyase; acharan sulfate; heparin. Heparin, heparan sulfate and acharan sulfate glycosamino- glycans (GAGs) are comprised of alternating 1–4-linked glucosamine and uronic acid ... by the milligrams of protein in the cuvette. Protein concentra- tion was measured by a Bradford assay using bovine serum albumin as a standard [18]. Characterization of sal...

Ngày tải lên: 23/03/2014, 21:20