Báo cáo khoa học: Properties of purified gut trypsin from Helicoverpa zea, adapted to proteinase inhibitors pdf
Báo cáo khoa học: Properties of purified gut trypsin from Helicoverpa zea, adapted to proteinase inhibitors pdf
... World-Wide-Web-
accessible tool. Electrophoresis 18, 403–408.
Ó FEBS 2003 Helicoverpa trypsins (Eur. J. Biochem. 270)19
Properties of purified gut trypsin from
Helicoverpa zea
,
adapted to proteinase inhibitors
Mariateresa ... ranging from 1 n
M
to 30 n
M
.Thus,
HzTrypsin-S seems to allow the insect to overcome different
defensive proteinase inhibitors in pla...
Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx
... JH
potentiators of EcR–USP, then receptors from an
insect species such as the Colorado potato beetle,
Leptinotarsa decemlineata, are expected to evoke a pro-
file of response that varies considerably from ... activity
of muristerone A in the receptors from both species, but juvenile hormone
III was unable to potentiate the inductive activity of the diacylhydrazine
methoxyfeno...
Tài liệu Báo cáo khoa học: Properties of the recombinant glucose⁄galactose dehydrogenase from the extreme thermoacidophile, Picrophilus torridus ppt
... and 215 (P. torridus glucose
dehydrogenase numbering), which are reported to
explain the dual cofactor specificity of the enzyme
from T. acidophilum.
Cloning and expression of the P. torridus glucose
dehydrogenase ... the
crude extract of P. torridus DSM 9790 cells. The glucose dehydrogenase
was partially purified from P. torridus cells and identified by MS to be
identical with t...
Báo cáo khoa học: Properties of two multifunctional plant fatty acid acetylenase/desaturase enzymes pptx
... (Merck).
Gels from polar lipid areas (application spots) were scraped
off and methylated in situ with 0.1
M
sodium methoxide .
Fatty acid methyl esters of total lipids from chloroform
extracts of yeast ... bond of 9(Z),12(E)-
octadecadienoic acid took place by selective removal o f
the pro-R hydrogen from C-12 and the pro-S hydrogen
from C-13.
It is well established from studi...
Tài liệu Báo cáo khoa học: Dimers of light-harvesting complex 2 from Rhodobacter sphaeroides characterized in reconstituted 2D crystals with atomic force microscopy docx
... organization of the photosyn-
thetic apparatus of Rhodobacter sphaeroides. EMBO J
18, 534–542.
12 Drews G & Imhoff JF (1991) Phototrophic purple bac-
teria. In Variations in Autotrophic Life ... the
formation of functional photosynthetic domains and membrane curvature.
To further investigate in detail the packing effects of like-protein photosyn-
thetic complexes, we report an a...
Tài liệu Báo cáo khoa học: Role of cleavage and shedding in human thyrotropin receptor function and trafficking pdf
...
Results
Generation of truncated mutants corresponding either
to b-subunits of the TSHR, or to a receptor deleted
of almost the entire extracellular domain
To understand the role of receptor cleavage ... arrestins, or due
to an easier clustering of the activated receptors in coated
pits [44].
It has to be noted that in the case of the M453T mutant
receptor, contrary to...
Tài liệu Báo cáo khoa học: Characterization of a chemosensory protein (ASP3c) from honeybee (Apis mellifera L.) as a brood pheromone carrier pdf
... was analyzed by MALDI-TOF mass spectrometry.
Two microlitres of purified ASP3c were mixed with 2 lLof
matrix solution (saturated solution of sinapinic acid in 30%
v/v acetonitrile, 0.2% v/v trifluoroacetic ... aliquots were added to 1 mL of
1 l
M
ASP3c solution using a 1-lL Hamilton syringe.
Dissociation constants (K
d
) were calculated from a plot of
fluorescence intensity vs. con...
Báo cáo khoa học: Characterization of membrane-bound prolyl endopeptidase from brain ppt
... by trypsin digestion of purified
membrane PE were analyzed by liquid chromatogra-
phy–MS ⁄ MS on Qstar (HPLC ⁄ Q ⁄ TOF) or MALDI-
TOF ⁄ TOF MS, which revealed that these fragments
correspond to ... The
amounts of protein loaded onto gels were as follows: for the PSD-
95 blot, 70 lg of TM, 96 lg of LM, 89 lg of MM, and 93 lgof
HM; for the calnexin blot, 70 lg of TM, 96 lg of...
Báo cáo khoa học: Characterization of myo-inositol hexakisphosphate deposits from larval Echinococcus granulosus pdf
... protoscolex of Echinococcus granulosus. J Parasitol
53, 312–325.
11 Richards KS (1984) Echinococcus granulosus equinus:
the histochemistry of the laminated layer of the hydatid
cyst. Folia Histochem Cytobiol ... Carbohydrates were estimated as the sum
of masses of galactose, N-acetylgalactosamine and N-acetylgluco-
samine, which together make up over 90% of the total LL sugar
([12...
Báo cáo khoa học: Promoters of type I interferon genes from Atlantic salmon contain two main regulatory regions docx
... into the pCR-XL-TOPO vector
(Invitrogen, Carlsbad, CA, USA).
Promoter constructs
Various constructs of the promoter region of SasaIFN-A1
and SasaIFN-A2 were PCR-cloned into the pGL3-basic
vector ... 100 lL
nucleofector solution T (Amaxa Biosystems). A total of
15 lg plasmid was added, and the mixture was transferred
to a cuvette and transfected with program T-20 in the
nucleofector de...